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pGL4_ifng-2000-luc (#RDB14516)

Reporter construct of mouse interferon gamma (Ifng) promoter (-2000 to +113).

Clone info. Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-2000 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-2000-luc, the PCR product (2.1 kb) was amplified with forward primer (5' CTA CTC GAG ATT GCT GGC TTC TGT CAC CTG 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector. Genomic DNA fragment 118,439,039 to 118,441,154 nt of NC_000076.6 is cloned. Known differences (at least): A deletion at 118,441,150 nt; A insertion between 118,440,965 and 118,440,966 nt.
Vector backbone pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Ifng genomic DNA
Depositor|Developer Kataoka, Takao |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14516 pGL4_ifng-2000-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4_ifng-2000-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14516).

Reference section:

Fukuoka, N., Harada, M., Nishida, A., Ito, Y., Shiota, H., Kataoka, T., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14516_A6Lfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB14516_A6Lfa.seq check
>D03972A2_A6Lf_1_pGL4-4174F_D07_04_ABI08.ab1
    1 ATAGGGGTCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGA
   61 TTGCTGGCTT CTGTCACCTG AGTGCTGGGA TTAAAGGTAT GAGTTACCAC GACCCTGCTA
  121 GATTTTTTTT TTAAGACAGA TTCTCATGAT TCTTAAACTG GCCTTCAAGT CTCCAGCAGC
  181 AGAAGGTGAC CTCGCACTCA TGATCATCTT ACCTCTGTTT CCATCTGTAT TTCATGCCTA
  241 TTTTAAGTCA GGGTTTTTAT ACAAGCACTC TACCAACCAA GAGATGTTCC TGACTGTCAA
  301 AACAGAGATT CTTAGGTAAA CATTCAAATG AATATTTGTG TGTGTGTGTG TGTTGTTTGT
  361 GTATAAATCT TTATGCTTAT GTATTTGAGG GAAATAAATT GTGTCACAGA AGCAATGAAA
  421 CACTTTCTGG TTTTAAGGAA ATTAGTGGGC ACTGGGATGG GGGTAGGGGG CATGCATAGT
  481 TACCTCGTAT ATGAGACTGG GGAAATTGAG AACCCATGAA GCATAGAAAC AAAGTGCTAG
  541 CCCTGCCCAG AGGATAAAGT CTGATTTGAA GCTGGAGGCT TGGGGGAGAA CCAAAACAGA
  601 GGATGGTTTG CATCTGGGTC AAGATAACTG GGTACCACTC TGAGATGCTC AGATGAGGGA
  661 AAGGGGGCAC ATGGCCAAAG GAACTGCACG AACAAGCTGC ATAGCGTTGT TAAACATCTG
  721 ACATTGCCAA ATAACCATCA AGTTTTAATT GAGCCACTAG GAATGCCGGG AAGAATTCTC
  781 TCAACTTTCC ATGGAGCATT TGGTTGAGCC TTTGGCTGAA GTCCGGAAGA AAATGCTAGT
  841 GTTAAGCTCA CTGTATAAAT GTGGAAACTG AGTCACAGAT CATAAAGATG CTGACTCAAG
  901 ACCCCGAGGC TAGAATATGA ATGGTTTCAA GTCTGCACCC ATAGCCACTG TGTAATGTTT
  961 AATGAATACA GGAAGGCAGG GATGATACAC GTGGGCTGCA CTGGAGAGCA GAGGTCTCCT
 1021 GCCCCAATCT TCACGAATCG GAATTTCCAT AGATAAGGTA CTAAGGATGA GCAGAACCTT
1081 TTATGGTGAC TTGATGCGAA GCAACTAT
//
Primer: Luc_R ()
Region: insert 3'
Sequence file: RDB14516_A6Lfb.seq check
>D03972A2_A6Lf_1_Luc_R_E07_05_ABI08.ab1
    1 ACGAATGGTG CCGGGCACAG CGCGTAGCGC TTCATGGCTT TGTGCAGCTG CTCGCCGGCG
   61 GTCCCGTCTT CGAGTGGGTA GAATGGCGCT GGGCCCTTCT TAATGTTTTT GGCATCTTCC
  121 ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC CGCCGAGGCC AGATCTTGAT
  181 ATCTGTCCAG AGCTAGGCCG CAGGAGGAGA AGCCCAGAAC TTCTGTCTCA AGTCAGAGGG
  241 TCCAAAGGAT CAGCTGATGT GTCTTCTCTA GGTCAGCCGA TGGCAGCTAT AGCCTGCACC
  301 TCTCTGGCTT CCAGTTTTAT ACCTGATCGA AGGCTCCTCG GGATTACGTA TTTTCACAAG
  361 TTTTTTTTTG GTTTTTTTTT TTTTTTTAAG ATGGTGACAG ATAGGTGGGG GGAGCTAAGT
  421 TACTTTGCAT TACAGCTATG GTTTTGTGGC ATGTTAGAGT TTCCTTTCGA CTCCTTGGGC
  481 TCTCTGACGA TGAGACAGCC CCGCTGTGCC CACCTGTGCC ATTCTTGTGG GATTCTCTGA
  541 AAGCACTTTT ACTTCACACC ATTTGGGGTG GGGGCTGGAA ATTGCTTTTA CGTCTCCCCC
  601 TTTTTGCCCT TGTAATGTGA ATTTCTCATC CACAGAGCAC AGCACAGGGA GCCTTTGTTT
  661 GTGGTTGGGG ACGGCTGAAT AAAAGGAAAC TCTTGGGCTT CTCAAACCAT GAGGAAACTC
  721 TTGGGCTTCT CAAACCATGC AATGGGGGAA GCCTTTTCAA ACTTTTCTTG CAAATGACCA
  781 GAAAAGCAAG GAAAGGGTAT GGTTAAAAAG CCAATGTGGT GAGGAAGCCC TTCAATGGAG
  841 GTGATTAGTA AAATACCTAC TACAAATCAC TCAGGGTCAA CGTGCCCAGA AAGAAGAAAA
  901 GGCAGACCAC ATAGGTAAAA TAAAATCATC TAGTTCCTCC ATTAATAAAA GGGAAAAAAA
  961 TGCAAAATGA ATAAAAGTAA AAATGCCAAG ATACTTTCAT TTAGATATTG TATTTCACTC
 1021 ACACACCCGA GTATCATGTG TATCAGGAAT TTATGATGGA TTAGAGTTCC ATTTCGTCTG
1081 CACTAGATTT AAGCCTAGTG CCTATTCCTA
//
Primer: SV40pro_R2 ()
Region: Luciferase 3'
Sequence file: RDB14516_A6Lfc.seq check
>D03972A2_A6Lf_1_SV40pro_R2_D09_12_ABI24.ab1
    1 AAAAATCCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG
   61 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGAAGGTA GCTAACCAAG TTCCTCTTTC
  121 AGAGGTTATT TCAGGCCATG GTGCTGCGCA GATCAGCGGA AGAGCGCCCA ATACGCAAAC
  181 GGATCCTTAT CGATTTTACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC
  241 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG
  301 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
  361 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGCT
  421 CGAAGCGGCC GGCCGCCCCG ACTCTAGACT GCAGTTAGAC GTTGATCCTG GCGCTGGCGC
  481 AAGCAGCAGG GTGTCTATCC ATGCCGCTCT CCTGGGCGCA GCTCATGGGC AGGGTGCCGG
  541 CGGCCTGCTC CTCCACCTCG GGAGGGAAGC CGTGGCTGTT AAGGTGGATC ACAAAGTGGC
  601 TTAAGCTACT GAACCAGTTC TTGCAAGCAG AATTCACGGC GATCTTGCCG CCCTTCTTGG
  661 CCTTAATGAG AATCTCGCGG ATCTTGCGGG CGTCCAACTT GCCGGTCAGT CCTTTAGGCA
  721 CCTCGTCCAC GAACACAACA CCACCGCGCA GCTTCTTGGC GGTTGTAACC TGGCTGGCCA
  781 CATAGTCCAC GATCTCCTTC TCGGTCATGG TTTTACCGTG TTCCAGCACG ACGACTGCGG
  841 CGGGCAGCTC GCCGGCATCG TCGTCGGGCA GGCCGGCGAC CCCGGCGTCG AAGATGTTGG
  901 GGTGTTGCAG CAGATGCTCT CCAGTTCGGC TGGGGCTACC TGGTAGCCCT TGTATTTGAT
  961 CAAGCTCTTC AGCCGGTCCA CGATGAAAGA AGTGCTCGTC CTCGTCCCAG TAGGCGATGT
 1021 CGCCGCTGTG CAGCCAGCCC GTCCCTTGTC GATGAGAGCG TTTGTAGCCC TCGGGGATGT
 1081 TAACGTAGCC GCCTCATGAA TCATGGGGCA CGAACGCCAC AGCTCGGACG CGCTGGTTCA
1141 ACCACCCAAG GTTGTCT
//
Primer: SV40pro_F ()
Region: SV40 pro/ori, PuroR, poly(A) signal
Sequence file: RDB14516_A6Lfd.seq check
>D03972A2_A6Lf_1_SV40pro_F_F07_06_ABI08.ab1
    1 CATCCTCTTT AACAATCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCGATT CTTCTGACAC TAGCGCCACC ATGACCGAGT ACAAGCCTAC CGTGCGCCTG
  121 GCCACTCGCG ATGATGTGCC CCGCGCCGTC CGCACTCTGG CCGCCGCTTT CGCCGACTAC
  181 CCCGCTACCC GGCACACCGT GGACCCCGAC CGGCACATCG AGCGTGTGAC AGAGTTGCAG
  241 GAGCTGTTCC TGACCCGCGT CGGGCTGGAC ATCGGCAAGG TGTGGGTAGC CGACGACGGC
  301 GCGGCCGTGG CCGTGTGGAC TACCCCCGAG AGCGTTGAGG CCGGCGCCGT GTTCGCCGAG
  361 ATCGGCCCCC GAATGGCCGA GCTGAGCGGC AGCCGCCTGG CCGCCCAGCA GCAAATGGAG
  421 GGCCTGCTTG CCCCCCATCG TCCCAAGGAG CCTGCCTGGT TTCTGGCCAC TGTAGGAGTG
  481 AGCCCCGACC ACCAGGGCAA GGGCTTGGGC AGCGCCGTCG TGTTGCCCGG CGTAGAGGCC
  541 GCCGAACGCG CCGGTGTGCC CGCCTTTCTC GAAACAAGCG CACCAAGAAA CCTTCCATTC
  601 TACGAGCGCC TGGGCTTCAC CGTGACCGCC GATGTCGAGG TGCCCGAGGG ACCTAGGACC
  661 TGGTGTATGA CACGAAAACC TGGCGCCTAA TGATCTAGAA CCGGTCATGG CCGCAATAAA
  721 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGTTCGAAC TAGATGCTGT
  781 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG
  841 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG
  901 GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
  961 AGCGGTATCA GCTCACTCAA GGCGGTAATA CGGTTATCCA CAGAAATCAG GGGAATACGC
 1021 AGAAGACAAT GTGAGCAAAT GTCAGCCAAA ATGCCAGGAA TCGTTAAAAG CGCGTATGGC
1081 TGGGCGATTT TTCCAAATAG GT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]