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pBabe-hSTING(V147L)

Retroviral expression vector of human STING, V147L mutant.

Catalog number RDB14353
Resource name pBabe-hSTING(V147L)
Clone info. Mammalian retroviral vector for expressing human STING (mutation, V147L). The disease (SAVI) patients have a point mutation of STING (V147L, N154S or V155M). These STING variants exhibit a gain-of-function phenotype and are able to stimulate the production of an IFN beta reporter construct.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
Other clones in our bank

External Database
human TMEM173

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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          RDB14353 pBabe-hSTING(V147L) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14353_A6J7p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14353_A6J7a.seq check
          >D03866A3_A6J7_1_pBABE5'_A07_01_ABI08.ab1
              1 AGGGTCGGGA TCAGTGTGGT GGTACGTAGG AATTCGCCGC CACCATGCCC CACTCCAGCC
             61 TGCATCCATC CATCCCGTGT CCCAGGGGTC ACGGGGCCCA GAAGGCAGCC TTGGTTCTGC
            121 TGAGTGCCTG CCTGGTGACC CTTTGGGGGC TAGGAGAGCC ACCAGAGCAC ACTCTCCGGT
            181 ACCTGGTGCT CCACCTAGCC TCCCTGCAGC TGGGACTGCT GTTAAACGGG GTCTGCAGCC
            241 TGGCTGAGGA GCTGCGCCAC ATCCACTCCA GGTACCGGGG CAGCTACTGG AGGACTGTGC
            301 GGGCCTGCCT GGGCTGCCCC CTCCGCCGTG GGGCCCTGTT GCTGCTGTCC ATCTATTTCT
            361 ACTACTCCCT CCCAAATGCG GTCGGCCCGC CCTTCACTTG GATGCTTGCC CTCCTGGGCC
            421 TCTCGCAGGC ACTGAACATC CTCCTGGGCC TCAAGGGCCT GGCCCCAGCT GAGATCTCTG
            481 CACTGTGTGA AAAAGGGAAT TTCAACGTGG CCCATGGGCT GGCATGGTCA TATTACATCG
            541 GATATCTGCG GCTGATCCTG CCAGAGCTCC AGGCCCGGAT TCGAACTTAC AATCAGCATT
            601 ACAACAACCT GCTACGGGGT GCAGTGAGCC AGCGGCTGTA TATTCTCCTC CCATTGGACT
            661 GTGGGGTGCC TGATAACCTG AGTATGGCTG ACCCCAACAT TCGCTTCCTG GATAAACTGC
            721 CCCAGCAGAC CGGTGACCAT GCTGGCATCA AGGATCGGGT TTACAGCAAC AGCATCTATG
            781 AGCTTCTGGA GAACGGGCAG CGGGCGGGCA CCTGTGTCCT GGAGTACGCC ACCCCCTTGC
            841 AGACTTTGTT TGCCATGTCA CAATACAGTC AAGCTGGCTT TAGCCGGGAG GATAGGCTTG
            901 AGCAGGCCAA ACTCTTCTGC CGGACACTTG AGGACATCCT GGCAGATGCC CCTGAGTCTC
            961 AGAACAACTG CCCGCCTCAT TGCCTACCAG GAAACCTGCA GATGACAGCA GCTTCTCGCT
           1021 GGTCCCAGGA GTTCTCCGGC ACTTGCGCAG AGGAAAAGGA GAGTACTGTG GGCCAGCTTT
          1081 GAGACTCAGC GTGCCAGCTA CTCACGATGT TTCCAAGAGA GAGCCTGAGC T
          //
          Primer: PuroR-rev
          Sequence file: RDB14353_A6J7b.seq check
          >D03866A3_A6J7_1_PuroR-rev_C06_03_ABI08.ab1
              1 GAATGTGCTT TTGCAAAGCC TAGGCCTCCA AAAAGCCTCC TCACTACTTC TGGAATAGCT
             61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG
            121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA
            181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG
            241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG
            301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG
            361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG
            421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC
            481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT
            541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG
            601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG
            661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG
            721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC
            781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG
            841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC
            901 AGCCGCTGGC TCACTGCACC CCGTAGCAGG TTGTTGTAAT GCTGATTGTA AGTTCGAATC
