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pGL4_ifng-1000-luc (#RDB14518)

Reporter construct of mouse interferon gamma (Ifng) promoter (-1000 to +113).

Clone info. Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-1000 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-1000-luc, the PCR product (1.1 kb) was amplified with forward primer (5' CTA CTC GAG CCA AAG ACA GAG AAG GAA AC 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector.
Vector backbone pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Ifng genomic DNA
Depositor|Developer Kataoka, Takao |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14518 pGL4_ifng-1000-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4_ifng-1000-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14518).

Reference section:

Fukuoka, N., Harada, M., Nishida, A., Ito, Y., Shiota, H., Kataoka, T., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14518_A6Lfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert 5'
Sequence file: RDB14518_A6Lfa.seq check
>D03972A4_A6Lf_1_pGL4-4174F_B08_02_ABI08.ab1
    1 AAGAGGTTCC GGAACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA
   61 GCCAAAGACA GAGAAGGAAA CTTTTAGTGT TTGAACTTGA TGGGGGAAAC AATTTTCAGT
  121 TATTAATCCT TATTTGGGAC AAGTGTGTGT CTAGTGCTGG ACTAGTCATT GGGATAGCCT
  181 TAGCCACATG TGGGTATGAG AGTTAGAAAC ATGGCTAATC CTGAAGTGAT TTCTGCTGTG
  241 TATATAGGAT AGCCACTAGG CTTTAAATAT TTAGTGGCAG AGGAATTGAA ACTCTTATTC
  301 ATCATAAATC TTGATACACA TTGATACCTT GGGTGTGTTG AGTGAAATAC AATATCTAAT
  361 GAAGTATTCT TGCATTTTTA CTTTTATTTC ATTTTGCATT TTTTCCCTTT TATTAATGGA
  421 GGAACTAGAT GATTTTATTT TACCTATGTG GTCTGCCTTT TCTTCTTTCT GGGCACGTTG
  481 ACCCTGAGTG ATTTGTAGTA GGTATTTTAC TAATCACCTC CATTGAAGGG CTTCCTCACC
  541 ACATTGGCTT TTTAACCATA CCCTTTCCTT GCTTTTCTGG TCATTTGCAA GAAAAGTTTG
  601 AAAAGGCTTC CCCCATTGCA TGGTTTGAGA AGCCCAAGAG TTTCCTCATG GTTTGAGAAG
  661 CCCAAGAGTT TCCTTTTATT CAGCCGTCCC CAACCACAAA CAAAGGCTCC CTGTGCTGTG
  721 CTCTGTGGAT GAGAAATTCA CATTACAAGG GCAAAAAGGG GGAGACGTAA AAGCAATTTC
  781 CAGCCCCCAC CCCAAATGGT GTGAAGTAAA AAGTGCTTTC AGAGAATCCC ACAAGAATGG
  841 CACAGGTGGG CACAGCGGGG GCTGTCTCAT CGTCAGAGAG CCCAAGGAGT CGAAAGGGAA
  901 ACTCTAACAT GCCACAAAAC CATAGCTGTA ATGCAAGTAA CTTAGCTCCC CCCCACCTAT
  961 CTGTCACCAT CTTAAAAAAA AAAAAAAACC AAAAAAAAAA CTTTGTGAAA ATACGTAATT
 1021 CCCGAGGGAG CCTTCGAATC AGTATAAAAA CTTGAAAGCC AGGAGAGGTG CAAGGCCTTA
 1081 TAGCTGCCAT TCGCCTGACT TAAGTAGGAC ACATCAGCCT GGAATCCTTT GGGGAACCCC
1141 CTCCG
//
Primer: Luc_R ()
Region: insert 3'
Sequence file: RDB14518_A6Lfb.seq check
>D03972A4_A6Lf_2_Luc_R_D11_04_ABI08.ab1
    1 CGTCAAAGGG TGCCCGGGGA CCAGGGCGTA GCGCTTCATG GCTTTGTGCA GCTGCTCGCC
   61 GGCGGTCCCG TCTTCGAGTG GGTAGAATGG CGCTGGGCCC TTCTTAATGT TTTTGGCATC
  121 TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT TGGCCGCCGA GGCCAGATCT
  181 TGATATCTGT CTCAGAGCTA GGCCGCAGGA GGAGAAGCCC AGAACTTCTG TCTCAAGTCA
  241 GAGGGTCCAA AGGATCAGCT GATGTGTCTT CTCTAGGTCA GCCGATGGCA GCTATAGCCT
  301 GCACCTCTCT GGCTTCCAGT TTTATACCTG ATCGAAGGCT CCTCGGGATT ACGTATTTTC
  361 ACAAGTTTTT TTTTGGTTTT TTTTTTTTTT TAAGATGGTG ACAGATAGGT GGGGGGAGCT
  421 AAGTTACTTT GCATTACAGC TATGGTTTTG TGGCATGTTA GAGTTTCCTT TCGACTCCTT
  481 GGGCTCTCTG ACGATGAGAC AGCCCCGCTG TGCCCACCTG TGCCATTCTT GTGGGATTCT
  541 CTGAAAGCAC TTTTACTTCA CACCATTTGG GGTGGGGGCT GGAAATTGCT TTTACGTCTC
  601 CCCCTTTTTG CCCTTGTAAT GTGAATTTCT CATCCACAGA GCACAGCACA GGGAGCCTTT
  661 GTTTGTGGTT GGGGACGGCT GAATAAAAGG AAACTCTTGG GCTTCTCAAA CCATGAGGAA
  721 ACTCTTGGGC TTCTCAAACC ATGCAATGGG GGAAGCCTTT TCAAACTTTT CTTGCAAATG
  781 ACCAGAAAAG CAAGGAAAGG GTATGGTTAA AAAGCCAATG TGGTGAGGAA GCCCTTCAAT
  841 GGAGGTGATT AGTAAAATAC CTACTACAAA TCACTCAGGG TCAACGTGCC CAGAAAGAAG
  901 AAAAGGCAGA CCACATAGGT AAAATAAAAT CATCTAGTTC CTCCATTAAT AAAAGGGAAA
  961 AAATGCAAAA TGAAATAAAA GTAAAAATGC AAGAATACTT CATTAGATAT TTGTATTTCA
 1021 CTCAACACAC CCAAGGTATC AATGTGTATC AAGGATTTAT GATGATAGAA GTTTCATTTC
 1081 CTCTGCCACT AAATATTTAA GCCTAGTGGC TAATCCTAAT AATACACAGC AGAATCACTT
 1141 TCAGGATTAG CCATGTCCTA ACTCTCTCAT ACCACATGTG GCTAAGCTAT CCCGAATTGA
1201 ACTATGATCT CGAAGCACCA TGA
//
Primer: SV40pro_R2 ()
Region: Luciferase 3'
Sequence file: RDB14518_A6Lfc.seq check
>D03972A4_A6Lf_1_SV40pro_R2_F09_18_ABI24.ab1
    1 AAAATTCCTT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT
   61 GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGAAGGT AGCTAACCAA GTTCCTCTTT
  121 CAGAGGTTAT TTCAGGCCAT GGTGCTGCGC AGATCAGCGG AAGAGCGCCC AATACGCAAA
  181 CGGATCCTTA TCGATTTTAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA
  241 CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG TTGTTGTTAA CTTGTTTATT
  301 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT
  361 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGC
  421 TCGAAGCGGC CGGCCGCCCC GACTCTAGAC TGCAGTTAGA CGTTGATCCT GGCGCTGGCG
  481 CAAGCAGCAG GGTGTCTATC CATGCCGCTC TCCTGGGCGC AGCTCATGGG CAGGGTGCCG
  541 GCGGCCTGCT CCTCCACCTC GGGAGGGAAG CCGTGGCTGT TAAGGTGGAT CACAAAGTGG
  601 CTTAAGCTAC TGAACCAGTT CTTGCAAGCA GAATTCACGG CGATCTTGCC GCCCTTCTTG
  661 GCCTTAATGA GAATCTCGCG GATCTTGCGG GCGTCCAACT TGCCGGTCAG TCCTTTAGGC
  721 ACCTCGTCCA CGAACACAAC ACCACCGCGC AGCTTCTTGG CGGTTGTAAC CTGGCTGGCC
  781 ACATAGTCCA CGATCTCCTT CTCGGTCATG GTTTTACCGT GTTCCAGCAC GACGACTGCG
  841 GCGGGCAGCT CGCCGGCATC GTCGTCGGGC AGGCCGGCGA CCCCGGCGTC GAAGATGTTG
  901 GGGGTGTTGC AGCAGATGCT CTCCAGTTCG GCTGGGGCTA CCTGGTAGCC CTTGTATTTG
  961 ATCAGGCTCT TCAGCCGGTC CACGATGAAG AAGTGCTCGT CCTCGTCCCA GTAGGCGATG
 1021 TCGCCGCTGT GCAGCCAGCC GTCCCTGTCG ATGAGAGCGT TTGTAGCCCT TCGGGGGTGT
 1081 ACGTAGCCGG CTCATGATCA TGGGGATACG AACGCCACAG CCTTCGTCGC GCTGGATTTC
1141 CACAACCCCA AAG
//
Primer: SV40pro_F ()
Region: SV40 pro/ori, PuroR, poly(A) signal
Sequence file: RDB14518_A6Lfd.seq check
>D03972A4_A6Lf_1_SV40pro_F_D08_04_ABI08.ab1
    1 CTCTGGTCTT GGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCGATT CTTCTGACAC TAGCGCCACC ATGACCGAGT ACAAGCCTAC CGTGCGCCTG
  121 GCCACTCGCG ATGATGTGCC CCGCGCCGTC CGCACTCTGG CCGCCGCTTT CGCCGACTAC
  181 CCCGCTACCC GGCACACCGT GGACCCCGAC CGGCACATCG AGCGTGTGAC AGAGTTGCAG
  241 GAGCTGTTCC TGACCCGCGT CGGGCTGGAC ATCGGCAAGG TGTGGGTAGC CGACGACGGC
  301 GCGGCCGTGG CCGTGTGGAC TACCCCCGAG AGCGTTGAGG CCGGCGCCGT GTTCGCCGAG
  361 ATCGGCCCCC GAATGGCCGA GCTGAGCGGC AGCCGCCTGG CCGCCCAGCA GCAAATGGAG
  421 GGCCTGCTTG CCCCCCATCG TCCCAAGGAG CCTGCCTGGT TTCTGGCCAC TGTAGGAGTG
  481 AGCCCCGACC ACCAGGGCAA GGGCTTGGGC AGCGCCGTCG TGTTGCCCGG CGTAGAGGCC
  541 GCCGAACGCG CCGGTGTGCC CGCCTTTCTC GAAACAAGCG CACCAAGAAA CCTTCCATTC
  601 TACGAGCGCC TGGGCTTCAC CGTGACCGCC GATGTCGAGG TGCCCGAGGG ACCTAGGACC
  661 TGGTGTATGA CACGAAAACC TGGCGCCTAA TGATCTAGAA CCGGTCATGG CCGCAATAAA
  721 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGTTCGAAC TAGATGCTGT
  781 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG
  841 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG
  901 GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGGCTGCGGC
  961 GAGCGGTATC AGCTCACTCA AAAGGCGGTA AATACGGGTT ATCCACAGGA ATCACGGGAT
 1021 AACGCAGAAG AAACATGTGA GCCAAAAGTC AGCAAAAGGC CAGCATCGTA AAAAGGTCGC
1081 GTGCTGGACG ATTTTCCATT AAGGGCTTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]