Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCoGFP-Th1wt

Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 1.

Catalog number RDB14364
Resource name pCoGFP-Th1wt
Clone info. Thermostable green fluorescent protein of sea cactus Cavernularia obesa. Mutant 1. pCoGFP-Th1wt is not expression vector. When the fluorescent protein is expressed in any cells, the cDNA must be transferred to appropriate expression vectors by your own.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Cavernularia obesa GFP cDNA
Depositor|Developer OLYMPUS CORPORATION, | Ogoh, Katsunori |
Other clones in our bank

External Database
Cavernularia obesa GFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR is requested (Murai M, Ogoh K, Kinebuchi T, Suzuki H. Thermostable fluorescent protein and its utility. Japan patent publication 2014-60947). RECIPIENT must contact the OLYMPUS CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose [Word]
            MTA, for use for for-profit purpose [Word]
            Please visit Information of Request for Distribution.[link] 
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって別紙に定める寄託者の指定する文献を引用する (熱安定性を有する蛍光タンパク質およびそれを利用する方法 特開2014-60947)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、オリンパス株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            提供同意書 (MTA, 営利目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB14364 pCoGFP-Th1wt DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCoGFP-Th1wt was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14364).

            Reference section:

            Murai, M., Ogoh, K., Kinebuchi, T., Suzuki, H., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14364_A6Jcp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100
            Sequence file: RDB14364_A6Jca.seq check
            >D03707A6_A6Jc_1_pCU_M13-100_C01_07_ABI24.ab1
                1 GTATGTCAAG GCGGATTAAG TTGGGTACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA
               61 CGACGGCCAG TGAATTCGAG CTCGGTACCC GGGGATCCAT GAGTATTCCA GAGAATTCGG
              121 GCTTAACAGA AGAGATGCCT GCTCAAATGA ATTTAGAAGG AGTGGTAAAT GGTCATGCCT
              181 TCTCTATGGA AGGAATTGGT GGAGGGAACA TACTAACTGG AATTCAAAAG CTGGATATCC
              241 GTGTCACCGA GGGAGATCCT CTTCCGTTTT CCTTTGACAT TCTCTCAGTT GCGTTTCAAT
              301 ATGGCAACAG AACTTACACA AGTTATCCTG CGAAGATCCC CGATTATTTC GTTCAATCAT
              361 TCCCAGAAGG GTTCACCTTT GAAAGGACTC TTAGTTTTGA AGATGGTGCA ATCGTAAAGG
              421 TCGAATCGGA TATTAGTATT GAAGATGGCA AATTTGTTGG CAAGATTAAA TATAATGGCG
              481 AAAGTTTTCC AGAGGATGGA CCGGTGATGA AAAAGGAAGT TATCAAACTA GAACCGTCCA
              541 GTGAGTCAAT GTATGTACGT GACGACACTT TGGTGGGAGA AGTTGTGTTG TCTTACAAGA
              601 CCCAGTCGAC TCATTACACT TGCCATATGA AAACAATCTA CAGGTCCAAA AAGCCAGTAG
              661 AAAACCTGCC AAAGTTCCAT TATGTTCATC ATCGCCTCGA AAAGAAAAAA GTCGAGGAAG
              721 GCAACTACTA CGAGCAACAT GAGACGGCCA TAGCTAAACC ATGAAAGCTT GGCGTAATCA
              781 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA
              841 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT
              901 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCCAGC TGCATTAATG
              961 AATCGGCCAA CGCGCGGGGG AGAGGCGGTT TGCGTATTGG GCCGCTCCTT CCGCCTTCCT
             1021 CGCTCACTGA CCTCGCCTGC CGCCTCCGGT CCGTTCCGGC CTGCCGCCGA GCCGATATCC
             1081 AAGCCTCCCA ACTCCAAGCC GTATACCGGT TTATTCCCAC ACAGAAGTTC CAGGGGGGGA
            1141 TAC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Murai, M., Thermostable fluorescent protein and its utility. Japan Patent Abstracts 2014-60947. [link to RRC of NBRP]

            2023.10.04

            GNP_filter3_RDBDEP_html_231001.pl