            961 CCGGGCCTGG AGCTCTGGCA GGATCAGCCC GCAGATATCC GATGTAATAT GACCATGCCA
           1021 GCCCATGGGC CACGTTTGAA ATTCCCCTTT TTCACACAGT GCAGAGATCT CAGCTGGGGC
           1081 CAGGCCCCTT GGAGACCAGA GATGTTCAGT GCTTGCAGAG CAGGAAGCAG CATCAGTGAA
          1141 GGCCGGCCGA ACCGCCAAAG
          //
          Primer: Amp_R
          Sequence file: RDB14353_A6J7c.seq check
          >D03866A3_A6J7_1_Amp_R_C03_09_ABI24.ab1
              1 TTCCCCTTTT TATAAATTTA ATTGAAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT
             61 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA
            121 AAGTGCCACC TGCAGCCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC
            181 CCCGGCTCAG GGCCAAGAAC AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG
            241 GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG GTCCCCAGAT GCGGTCCAGC
            301 CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT GCCCCAAGGA CCTGAAATGA
            361 CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT
            421 GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC GGGGCGCCAG TCCTCCGATT
            481 GACTGAGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC TTGCAGTTGC ATCCGACTTG
            541 TGGTCTCGCT GTTCCTTGGG AGGGTCTCCT CTGAGTGATT GACTACCCGT CAGCGGGGGT
            601 CTTTCATTTG GGGGCTCGTC CGGGATCGGG AGACCCCTGC CCAGGGACCA CCGACCCACC
            661 ACCGGGAGGC AAGCTGGCCA GCAACTTATC TGTGTCTGTC CGATTGTCTA GTGTCTATGA
            721 CTGATTTTAT GCGCCTGCGT CGGTACTAGT TAGCTAACTA GCTCTGTATC TGGCGGACCC
            781 GTGGTGGAAC TGACGAGTTC TGAACACCCG GCCGCAACCC TGGGAGACGT CCCAGGGACT
            841 TTGGGGGCCG TTTTTGTGGC CCGACCTGAG GAAGGGAAGT CGATGTGGAA TCCGACCCCG
            901 TCAGGATATG TGGGTTCTGG GTAGGGAGAC GAGAAACCTA AAACAGGTTC CCGCCTTCCG
            961 TCTGAAATTT TTTGCCTTTC GGGTTTGGGA ACCCGAAGCC GCGCGTTCTT GTCTGCCTGC
           1021 AACCGCTTGC AGCAATCGGT TCTGTGTTTG TCTCTTGTCC TGACTGTGTT CTGTTATTTG
           1081 GTCTGGAAAA AATTAAGGGC CAGAACTTGT TAACCACCTC CCTTCAGCTT TGAACCTTAA
          1141 AGTCACTGGA AAAGAATGGT CCGAGGAGCG GAATTGCGGT CT
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14353_A6J7d.seq check
          >D03866A3_A6J7_1_SV40pro_F_V2_C07_03_ABI08.ab1
              1 GTCCAACTTT TTTTGGGAGC CTAGGCTTTT GCAAAAGCTT ACCATGACCG AGTACAAGCC
             61 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
            121 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
            181 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
            241 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
            301 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
            361 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
            421 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
            481 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
            541 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
            601 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
            661 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATAAAATAAA AGATTTTATT
            721 TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA
            781 AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA AGTTCAGATC
            841 AAGGTCAGGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
            901 TTCCTGCCCC GGCTCAGGCC AAGAACAGAT GGAAACAGCT GAATATGGGC CAAACAGGAT
            961 ATCTGTGGTA AGCAGTTCCT GCCCCGGGCT CAGGCCAAGA AACAGGATGG GTCCCCAGAT
           1021 GCGTCCCAGC CCTCAGCCAG GTTTCTAGAA GAACCATCAG GATGTTTCAG GGTGCCCCAA
          1081 CGACTGAATG AACCTGTGCT ATTTGGACCT AACCAATTCC AGATTCGCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl