RDB14359 - pPmatLuc1

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pPmatLuc1 (#RDB14359)

Luciferase of Pyrocoelia matsumurai firefly with higher luminous intensity.

Clone info.	Luciferase of Pyrocoelia matsumurai firefly with higher luminous intensity. pPmatLuc1 is not expression vector. When the luciferase is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone	pUC19 (Plasmid)
Size of vector backbone	2.7 kb
Selectable markers	Amp^r
Growth conditions	LB+Amp, 37oC
Gene/insert name	Pyrocoelia matsumurai Luciferase cDNA
Depositor\|Developer	OLYMPUS CORPORATION, \| Ogoh, Katsunori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2012. Firefly luciferase. Japan patent publication 2012-235756). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Akiyoshi R, Ogoh K, Suzuki H. 2012. Firefly luciferase. Japan patent publication 2012-235756). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use() based on the results from the use of the BIOLOGICAL RESOURCE. ()Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ　特開2012-235756)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動()を行う場合は、オリンパス株式会社と別途協議を行う。()商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (ホタル由来ルシフェラーゼ　特開2012-235756)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14359	pPmatLuc1	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPmatLuc1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14359).

Reference section:

Ogoh, K., Akiyoshi, R., Suzuki, H., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]

Akiyoshi, R., Ogoh, K., Suzuki, H., Firefly luciferase. Japan Patent Abstracts 2012-235756. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14359_A6Jcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 Sequence file: RDB14359_A6Jca.seq
>D03707A1_A6Jc_2_Reverse2_B04_04_ABI24.ab1 1 CATCATCATT ACGCCAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC ACCATGGAAG 61 ATGACCACAA GAACATCGTG CACGGCCCTG CCCCATTCTA CCCCCTGGAA GAGGGAACAG 121 CCGGCGAGCA GCTGCACCGG GCCATGAAGA GATATGCCCA GGTGCCCGGC ACAATCGCCT 181 TCACCGATGC CCACGTGGAA GTGAACATCA CCTACAGCGA GTACTTCGAG ATGGCCTGCC 241 GGCTGGCCGA GACAATGAAG CGCTATGGCC TGGGCCTGCA GCACCACATT GCCGTGTGCA 301 GCGAGAACAG CCTGCAGTTC TTCATGCCCG TGTGTGGCGC CCTGTTCATC GGAGTGGGAG 361 TGGCCCCCAC CAACGACATC TACAACGAGA GAGAGCTGTA CAACAGCCTG AGCATCAGCC 421 AGCCCACCAT CGTGTTCTGC AGCAAGCGGG CCCTGCAGAA AATCCTGGGC GTGCAGAAAA 481 AGCTGCCCGT GATCGAGAAG ATCGTGATCC TGGACAGCCG CGAGGACTAC ATGGGCAAGC 541 AGAGCATGTA CAGCTTCATC GAGAGCCATC TGCCCGCTGG CTTCAACGAG TACGACTACG 601 TGCCCGACAC CTTCGACAGA GAGACAGCCA CCGCCCTGAT CATGAACAGC AGCGGCTCTA 661 CCGGCCTGCC CAAGGGCGTG GAACTGACCC ACAAGAATGT GTGCGTGCGG TTCAGCCACT 721 GCCGGGACCC TGTGTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTG ACCGTGATCC 781 CCTTCCACCA CGGCTTCGGC ATGTTCACCA CCCTGGGCTA CCTGACCTGC GGCTTCCGGA 841 TCGTGCTGAT GTACAGATTC GAGGAAGAAC TGTTCCTGCG GAGCCTGCAG GACTACAAGA 901 TCCAGAGCGC CCTGCTGGTG CCTACCCTGT TCAGCTTCTT CGCCAAGAGC ACCCTGGTGG 961 ATAAGTACGA CCTGAGCAAC CCTGCACGAG ATCGCCTCTG GGCGGAGCCC CCCCCTGGCT 1021 AAGGAAGGTG GGAGAGCCCG TTGGTCAGCC GGATTCAAGC TGCCGTGGCA TCAGAACAGG 1081 ACTTACGTCT TGACCGAAGC ACAACCCGTC G //
