RDB14518 - pGL4_ifng-1000-luc

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pGL4_ifng-1000-luc

Reporter construct of mouse interferon gamma (Ifng) promoter (-1000 to +113).

Catalog number	RDB14518
Resource name	pGL4_ifng-1000-luc
Clone info.	Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-1000 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment	The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-1000-luc, the PCR product (1.1 kb) was amplified with forward primer (5' CTA CTC GAG CCA AAG ACA GAG AAG GAA AC 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector.
Vector backbone	pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name	mouse Ifng genomic DNA
Depositor\|Developer	Kataoka, Takao \|

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mouse Ifng

External Database
mouse Ifng

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14518	pGL4_ifng-1000-luc	DNA solution

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References and tips

Electronic file

Original reference

original	Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

Further references such as user reports and related articles (go to bottom)

Featured content	T cell receptor signaling pathway (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14518_A6Lfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert 5' Sequence file: RDB14518_A6Lfa.seq
>D03972A4_A6Lf_1_pGL4-4174F_B08_02_ABI08.ab1 1 AAGAGGTTCC GGAACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA 61 GCCAAAGACA GAGAAGGAAA CTTTTAGTGT TTGAACTTGA TGGGGGAAAC AATTTTCAGT 121 TATTAATCCT TATTTGGGAC AAGTGTGTGT CTAGTGCTGG ACTAGTCATT GGGATAGCCT 181 TAGCCACATG TGGGTATGAG AGTTAGAAAC ATGGCTAATC CTGAAGTGAT TTCTGCTGTG 241 TATATAGGAT AGCCACTAGG CTTTAAATAT TTAGTGGCAG AGGAATTGAA ACTCTTATTC 301 ATCATAAATC TTGATACACA TTGATACCTT GGGTGTGTTG AGTGAAATAC AATATCTAAT 361 GAAGTATTCT TGCATTTTTA CTTTTATTTC ATTTTGCATT TTTTCCCTTT TATTAATGGA 421 GGAACTAGAT GATTTTATTT TACCTATGTG GTCTGCCTTT TCTTCTTTCT GGGCACGTTG 481 ACCCTGAGTG ATTTGTAGTA GGTATTTTAC TAATCACCTC CATTGAAGGG CTTCCTCACC 541 ACATTGGCTT TTTAACCATA CCCTTTCCTT GCTTTTCTGG TCATTTGCAA GAAAAGTTTG 601 AAAAGGCTTC CCCCATTGCA TGGTTTGAGA AGCCCAAGAG TTTCCTCATG GTTTGAGAAG 661 CCCAAGAGTT TCCTTTTATT CAGCCGTCCC CAACCACAAA CAAAGGCTCC CTGTGCTGTG 721 CTCTGTGGAT GAGAAATTCA CATTACAAGG GCAAAAAGGG GGAGACGTAA AAGCAATTTC 781 CAGCCCCCAC CCCAAATGGT GTGAAGTAAA AAGTGCTTTC AGAGAATCCC ACAAGAATGG 841 CACAGGTGGG CACAGCGGGG GCTGTCTCAT CGTCAGAGAG CCCAAGGAGT CGAAAGGGAA 901 ACTCTAACAT GCCACAAAAC CATAGCTGTA ATGCAAGTAA CTTAGCTCCC CCCCACCTAT 961 CTGTCACCAT CTTAAAAAAA AAAAAAAACC AAAAAAAAAA CTTTGTGAAA ATACGTAATT 1021 CCCGAGGGAG CCTTCGAATC AGTATAAAAA CTTGAAAGCC AGGAGAGGTG CAAGGCCTTA 1081 TAGCTGCCAT TCGCCTGACT TAAGTAGGAC ACATCAGCCT GGAATCCTTT GGGGAACCCC 1141 CTCCG //
Primer: Luc_R Region: insert 3' Sequence file: RDB14518_A6Lfb.seq
>D03972A4_A6Lf_2_Luc_R_D11_04_ABI08.ab1 1 CGTCAAAGGG TGCCCGGGGA CCAGGGCGTA GCGCTTCATG GCTTTGTGCA GCTGCTCGCC 61 GGCGGTCCCG TCTTCGAGTG GGTAGAATGG CGCTGGGCCC TTCTTAATGT TTTTGGCATC 121 TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT TGGCCGCCGA GGCCAGATCT 181 TGATATCTGT CTCAGAGCTA GGCCGCAGGA GGAGAAGCCC AGAACTTCTG TCTCAAGTCA 241 GAGGGTCCAA AGGATCAGCT GATGTGTCTT CTCTAGGTCA GCCGATGGCA GCTATAGCCT 301 GCACCTCTCT GGCTTCCAGT TTTATACCTG ATCGAAGGCT CCTCGGGATT ACGTATTTTC 361 ACAAGTTTTT TTTTGGTTTT TTTTTTTTTT TAAGATGGTG ACAGATAGGT GGGGGGAGCT 421 AAGTTACTTT GCATTACAGC TATGGTTTTG TGGCATGTTA GAGTTTCCTT TCGACTCCTT 481 GGGCTCTCTG ACGATGAGAC AGCCCCGCTG TGCCCACCTG TGCCATTCTT GTGGGATTCT 541 CTGAAAGCAC TTTTACTTCA CACCATTTGG GGTGGGGGCT GGAAATTGCT TTTACGTCTC 601 CCCCTTTTTG CCCTTGTAAT GTGAATTTCT CATCCACAGA GCACAGCACA GGGAGCCTTT 661 GTTTGTGGTT GGGGACGGCT GAATAAAAGG AAACTCTTGG GCTTCTCAAA CCATGAGGAA 721 ACTCTTGGGC TTCTCAAACC ATGCAATGGG GGAAGCCTTT TCAAACTTTT CTTGCAAATG 781 ACCAGAAAAG CAAGGAAAGG GTATGGTTAA AAAGCCAATG TGGTGAGGAA GCCCTTCAAT 841 GGAGGTGATT AGTAAAATAC CTACTACAAA TCACTCAGGG TCAACGTGCC CAGAAAGAAG 901 AAAAGGCAGA CCACATAGGT AAAATAAAAT CATCTAGTTC CTCCATTAAT AAAAGGGAAA 961 AAATGCAAAA TGAAATAAAA GTAAAAATGC AAGAATACTT CATTAGATAT TTGTATTTCA 1021 CTCAACACAC CCAAGGTATC AATGTGTATC AAGGATTTAT GATGATAGAA GTTTCATTTC 1081 CTCTGCCACT AAATATTTAA GCCTAGTGGC TAATCCTAAT AATACACAGC AGAATCACTT 1141 TCAGGATTAG CCATGTCCTA ACTCTCTCAT ACCACATGTG GCTAAGCTAT CCCGAATTGA 1201 ACTATGATCT CGAAGCACCA TGA //
Primer: SV40pro_R2 Region: Luciferase 3' Sequence file: RDB14518_A6Lfc.seq
>D03972A4_A6Lf_1_SV40pro_R2_F09_18_ABI24.ab1 1 AAAATTCCTT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT 61 GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGAAGGT AGCTAACCAA GTTCCTCTTT 121 CAGAGGTTAT TTCAGGCCAT GGTGCTGCGC AGATCAGCGG AAGAGCGCCC AATACGCAAA 181 CGGATCCTTA TCGATTTTAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA 241 CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT 301 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT 361 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGC 421 TCGAAGCGGC CGGCCGCCCC GACTCTAGAC TGCAGTTAGA CGTTGATCCT GGCGCTGGCG 481 CAAGCAGCAG GGTGTCTATC CATGCCGCTC TCCTGGGCGC AGCTCATGGG CAGGGTGCCG 541 GCGGCCTGCT CCTCCACCTC GGGAGGGAAG CCGTGGCTGT TAAGGTGGAT CACAAAGTGG 601 CTTAAGCTAC TGAACCAGTT CTTGCAAGCA GAATTCACGG CGATCTTGCC GCCCTTCTTG 661 GCCTTAATGA GAATCTCGCG GATCTTGCGG GCGTCCAACT TGCCGGTCAG TCCTTTAGGC 721 ACCTCGTCCA CGAACACAAC ACCACCGCGC AGCTTCTTGG CGGTTGTAAC CTGGCTGGCC 781 ACATAGTCCA CGATCTCCTT CTCGGTCATG GTTTTACCGT GTTCCAGCAC GACGACTGCG 841 GCGGGCAGCT CGCCGGCATC GTCGTCGGGC AGGCCGGCGA CCCCGGCGTC GAAGATGTTG 901 GGGGTGTTGC AGCAGATGCT CTCCAGTTCG GCTGGGGCTA CCTGGTAGCC CTTGTATTTG 961 ATCAGGCTCT TCAGCCGGTC CACGATGAAG AAGTGCTCGT CCTCGTCCCA GTAGGCGATG 1021 TCGCCGCTGT GCAGCCAGCC GTCCCTGTCG ATGAGAGCGT TTGTAGCCCT TCGGGGGTGT 1081 ACGTAGCCGG CTCATGATCA TGGGGATACG AACGCCACAG CCTTCGTCGC GCTGGATTTC 1141 CACAACCCCA AAG //
Primer: SV40pro_F Region: SV40 pro/ori, PuroR, poly(A) signal Sequence file: RDB14518_A6Lfd.seq
>D03972A4_A6Lf_1_SV40pro_F_D08_04_ABI08.ab1 1 CTCTGGTCTT GGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 61 AAGCTCGATT CTTCTGACAC TAGCGCCACC ATGACCGAGT ACAAGCCTAC CGTGCGCCTG 121 GCCACTCGCG ATGATGTGCC CCGCGCCGTC CGCACTCTGG CCGCCGCTTT CGCCGACTAC 181 CCCGCTACCC GGCACACCGT GGACCCCGAC CGGCACATCG AGCGTGTGAC AGAGTTGCAG 241 GAGCTGTTCC TGACCCGCGT CGGGCTGGAC ATCGGCAAGG TGTGGGTAGC CGACGACGGC 301 GCGGCCGTGG CCGTGTGGAC TACCCCCGAG AGCGTTGAGG CCGGCGCCGT GTTCGCCGAG 361 ATCGGCCCCC GAATGGCCGA GCTGAGCGGC AGCCGCCTGG CCGCCCAGCA GCAAATGGAG 421 GGCCTGCTTG CCCCCCATCG TCCCAAGGAG CCTGCCTGGT TTCTGGCCAC TGTAGGAGTG 481 AGCCCCGACC ACCAGGGCAA GGGCTTGGGC AGCGCCGTCG TGTTGCCCGG CGTAGAGGCC 541 GCCGAACGCG CCGGTGTGCC CGCCTTTCTC GAAACAAGCG CACCAAGAAA CCTTCCATTC 601 TACGAGCGCC TGGGCTTCAC CGTGACCGCC GATGTCGAGG TGCCCGAGGG ACCTAGGACC 661 TGGTGTATGA CACGAAAACC TGGCGCCTAA TGATCTAGAA CCGGTCATGG CCGCAATAAA 721 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGTTCGAAC TAGATGCTGT 781 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG 841 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG 901 GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGGCTGCGGC 961 GAGCGGTATC AGCTCACTCA AAAGGCGGTA AATACGGGTT ATCCACAGGA ATCACGGGAT 1021 AACGCAGAAG AAACATGTGA GCCAAAAGTC AGCAAAAGGC CAGCATCGTA AAAAGGTCGC 1081 GTGCTGGACG ATTTTCCATT AAGGGCTTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl