RDB14519 - pGL4_ifng-500-luc

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pGL4_ifng-500-luc

Reporter construct of mouse interferon gamma (Ifng) promoter (-500 to +113).

Catalog number	RDB14519
Resource name	pGL4_ifng-500-luc
Clone info.	Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-500 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment	The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-500-luc, the PCR product (0.6 kb) was amplified with forward primer (5' CTA CTC GAG CCT TTC CTT GCT TTT CTG GTC 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector. Genomic DNA fragment 118,440,542 to 118,441,154 nt of NC_000076.6 is cloned. Known difference: A insertion between 118,440,965 and 118,440,966 nt.
Vector backbone	pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name	mouse Ifng genomic DNA
Depositor\|Developer	Kataoka, Takao \|

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mouse Ifng

External Database
mouse Ifng

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14519	pGL4_ifng-500-luc	DNA solution

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References and tips

Electronic file

Original reference

original	Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

Further references such as user reports and related articles (go to bottom)

Featured content	T cell receptor signaling pathway (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14519_A6Lfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert Sequence file: RDB14519_A6Lfa.seq
>D03972A5_A6Lf_1_pGL4-4174F_E08_05_ABI08.ab1 1 GTATGGGGCC AGGACTTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG 61 CCTTTCCTTG CTTTTCTGGT CATTTGCAAG AAAAGTTTGA AAAGGCTTCC CCCATTGCAT 121 GGTTTGAGAA GCCCAAGAGT TTCCTCATGG TTTGAGAAGC CCAAGAGTTT CCTTTTATTC 181 AGCCGTCCCC AACCACAAAC AAAGGCTCCC TGTGCTGTGC TCTGTGGATG AGAAATTCAC 241 ATTACAAGGG CAAAAAGGGG GAGACGTAAA AGCAATTTCC AGCCCCCACC CCAAATGGTG 301 TGAAGTAAAA GTGCTTTCAG AGAATCCCAC AAGAATGGCA CAGGTGGGCA CAGCGGGGCT 361 GTCTCATCGT CAGAGAGCCC AAGGAGTCGA AAGGAAACTC TAACATGCCA CAAAACCATA 421 GCTGTAATGC AAAGTAACTT AGCTCCCCCC ACCTATCTGT CACCATCTTA AAAAAAAAAA 481 AAAAACCAAA AAAAAACTTG TGAAAATACG TAATCCCGAG GAGCCTTCGA TCAGGTATAA 541 AACTGGAAGC CAGAGAGGTG CAGGCTATAG CTGCCATCGG CTGACCTAGA GAAGACACAT 601 CAGCTGATCC TTTGGACCCT CTGACTTGAG ACAGAAGTTC TGGGCTTCTC CTCCTGCGGC 661 CTAGCTCTGA GACAGATATC AAGATCTGGC CCTCGGCGGC CAAAGCTTGG CAAATCCGGT 721 ACTGTTGGTA AAGCCACCAT GGGAAGATGC CAAAAACATT AAGAAGGGGG CCCAACGCCA 781 TTCTACCCAC TCGAAAAACG GGACCCGCCC GGCGAGCAGC TGCACAAAAG CCATTGAAAG 841 CGCCTAACGC CCCTGGGTGC CCCGGGCACC CATCC //
Primer: SV40pro_R2 Region: Luciferase 3' Sequence file: RDB14519_A6Lfb.seq
>D03972A5_A6Lf_1_SV40pro_R2_G09_21_ABI24.ab1 1 AGTCGTGCCC TTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 61 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGAAG GTAGCTAACC AAGTTCCTCT 121 TTCAGAGGTT ATTTCAGGCC ATGGTGCTGC GCAGATCAGC GGAAGAGCGC CCAATACGCA 181 AACGGATCCT TATCGATTTT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC 241 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA 301 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 361 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 421 GCTCGAAGCG GCCGGCCGCC CCGACTCTAG ACTGCAGTTA GACGTTGATC CTGGCGCTGG 481 CGCAAGCAGC AGGGTGTCTA TCCATGCCGC TCTCCTGGGC GCAGCTCATG GGCAGGGTGC 541 CGGCGGCCTG CTCCTCCACC TCGGGAGGGA AGCCGTGGCT GTTAAGGTGG ATCACAAAGT 601 GGCTTAAGCT ACTGAACCAG TTCTTGCAAG CAGAATTCAC GGCGATCTTG CCGCCCTTCT 661 TGGCCTTAAT GAGAATCTCG CGGATCTTGC GGGCGTCCAA CTTGCCGGTC AGTCCTTTAG 721 GCACCTCGTC CACGAACACA ACACCACCGC GCAGCTTCTT GGCGGTTGTA ACCTGGCTGG 781 CCACATAGTC CACGATCTCC TTCTCGGTCA TGGTTTTACC GTGTTCCAGC ACGACGACTG 841 CGGCGGGCAG CTCGCCGGCA TCGTCGTCGG GCAGGCCGGC GACCCCGGCG TCGAAAGATG 901 TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGGCTGGG GCTACCTGGT AGCCCTTGTA 961 TTTGATCAGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT CGTCCTCGAT CCCAGTAGGC 1021 GATGTCGCCC GCTGTGCAGC CCAGCCGTCC CTTTGTCGAT GAGAGCGTTT TGTAGCCTCG 1081 GGATTGTTAA CGGTAAGCCG CTCAATGGAT CCATTGGGTC CACGGAACGC AACAAGACTT 1141 CGCCCGCGCC TGGGTTTCCA CCACACCCCG AGT //
Primer: SV40pro_F Region: SV40 pro/ori, PuroR, poly(A) signal Sequence file: RDB14519_A6Lfc.seq
>D03972A5_A6Lf_1_SV40pro_F_B09_02_ABI08.ab1 1 CCCCGGGCCT GGGGCATTCA AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 61 AAAAAGCTCG ATTCTTCTGA CACTAGCGCC ACCATGACCG AGTACAAGCC TACCGTGCGC 121 CTGGCCACTC GCGATGATGT GCCCCGCGCC GTCCGCACTC TGGCCGCCGC TTTCGCCGAC 181 TACCCCGCTA CCCGGCACAC CGTGGACCCC GACCGGCACA TCGAGCGTGT GACAGAGTTG 241 CAGGAGCTGT TCCTGACCCG CGTCGGGCTG GACATCGGCA AGGTGTGGGT AGCCGACGAC 301 GGCGCGGCCG TGGCCGTGTG GACTACCCCC GAGAGCGTTG AGGCCGGCGC CGTGTTCGCC 361 GAGATCGGCC CCCGAATGGC CGAGCTGAGC GGCAGCCGCC TGGCCGCCCA GCAGCAAATG 421 GAGGGCCTGC TTGCCCCCCA TCGTCCCAAG GAGCCTGCCT GGTTTCTGGC CACTGTAGGA 481 GTGAGCCCCG ACCACCAGGG CAAGGGCTTG GGCAGCGCCG TCGTGTTGCC CGGCGTAGAG 541 GCCGCCGAAC GCGCCGGTGT GCCCGCCTTT CTCGAAACAA GCGCACCAAG AAACCTTCCA 601 TTCTACGAGC GCCTGGGCTT CACCGTGACC GCCGATGTCG AGGTGCCCGA GGGACCTAGG 661 ACCTGGTGTA TGACACGAAA ACCTGGCGCC TAATGATCTA GAACCGGTCA TGGCCGCAAT 721 AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGTTCG AACTAGATGC 781 TGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC 841 ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG 901 CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 961 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAAGGGGATA 1021 ACGCAGGAAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 1081 GCGTGCTGGC GTTTTTCCAT AGCTCGCCCC CCTGACGAGC ATCACAAAAA TCGAACGCTC 1141 AGTCAGAGGT TGGCGGAAGC CGGACCAGGA ACTAATAAAG AAATACCAGG GGCGGTTTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl