Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgG2b

Retroviral expression vector of mouse Immunoglobulin G2b (IgG2b).

Catalog number RDB14505
Resource name pMXs-IRES-GFP_IgG2b
Clone info. Retroviral expression vector of mouse Immunoglobulin G2b (IgG2b), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighg2b cDNA
Depositor|Developer Kitamura, Daisuke |
Other clones in our bank

External Database
mouse Ighg2b

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) を引用し、寄託者への謝辞の表明を必要とする。
          付加的提供条件:
          pMXsベクターについて:利用者は、研究成果の公表にあたって pMXsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄先生への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14505 pMXs-IRES-GFP_IgG2b DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14505_A6Lep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14505_A6Lea.seq check
          >D04073A5_A6Le_1_pBABE5'_C01_07_ABI24.ab1
              1 GTCTTATATG GCCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG
             61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT
            121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC
            181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA
            241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG
            301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC
            361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCCCAGTGT GGTGGTACGG GAATTCACAC
            421 AGGACCTCAC CATGGGATGG AGCTGTATCA TGCTCTTCTT GGCAGCAACA GCTACAGGTG
            481 TCCACTCCCA GGTCCAACTG CAGCAGCCTG GGGCTGAGCT TGTGAAGCCT GGGGCTTCAG
            541 TGAAGCTGTC CTGCAAGGCT TCTGGCTACA CCTTCACCAG CTACTTGATG CACTGGGTGA
            601 AGCAGAGGCC TGGACGAGGC CTTGAGTGGA TTGGAAGGAT TGATCCTAAT AGTGGTGGTA
            661 CTAAGTACAA TGAGAAGTTC AAGAGCAAGG CCACACTGAC TGTAGACAAA CCCTCCAGCA
            721 CAGCCTACAT GCAGCTCAGC AGCCTGACAT CTGAGGACTC TGCGGTCTAT TATTGTGCAA
            781 GATATGATTA CTACGGTAGT AGCTACTTTG ACTACTGGGG CCAAGGCACC ACTCTCACAG
            841 TCTCGACTGC CAAAACAACA CCCCCATCAG TCTATCCACT GGCCCCTGGG TGTGGAGATA
            901 CAACTGGTTC CTCTGTGACT CTGGGGATGC CTGGTCAAGG GCTACTTCCC TGAGTCAGTG
            961 ACTGTGACTT GGAACTCTGG ATCCCTGTCC AGCAGTGTGC ACACCTTCCC AGCTCTCCTG
           1021 CAGTCTGGAC TCTACACTAT GAGCAGCTCA GTGACTGTCC CCTCCAGCAC CTGGCCAAGT
           1081 CAGACCGTCA CCTGCAGCGT TGCTCACCCA GGCAGGCAGC AACCACGGTG GTAACAAAAA
          1141 ACTTGAAGCC CAGGCCGGGG CCCCCACAAT CA
          //
          Primer: IRES-R
          Sequence file: RDB14505_A6Leb.seq check
          >D04073A5_A6Le_1_IRES-R_E02_14_ABI24.ab1
              1 GCCATCAGCG GCTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
             61 ATTTACGTAG CGGCCGCCTA GGCTCCCTGC CCAATCATGT TTCTGTAGTC AGGGGAGATC
            121 TTCTGCTTCA GCTCCACCAC TGAGGAGAAG ATCCACTTTA CCTTGAAGAG TGTGACAGAG
            181 GCGCTGTAGC ACACGCTGAG CAGGAAGAGG CTGATGAAGA TGGTGATGGT CGTCCAGAGC
            241 CCGTCCAGCT CCCCATCCTT GGCCTCAGCA CAGATATCAT CCAGGTCTAG CCCCGGAGAC
            301 CGGGAGATGG TCTTCTTCAG GTAGTAATTT TTCAGACCCT CGTGTCTCAC GTTGCATGAG
            361 AAGGAATCTG TTTTCTCCCA CTTGCTTGTT TTTATATCGA GCTTGCTGTA TATGAAGTAA
            421 GAACCGTCAG AGTCCAGGAC TGGTGCGGTG TCCTTGTAGT TCTCCTCTGT ATGCCCATTG
            481 CTGGTCCACT CCACACTGAT GTCTCCAGGG TTGAAGCCCA CGACCAGGCA AGTGAGACTG
            541 ACATCTTTCC TGGACAACTG CTCTGCTGGT GGCGGCAAGA TGTATACTTG TGGAGCTCTG
            601 ACTAGCCCTT TAATTTTTGA GATGGTTCTC TCGATGGGTG ATGGGAGGTC TTTGTTGTTG
            661 ACCTTGCATT TGAACTCCTT GCCACTCATC CAGTCCTGGT GCTGGATGGG GAGGGCACTG
            721 ACCACCCGGA TAGTACTGTT GTAATCCTCT CTATGGGTTT GTGTCTGAGC TGTGTGTACT
            781 TCCACGTTGT TCACAAACCA GCTGATCCGG ACGTCTGGGT CATCCTCGCT CACATCCACC
            841 ACCACACACG TGACCTTGGG TGTCAGGGAG ATCATGAGTA CATCCTTGAT ATTTGGAGGA
            901 AAGATGAAGA CGGATGGTCC ACCCTCGAGG TTAGGAGCTG GGCATTTGTG ACACTCCCTT
            961 GCATGGAGGA CAGGGGTTGA TTGTTGAAAT GGGCCCGCTG GGCTCAAGTT TTTTGTCCAC
           1021 CGTGGTGCTG CTGGCTGGGT GAGCACGCTG CAGTGACGTC TGACTTGCCA GTGCTTGAAG
           1081 GGACAGTCAC TGAGCTGCTC ATAGGTAAGT TCAGACTGCA GGAAGCCTGG CAGTGTTGCA
          1141 CACTTGCCTT GGAACAGCA
          //
          Primer: IRES-F
          Sequence file: RDB14505_A6Lec.seq check
          >D04073A5_A6Le_1_IRES-F_C08_08_ABI24.ab1
              1 GGCAGTGCTT TAAATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
             61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG
            121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
            181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
            241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
            301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
            361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
            421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
            481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
            541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA
            601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC
            661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC
            721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
            781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCGGCCGC
            841 CAGCACAGTG GTCGACGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA AAGGGGGGAA
            901 TGAAAGACCC CACCTGTAAG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TTGCAAGGCA
            961 TGGAAAAATA CATAACTGAG AAATAGAGAA GTTCAGATCA AAGGTCAGAA CAGATGGAAA
           1021 CAGCTGAATA TGGCCCAAAC AGGATATCTG TGGTAGCAGT TCCTGCCCCC GGCTTCAGGT
          1081 CAGAACAGGA TTGGAACAGC CTGAATATTG GCCCAAACCA AGG
          //
          Primer: Amp_R
          Sequence file: RDB14505_A6Led.seq check
          >D04073A5_A6Le_1_Amp_R_D08_11_ABI24.ab1
              1 CTTCCTTTTT TCATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
             61 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
            121 GCCACCTGCA TAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA
            181 TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAAA AGTTCAGATC AAGGTCAGGA
            241 ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC
            301 GGCTCAGGGC CAAGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
            361 AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCAGCCCT
            421 CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC
            481 TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT
            541 CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATTGAC
            601 TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG
            661 TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT
            721 TCATTTGGGG GCTCGTCCGG GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC
            781 GGGAGGTAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG
            841 ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG
            901 GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG GAGACGTCCC AGGGACTTCG
            961 GGGGGCCGTT TTTGTGGCCC GACCTGAAGT CCAAAAATCC CGATCGTTTT GGGACTCTTT
           1021 GGTGCACCCC CCTTTAGAAG AGGGATATGT GATCCTGTAA GAGACGAAGA CTTAAACAGA
          1081 TCCCGGCCTC CGTCCTGAAT TTTTGCCATT CCGAATTTTG G
          //
          Primer: SV40pA3'element Reverse
          Sequence file: RDB14505_A6Lee.seq check
          >D04073A5_A6Le_1_SV40pA3element Reverse_E08_14_ABI24.ab1
              1 GTAGGCGGGG GGGCGGACCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
             61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
            121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
            181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
            241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
            301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
            361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
            421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
            481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
            541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
            601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
            661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
            721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
            781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
            841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
            901 CAGGTGGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAA TCTTTTTATT
            961 TTTATCGTCG ACCCACTGTG CTGGGCGGCC CGGCCGCTTT TACTTGTACA GCTCGTTCCA
           1021 TGCCGAGAGG TGATTCCCGG CGGCGGTCAC GACTCCAGCA GACCAATGTG ATCGCGCATT
          1081 CTCGGTTGGG GATCTTTTGG CTTCAAGGGA CCGGGAACCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl