Resource data sheet
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pCIneoFHF-PP1A (#RDB14397)

Expression vector of FLAG- and His6-tagged human PP1A.

Clone info. Expression vector of N-terminal FLAG-His6-FLAG-fused human protein phosphatase 1 catalytic subunit alpha (PP1A).
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human PPP1CA cDNA
Depositor|Developer Hata, Yutaka |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nagashima, S. et al. Cancer Sci. 107 (6), 791-802, 2016) and an acknowledgment to the DEPOSITOR are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nagashima, S. et al. Cancer Sci. 107 (6), 791-802, 2016) を引用し、寄託者への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14397 pCIneoFHF-PP1A Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCIneoFHF-PP1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14397).

Reference section:

Nagashima, S., Maruyama, J., Kawano, S., Iwasa, H., Nakagawa, K., Ishigami-Yuasa, M., Kagechika, H., Nishina, H., Hata, Y., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852. [link to RRC of NBRP]