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pGL4_ifng-500-luc (#RDB14519)

Reporter construct of mouse interferon gamma (Ifng) promoter (-500 to +113).

Clone info. Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-500 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-500-luc, the PCR product (0.6 kb) was amplified with forward primer (5' CTA CTC GAG CCT TTC CTT GCT TTT CTG GTC 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector. Genomic DNA fragment 118,440,542 to 118,441,154 nt of NC_000076.6 is cloned. Known difference: A insertion between 118,440,965 and 118,440,966 nt.
Vector backbone pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Ifng genomic DNA
Depositor|Developer Kataoka, Takao |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14519 pGL4_ifng-500-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4_ifng-500-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14519).

Reference section:

Fukuoka, N., Harada, M., Nishida, A., Ito, Y., Shiota, H., Kataoka, T., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14519_A6Lfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F ()
Region: insert
Sequence file: RDB14519_A6Lfa.seq check
>D03972A5_A6Lf_1_pGL4-4174F_E08_05_ABI08.ab1
    1 GTATGGGGCC AGGACTTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
   61 CCTTTCCTTG CTTTTCTGGT CATTTGCAAG AAAAGTTTGA AAAGGCTTCC CCCATTGCAT
  121 GGTTTGAGAA GCCCAAGAGT TTCCTCATGG TTTGAGAAGC CCAAGAGTTT CCTTTTATTC
  181 AGCCGTCCCC AACCACAAAC AAAGGCTCCC TGTGCTGTGC TCTGTGGATG AGAAATTCAC
  241 ATTACAAGGG CAAAAAGGGG GAGACGTAAA AGCAATTTCC AGCCCCCACC CCAAATGGTG
  301 TGAAGTAAAA GTGCTTTCAG AGAATCCCAC AAGAATGGCA CAGGTGGGCA CAGCGGGGCT
  361 GTCTCATCGT CAGAGAGCCC AAGGAGTCGA AAGGAAACTC TAACATGCCA CAAAACCATA
  421 GCTGTAATGC AAAGTAACTT AGCTCCCCCC ACCTATCTGT CACCATCTTA AAAAAAAAAA
  481 AAAAACCAAA AAAAAACTTG TGAAAATACG TAATCCCGAG GAGCCTTCGA TCAGGTATAA
  541 AACTGGAAGC CAGAGAGGTG CAGGCTATAG CTGCCATCGG CTGACCTAGA GAAGACACAT
  601 CAGCTGATCC TTTGGACCCT CTGACTTGAG ACAGAAGTTC TGGGCTTCTC CTCCTGCGGC
  661 CTAGCTCTGA GACAGATATC AAGATCTGGC CCTCGGCGGC CAAAGCTTGG CAAATCCGGT
  721 ACTGTTGGTA AAGCCACCAT GGGAAGATGC CAAAAACATT AAGAAGGGGG CCCAACGCCA
  781 TTCTACCCAC TCGAAAAACG GGACCCGCCC GGCGAGCAGC TGCACAAAAG CCATTGAAAG
841 CGCCTAACGC CCCTGGGTGC CCCGGGCACC CATCC
//
Primer: SV40pro_R2 ()
Region: Luciferase 3'
Sequence file: RDB14519_A6Lfb.seq check
>D03972A5_A6Lf_1_SV40pro_R2_G09_21_ABI24.ab1
    1 AGTCGTGCCC TTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA
   61 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGAAG GTAGCTAACC AAGTTCCTCT
  121 TTCAGAGGTT ATTTCAGGCC ATGGTGCTGC GCAGATCAGC GGAAGAGCGC CCAATACGCA
  181 AACGGATCCT TATCGATTTT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
  241 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA
  301 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT
  361 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT
  421 GCTCGAAGCG GCCGGCCGCC CCGACTCTAG ACTGCAGTTA GACGTTGATC CTGGCGCTGG
  481 CGCAAGCAGC AGGGTGTCTA TCCATGCCGC TCTCCTGGGC GCAGCTCATG GGCAGGGTGC
  541 CGGCGGCCTG CTCCTCCACC TCGGGAGGGA AGCCGTGGCT GTTAAGGTGG ATCACAAAGT
  601 GGCTTAAGCT ACTGAACCAG TTCTTGCAAG CAGAATTCAC GGCGATCTTG CCGCCCTTCT
  661 TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA CTTGCCGGTC AGTCCTTTAG
  721 GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA ACCTGGCTGG
  781 CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC GTGTTCCAGC ACGACGACTG
  841 CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACCCCGGCG TCGAAAGATG
  901 TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGGCTGGG GCTACCTGGT AGCCCTTGTA
  961 TTTGATCAGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT CGTCCTCGAT CCCAGTAGGC
 1021 GATGTCGCCC GCTGTGCAGC CCAGCCGTCC CTTTGTCGAT GAGAGCGTTT TGTAGCCTCG
 1081 GGATTGTTAA CGGTAAGCCG CTCAATGGAT CCATTGGGTC CACGGAACGC AACAAGACTT
1141 CGCCCGCGCC TGGGTTTCCA CCACACCCCG AGT
//
Primer: SV40pro_F ()
Region: SV40 pro/ori, PuroR, poly(A) signal
Sequence file: RDB14519_A6Lfc.seq check
>D03972A5_A6Lf_1_SV40pro_F_B09_02_ABI08.ab1
    1 CCCCGGGCCT GGGGCATTCA AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCG ATTCTTCTGA CACTAGCGCC ACCATGACCG AGTACAAGCC TACCGTGCGC
  121 CTGGCCACTC GCGATGATGT GCCCCGCGCC GTCCGCACTC TGGCCGCCGC TTTCGCCGAC
  181 TACCCCGCTA CCCGGCACAC CGTGGACCCC GACCGGCACA TCGAGCGTGT GACAGAGTTG
  241 CAGGAGCTGT TCCTGACCCG CGTCGGGCTG GACATCGGCA AGGTGTGGGT AGCCGACGAC
  301 GGCGCGGCCG TGGCCGTGTG GACTACCCCC GAGAGCGTTG AGGCCGGCGC CGTGTTCGCC
  361 GAGATCGGCC CCCGAATGGC CGAGCTGAGC GGCAGCCGCC TGGCCGCCCA GCAGCAAATG
  421 GAGGGCCTGC TTGCCCCCCA TCGTCCCAAG GAGCCTGCCT GGTTTCTGGC CACTGTAGGA
  481 GTGAGCCCCG ACCACCAGGG CAAGGGCTTG GGCAGCGCCG TCGTGTTGCC CGGCGTAGAG
  541 GCCGCCGAAC GCGCCGGTGT GCCCGCCTTT CTCGAAACAA GCGCACCAAG AAACCTTCCA
  601 TTCTACGAGC GCCTGGGCTT CACCGTGACC GCCGATGTCG AGGTGCCCGA GGGACCTAGG
  661 ACCTGGTGTA TGACACGAAA ACCTGGCGCC TAATGATCTA GAACCGGTCA TGGCCGCAAT
  721 AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGTTCG AACTAGATGC
  781 TGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC
  841 ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG
  901 CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG
  961 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAAGGGGATA
 1021 ACGCAGGAAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
 1081 GCGTGCTGGC GTTTTTCCAT AGCTCGCCCC CCTGACGAGC ATCACAAAAA TCGAACGCTC
1141 AGTCAGAGGT TGGCGGAAGC CGGACCAGGA ACTAATAAAG AAATACCAGG GGCGGTTTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212. [link to RRC of NBRP]