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pGL4_ifng-2500-luc (#RDB14515)

Reporter construct of mouse interferon gamma (Ifng) promoter (-2500 to +113).

Clone info. Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-2500 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-2500-luc, the PCR product (2.6 kb) was amplified with forward primer (5' CTA CTC GAG AAA TGG GAC ACA GAT CTC 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector. Genomic DNA fragment 118,438,542 to 118,441,154 nt of NC_000076.6 is cloned. Known differences (at least): C deletion at 118,438,965 nt; A insertion between 118,440,965 and 118,440,966 nt.
Vector backbone pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Ifng genomic DNA
Depositor|Developer Kataoka, Takao |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14515 pGL4_ifng-2500-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4_ifng-2500-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14515).

Reference section:

Fukuoka, N., Harada, M., Nishida, A., Ito, Y., Shiota, H., Kataoka, T., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14515_A6Lfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB14515_A6Lfa.seq check
>D03972A1_A6Lf_1_pGL4-4174F_A07_01_ABI08.ab1
    1 GCGGGTCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGAAA
   61 TGGGACACAG ATCTCAACAG AGAATTCTCC ATAGTGAAAA GTCACATGGC TGAGAAACAC
  121 TTCAATGTTT AACATCCTTA GCCATTAGGG AAATGCAATT CAAAACTTCT CTGAGATCCC
  181 ATCTTACATC TGTCAGAATG TCTAAGATCA ATAAGACACG TGACAGCTCA CACTGGTGTG
  241 GAGCAAGGGG AACACTCTCC TACTGAGGTT GGTAGTGCAA ATATCACTTT TAAAAAATTA
  301 ATAGAGAACA TAGAACGGTC CCCGTCATCA GGTGTTAGCT AACGAGACCA CACCATTTTC
  361 TAGCATTAAT GGTAAATTAC ATTTAATAAA AAAGCTGATT TCCTTCAAAC TAGAAGGAAT
  421 AGCATTGGAA CTGTTAAAAA ACTTGAGGGC AATAACTCAA ACTAGATTCC CCCCCCCCCC
  481 AGAAAGGGTT TCACTGTGTA GCTCTGGTTA TCCTGGAACT CACTCTGTAG ACCAGGATAA
  541 CCTCAAATTC ACAGAGATTG CTGGCTTCTG TCACCTGAGT GCTGGGATTA AAGGTATGAG
  601 TTACCACGAC CCTGCTAGAT TTTTTTTTTA AGACAGATTC TCATGATTCT TAAACTGGCC
  661 TTCAAGTCTC CAGCAGCAGA AGGTGACCTC GCACTCATGA TCATCTTACC TCTGTTTCCA
  721 TCTGTATTTC ATGCCTATTT TAAGTCAGGG TTTTTATACA AGCACTCTAC CAACCAAGAG
  781 ATGTTCCTGA CTGTCAAAAC AGAGATTCTT AGGTAAACAT TCAAATGAAT ATTTGTGTGT
  841 GTGTGTGTGT TGTTTGTGTA TAAATCTTTA TGCTTATGTA TTTGAGGGAA ATAAATTGTG
  901 TCACAGAAAG CAATGAAACA CTTTTCTGGT TTTAAAGGAA ATTAGTGGGG CACTGGGATG
  961 GGGGGTAGGG GGGCATGCAT AGTTACCCTC GTATATGAGA CTGGGGGAAA TTTGAGAACC
 1021 CATGAAGGCA TAGAACAAAG TGCTAGCCCT GCCCAGAGGG ATAAAGTCTG ATTTGCAGGC
1081 CTGGAGGCTG GGGGAGACTC AACAGAGGAT TGGATTTGCC AT
//
Primer: Luc_R ()
Region: insert 3'
Sequence file: RDB14515_A6Lfb.seq check
>D03972A1_A6Lf_1_Luc_R_B07_02_ABI08.ab1
    1 AGTTAAAGGG TTCCGGGGCA CAGGGCGTAG CGCTTCATGG CTTTGTGCAG CTGCTCGCCG
   61 GCGGTCCCGT CTTCGAGTGG GTAGAATGGC GCTGGGCCCT TCTTAATGTT TTTGGCATCT
  121 TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT GGCCGCCGAG GCCAGATCTT
  181 GATATCTGTC TCAGAGCTAG GCCGCAGGAG GAGAAGCCCA GAACTTCTGT CTCAAGTCAG
  241 AGGGTCCAAA GGATCAGCTG ATGTGTCTTC TCTAGGTCAG CCGATGGCAG CTATAGCCTG
  301 CACCTCTCTG GCTTCCAGTT TTATACCTGA TCGAAGGCTC CTCGGGATTA CGTATTTTCA
  361 CAAGTTTTTT TTTGGTTTTT TTTTTTTTTT TAAGATGGTG ACAGATAGGT GGGGGGAGCT
  421 AAGTTACTTT GCATTACAGC TATGGTTTTG TGGCATGTTA GAGTTTCCTT TCGACTCCTT
  481 GGGCTCTCTG ACGATGAGAC AGCCCCGCTG TGCCCACCTG TGCCATTCTT GTGGGATTCT
  541 CTGAAAGCAC TTTTACTTCA CACCATTTGG GGTGGGGGCT GGAAATTGCT TTTACGTCTC
  601 CCCCTTTTTG CCCTTGTAAT GTGAATTTCT CATCCACAGA GCACAGCACA GGGAGCCTTT
  661 GTTTGTGGTT GGGGACGGCT GAATAAAAGG AAACTCTTGG GCTTCTCAAA CCATGAGGAA
  721 ACTCTTGGGC TTCTCAAACC ATGCAATGGG GGAAGCCTTT TCAAACTTTT CTTGCAAATG
  781 ACCAGAAAAG CAAGGAAAGG GTATGGTTAA AAAGCCAATG TGGTGAGGAA GCCCTTCAAT
  841 GGAGGTGATT AGTAAAATAC CTACTACAAA TCACTCAGGG GTCAACGTGC CCAGAAAGAA
  901 GAAAAGGCAG ACCACATAGG TAAAATAAAA TCATCTAGTT TCCTCCATTA ATAAAAGGGA
  961 AAAATGCAAA TGAATAAAAG TAAAAATGCA GAATACTTTC ATTAGATATT GTATTTCACT
 1021 TCAACACACC CAAGTATCAA TGTGTAATCC AGATTTATGG ATGAATAAGA GTTTTCAATT
1081 CCTCTGGCAT TTAAGTATTT GAAGGTCCTA GATTTGC
//
Primer: SV40pro_R2 ()
Region: Luciferase 3'
Sequence file: RDB14515_A6Lfc.seq check
>D03972A1_A6Lf_1_SV40pro_R2_C09_09_ABI24.ab1
    1 TGTCAGGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG
   61 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGAAGGTA GCTAACCAAG TTCCTCTTTC
  121 AGAGGTTATT TCAGGCCATG GTGCTGCGCA GATCAGCGGA AGAGCGCCCA ATACGCAAAC
  181 GGATCCTTAT CGATTTTACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC
  241 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG
  301 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
  361 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGCT
  421 CGAAGCGGCC GGCCGCCCCG ACTCTAGACT GCAGTTAGAC GTTGATCCTG GCGCTGGCGC
  481 AAGCAGCAGG GTGTCTATCC ATGCCGCTCT CCTGGGCGCA GCTCATGGGC AGGGTGCCGG
  541 CGGCCTGCTC CTCCACCTCG GGAGGGAAGC CGTGGCTGTT AAGGTGGATC ACAAAGTGGC
  601 TTAAGCTACT GAACCAGTTC TTGCAAGCAG AATTCACGGC GATCTTGCCG CCCTTCTTGG
  661 CCTTAATGAG AATCTCGCGG ATCTTGCGGG CGTCCAACTT GCCGGTCAGT CCTTTAGGCA
  721 CCTCGTCCAC GAACACAACA CCACCGCGCA GCTTCTTGGC GGTTGTAACC TGGCTGGCCA
  781 CATAGTCCAC GATCTCCTTC TCGGTCATGG TTTTACCGTG TTCCAGCACG ACGACTGCGG
  841 CGGGCAGCTC GCCGGCATCG TCGTCGGGCA GGCCGGCGAC CCCGGCGTCG AAGATGTTGG
  901 GGTGTTGCAG CAGGATGCTC TCCAGTTCGG CTGGGGCTAC CTGGTAGCCC TTGTATTTGA
  961 TCAGGCTCTT CAGCCGGTCC ACGATGAAGA AGTGCTCGTC CTCGTCCCAG TAGCGATGTC
 1021 GCCGCTGTGC AGCCAGCCGT CCTTTGTCGA TGAGAGCGTT TGTAGCCCTC GGGTGTACGT
 1081 AGCCGCTCAT GAATCATTGG GGCCACGACG CACAGCTCGC GCGCTGATCA CAACTAGTTC
1141 CTACCGGAAT GTTACGTCAG AGAGT
//
Primer: SV40pro_F ()
Region: SV40 pro/ori, PuroR, poly(A) signal
Sequence file: RDB14515_A6Lfd.seq check
>D03972A1_A6Lf_1_SV40pro_F_C07_03_ABI08.ab1
    1 CCCCGTCTTT GCATCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCGATTCT TCTGACACTA GCGCCACCAT GACCGAGTAC AAGCCTACCG TGCGCCTGGC
  121 CACTCGCGAT GATGTGCCCC GCGCCGTCCG CACTCTGGCC GCCGCTTTCG CCGACTACCC
  181 CGCTACCCGG CACACCGTGG ACCCCGACCG GCACATCGAG CGTGTGACAG AGTTGCAGGA
  241 GCTGTTCCTG ACCCGCGTCG GGCTGGACAT CGGCAAGGTG TGGGTAGCCG ACGACGGCGC
  301 GGCCGTGGCC GTGTGGACTA CCCCCGAGAG CGTTGAGGCC GGCGCCGTGT TCGCCGAGAT
  361 CGGCCCCCGA ATGGCCGAGC TGAGCGGCAG CCGCCTGGCC GCCCAGCAGC AAATGGAGGG
  421 CCTGCTTGCC CCCCATCGTC CCAAGGAGCC TGCCTGGTTT CTGGCCACTG TAGGAGTGAG
  481 CCCCGACCAC CAGGGCAAGG GCTTGGGCAG CGCCGTCGTG TTGCCCGGCG TAGAGGCCGC
  541 CGAACGCGCC GGTGTGCCCG CCTTTCTCGA AACAAGCGCA CCAAGAAACC TTCCATTCTA
  601 CGAGCGCCTG GGCTTCACCG TGACCGCCGA TGTCGAGGTG CCCGAGGGAC CTAGGACCTG
  661 GTGTATGACA CGAAAACCTG GCGCCTAATG ATCTAGAACC GGTCATGGCC GCAATAAAAT
  721 ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GTTCGAACTA GATGCTGTCG
  781 ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
  841 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC
  901 AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGGCGA
  961 GCGGTATCAG CTCACTCAAA GGCGGGTAAT ACGGGTTATC CACAGAAATC ATGGGATACG
 1021 CAGCAAGGAC CATGTGGAGC CAAAATCCCA GCAAAATGTC AGGAATCGTA AAAACGGCCC
1081 GCCGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]