RDB14513 - pMXs-IRES-hNGFR

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding G-CaMP6: (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Ohkura, M., PLoS One 7 (12): e51286, 2012) and an acknowledgment are requested. (2) The DEVELOPER may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEVELOPER. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution. Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内)

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献（Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016）を引用し、寄託者への謝辞の表明を必要とする。付加的提供条件： G-CaMP6について：(1) 利用者は、研究成果の公表にあたって文献（Ohkura, M., PLoS One 7 (12): e51286, 2012) を引用し、謝辞の表明を必要とする。(2) 開発者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に開発者の求めに対して回答することとする。(3) 当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。 pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

References and tips

QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14513	pMXs-IRES-hNGFR_G-CaMP6	DNA solution

original	Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

Featured content	Calcium Ion Sensor Clones (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

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Primer: pBABE5' Sequence file: RDB14513_A6Lea.seq
>D04073B4_A6Le_1_pBABE5'_G01_19_ABI24.ab1 1 GTCCTTATAT GGCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG 61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT 121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC 181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA 241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG 301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC 361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTCGCCAC CATGCGGGGT TCTCATCATC 421 ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG 481 ACGATGACGA TAAGGATCTC GCCACCATGG TCGACTCATC ACGTCGTAAG TGGAATAAGA 541 CAGGTCACGC AGTCAGAGCT ATAGGTCGGC TGAGCTCACT CGAGAACGTC TATATCAAGG 601 CCGACAAGCA GAAGAACGGC ATCAAGGCGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG 661 GCGGCGTGCA GCTCGCCTAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC 721 TGCTGCCCGA CAACCACTAC CTGAGCGTGC AGTCCAAACT TTCGAAAGAC CCCAACGAGA 781 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG 841 ACGAGCTGTA CAAGGGCGGT ACCGGAGGGA GCATGGTGAG CAAGGGCGAG GAGCTGTTCA 901 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG 961 TGTCCGGCGA GGGTGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA 1021 CCACCCGGCA AGCTGCCCGT GCCCTGGCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC 1081 AGTGCCTTCA GCCGCTACCC CGACACATGA GGCCAGCACG ACCTCTCAGT CGGTCATGCC 1141 CGAAGCTACA GTCCACGAAG CGCAAGCAAT CGCTG //
Primer: IRES-R Sequence file: RDB14513_A6Leb.seq
>D04073B4_A6Le_1_IRES-R_E03_15_ABI24.ab1 1 GCATCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA 61 ATTTACGTAG CGGCCGCTCA CTTCGCTGTC ATCATTTGTA CAAACTCTTC GTAGTTTACC 121 TGACCATCCC CATCGATGTC TGCTTCCCTG ATCATTTCAT CAACCTCTTC ATCTGTTAAC 181 TTCTCTCCAA GGTTTGTCAT CACGTGGCGA AGCTCTGCTG CACTGATGTA GCCATTGCCA 241 TCCTTATCAA ACACACGGAA CGCTTCTCTA ATTTCTTCTT CACTGTCTGT GTATTTCATT 301 TTTCTTGCCA TCATTGTCAG GAACTCAGGG AAGTCGATTG TGCCATCACC GTCGGCATCT 361 ACTTCATTGA TCATGTCCTG CAGCTCTGCT TCTGTGGGGT TCTGCCCCAG AGACCGCAGC 421 ACCGTCCCCA GCTCCTTGGT TGTTATTGTC CCATCCCCGT CCTTGTCAAA TAGGGAGAAA 481 GCCTCTTTAA ATTCTGCGAT CTGCTCTTCA GTCAGTTGGT CACGCGTGTT GTACTCCAGC 541 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCAC GATGCGGTTC 601 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTAGCC GTCGTCCTTG 661 AAGAAGATGG TGCGCTCCTG GATGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC 721 TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG 781 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG 841 CCGTAGGTGG CATCGCCCTC ACCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG 901 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC 961 ATGCTCCCTC CGGTACCGCC CTTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGGCGG 1021 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTGGGGTCTT TCGAAGTTTG 1081 ACTGCACGCT CAGTAGTTGA TTGTCAGCAG CCAGCACGGG GCCGTTCGCC GAATGGGGTG 1141 TTCCTGCTGT ATGTAGACAG CCTGCACGGA CCCG //
