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pcDNA3.1(+)-Histac-H3K9/K14 (#RDB14340)

For the detection of acetylation at the 9th and 14th lysine residues of histone H3 by the BRD4 bromodomain.

Clone info. Expression vector of Histac-H3K9/K14, a fluorescent probe to visualize acetylation of histone H3 Lys9 (H3K9) and histone H3 Lys14 (H3K14) in living cells by means of fluorescence resonance energy transfer (FRET), under the control of CMV promotor. See Fig. 1A of Nakaoka, S. et. al., ACS Chem. Biol. 11 (3): 729-733, 2016.
Comment Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone pcDNA3.1 (Plasmid)
Size of vector backbone 5.4 kb
Gene/insert name Human BRD4 cDNA Mouse Histone 3/n cDNA
Depositor|Developer Sasaki, Kazuki |
 
Sequence (full) RDB14340hts01.seq checkAssembled from experimentally sequenced data.
Publication Nakaoka, S., ACS Chem. Biol. 11 (3): 729-733 (2016). PMID 25946208. [link to RRC of NBRP]
Test sheet RDB14340_A7I6p1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakaoka, S. et al., ACS Chem. Biol., 11 (3), 729-733, 2006)
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakaoka, S. et al., ACS Chem. Biol., 11 (3), 729-733, 2006)。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14340 pcDNA3.1(+)-Histac-H3K9/K14 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(+)-Histac-H3K9/K14 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14340).

Reference section:

Nakaoka, S., Sasaki, K., Ito, A., Nakao, Y., Yoshida, M., A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733 (2016). PMID 25946208. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakaoka, S., A Genetically Encoded FRET Probe to Detect Intranucleosomal Histone H3K9 or H3K14 Acetylation Using BRD4, a BET Family Member. ACS Chem. Biol. 11 (3): 729-733 (2016). PMID 25946208. [link to RRC of NBRP]
reference He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225. [link to Journal]
reference Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321. [link to Journal]
user_report Santoni, G., Chromatin plasticity predetermines neuronal eligibility for memory trace formation. Science 385 (6707): eadg9982 (2024). PMID 39052786. [link to RRC of NBRP]