Primer: pUC-M13_-100 Sequence file: RDB14359_A6Jcb.seq
>D03707A1_A6Jc_2_pUC-M13_-100_A04_01_ABI24.ab1 1 GGCCTGTCAG GCCGATTAGT TGGGTACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC 61 GACGGCCAGT GAATTCTCAC AGCTTGCTTT TCTTGCCCAT TGTCAGCATC TCGCGGATCT 121 TCCGGCTGTC GATCTTGCCT GTCAGGCCCT TTGGCACCTC GTCCACGAAC TTCACGCCGC 181 CTCTCAGCCT CTTGCTGGCG GTCACTTGTC CGGCCACGTA GTCCATCACT TCCTGCTCGG 241 TCATGGTCTT GCCCTCTTCC AGCACCACCA CAGCGGCAGG CAGTTCGCCA GCATCAGCAT 301 CAGGGATGCC GGCCACCCCG GCATCGAAGA TGAAGGGATG CTGCAGCAGG ATAGATTCCA 361 GCTCGGCGGG TGGCACCTGG TAGCCCTGGT ACTTGATCAG GGACTTCAGC CGGTCCACGA 421 TGAAGAAGTG GCCGTCCTTG TCGTAGTAGG CCAGGTCGCC AGAGTGCAGC CAGCCATCCT 481 TGTCGATCAG GGCATTGGTG GCCTCGGGGT TGTTCACGTA GCCCTTCATA ATCATGGGGC 541 CCTTCAGACA CAGCTCGCCC CTCTGGTTCA CGCCCAGTGT CTTGCTGGTG TCCAGGTCCA 601 CAATCTTGGC GCTGAAAAAT GGGGCCACCT TTCCACAGGC GCCAGGCTTA TCGTCGCCCC 661 TGGGGGTGAT GATCACGGCA GAGGTTGTCT CGGTCAGGCC GTAGCCCTGT CTGATGCCAG 721 GCAGCTTGAA CCGCTTGGCC ACGGCCTCTC CCACTTCTTT AGCCAGGGGG GCTCCGCCAG 781 AGGCGATCTC GTGCAGGTTG CTCAGGTCGT ACTTATCCAC CAGGGTGCTC TTGGCGAAGA 841 AGCTGAACAG GGTAGGCACC AGCAGGGCGC TCTGGATCTT GTAGTCCTGC AGGCTCCGCA 901 GGAACAGTTC TTCCTCGAAT CTGTACATCA GCACGATCCG GAAGCCGCAG TCAAGGTAGC 961 CCAGGTGGTG AACATGCCGA AGCCGTGGGT GGAATGGGGA TCACGGGTCC AGGAATAGCG 1021 GTGTCGGGGA TGATCTGGGA TTGCCGACAC ACGGTCCCGG CAGTGACCTG ATCGCACGCC 1081 ACAACATTCT TTGTTGGGTT CAAGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ogoh, K., Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of engrailed expression during Drosophila metamorphosis. Biochem. Biophys. Rep. 23: 100771 (2020). PMID 32490216. [link to RRC of NBRP]
original	Akiyoshi, R., Firefly luciferase. Japan Patent Abstracts 2012-235756. [link to RRC of NBRP]
reference	Nakashiba, T., Development of two mouse strains conditionally expressing bright luciferases with distinct emission spectra as new tools for in vivo imaging. Lab. Anim. (NY) 52 (10): 247-257 (2023). PMID 37679611. [link to RRC of NBRP]
review	Suzuki, H., Bioluminescence microscopy for the visualization of gene expression patterns and the study of embryonic development. Proc. Arthropod. Embryol. Soc. Jpn. 53: 1-7 (2021).
user_report	Yamamoto, K., In vivo imaging identified efficient antimicrobial treatment against Mycobacterium marinum infection in mouse footpads. Sci. Rep. 14 (1): 24343 (2024). PMID 39420066. [link to RRC of NBRP]