Primer: IRES-F Sequence file: RDB14513_A6Lec.seq
>D04073B4_A6Le_1_IRES-F_H10_22_ABI24.ab1 1 TGCATCATTT ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG 61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGA CGGGCCGCGC 121 CTGCTGCTGT TGCTGCTTCT GGGGGTGTCC CTTGGAGGTG CCAAGGAGGC ATGCCCCACA 181 GGCCTGTACA CACACAGCGG TGAGTGCTGC AAAGCCTGCA ACCTGGGCGA GGGTGTGGCC 241 CAGCCTTGTG GAGCCAACCA GACCGTGTGT GAGCCCTGCC TGGACAGCGT GACGTTCTCC 301 GACGTGGTGA GCGCGACCGA GCCGTGCAAG CCGTGCACCG AGTGCGTGGG GCTCCAGAGC 361 ATGTCGGCGC CGTGCGTGGA GGCCGACGAC GCCGTGTGCC GCTGCGCCTA CGGCTACTAC 421 CAGGATGAGA CGACTGGGCG CTGCGAGGCG TGCCGCGTGT GCGAGGCGGG CTCGGGCCTC 481 GTGTTCTCCT GCCAGGACAA GCAGAACACC GTGTGCGAGG AGTGCCCCGA CGGCACGTAT 541 TCCGACGAGG CCAACCACGT GGACCCGTGC CTGCCCTGCA CCGTGTGCGA GGACACCGAG 601 CGCCAGCTCC GCGAGTGCAC ACGCTGGGCC GACGCCGAGT GCGAGGAGAT CCCTGGCCGT 661 TGGATTACAC GGTCCACACC CCCAGAGGGC TCGGACAGCA CAGCCCCCAG CACCCAGGAG 721 CCTGAGGCAC CTCCAGAACA AGACCTCATA GCCAGCACGG TGGCAGGTGT GGTGACCACA 781 GTGATGGGCA GCTCCCAGCC CGTGGTGACC CGAGGCACCA CCGACAACCT CATCCCTGTC 841 TATTGCTCCA TCCTGGCTGC TGTGGTTGTG GGCCTTGTGG CCTACATAGC CTTCAAGAGG 901 TGGAACAGCT GCAAGCAGAA CAAGCAAGGA GCCAACAGCT GAGTCGACGA TAAATAAAAG 961 ATTTTATTTA GTCTCCAGAA AAAGGGGGGA AATGAAAGAC CCCACCCTGT AGTTTGGGCA 1021 AGGCTAGCTT AGTACGGCCA ATTTTTGGCA AGCAATGAAA AATTACATTA ACCTTGAGAT 1081 TAGAGAGTCC AGACTCAAGT CAAGAACAGA ATTGGAACCA //
Primer: Amp_R Sequence file: RDB14513_A6Led.seq
>D04073B4_A6Le_1_Amp_R_F11_17_ABI24.ab1 1 ATTAGTTTTT CATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT 181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA 241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG 301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA 361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC 421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT 481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC 541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT 601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT 661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT 721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG 781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA 841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG 901 TGGAACTGAC GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG 961 GGGCCGTTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG ATCGTTTTGG ACTCTTTTGG 1021 GTGCACCCCC CCTTTAGAGG AGGGATAATG TGGTTTCTGT AGAGACGAAG AACTTAAACA 1081 GTCAGCCTTC GGTCTGATTT TTTGGCCTTT CCGGATTGGG GGAATC //
Primer: SV40pA3'element Reverse Sequence file: RDB14513_A6Lee.seq
>D04073B4_A6Le_1_SV40pA3element Reverse_G11_20_ABI24.ab1 1 GCGCGGCCAG GGGGGCGGAG CCTATGGAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 241 GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG TAAATACTTA AGAAAAAAAA 301 CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC GCTGACGGGT AGTCAATCAC 361 TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC GGATGCAACT GCAAGAGGGT 421 TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG AGGACTGGCG CCCCGAGTGA 481 GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC GCGCGAACAG AAGCGAGAAG 541 CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT CAGGTCCTTG GGGCACCCTG 601 GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA CCGCATCTGG GGACCATCTG 661 TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA TATCCTGTTT GGCCCATATT 721 CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA ACTGCTTACC ACAGATATCC 781 TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT TGATCTGAAC TTCTCTATTC 841 TCAGTTATGT ATTTTTTCCA TGCCTTGCAA AATGGCGTTA CTTAAGCTAG CTTGCCAAAC 901 CTACAGGTGG GGTCTTTCAT TCCCCCCCTT TTTCTGGAGA CTAAATAAAA TCTTTTTATT 961 TTATCGTCGA CTCAGCTGTT GGGCTCCTTG CTTTGTTTCT GCTTGCAGCT GTTCCAACCT 1021 CTGAAGCTAT GTAGGGCCAC AAGCTCCACA ACCACCAGCA GCCAAGGATG AGCCAATAGA 1081 CAGGGAATGA ACGTTGGTCC CGGATGAGGT TTGAACT //

pMXs-IRES-hNGFR_G-CaMP6

Distribution information

References and tips

QC test results

References