Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFBD-Hs alpha1(K40R) CFXaGH/beta3 CFXaGF

Expression vector of His tagged human tubulin alpha-1(mutant, K40R, constitutive active form) and Flag tagged human tubulin beta-3.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013)を引用し、謝辞の表明を必要とする。本件リソースは学術機関での学術研究への利用に限る。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請を行う場合は、受託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14531_A7A5p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pPolyhedrin_F Region: polyhedrin pro, L21, insert2-5' Sequence file: RDB14531_A7A5g.seq
>D03763A2_A7A5_1_pPolyhedrin_F_F03_18_ABI24.ab1     1 CATTTTACTG TTTTCGTACA GTTTTGTAAT AAAAAAACCT ATAAATATTC CGGATTATTC    61 ATACCGTCCC ACCATCGGGC GCGGATCCCG GTCCGAAGCG CGCGGAATTC AAAGGCCTAC   121 GTCGACGAGC TCACTAGTAA CTCCTAAAAA ACCGCCACCA TGCGCGAGTG CATCTCCATC   181 CACGTGGGCC AGGCTGGTGT CCAGATCGGC AACGCTTGCT GGGAGCTGTA CTGCCTGGAG   241 CACGGTATCC AGCCCGACGG CCAGATGCCC TCCGACCGCA CCATCGGTGG CGGCGACGAC   301 TCCTTCAACA CCTTCTTCTC CGAAACCGGT GCTGGCAAGC ACGTGCCCCG CGCTGTGTTC   361 GTGGATCTGG AACCCACCGT GATCGACGAA GTGCGTACCG GCACCTACCG TCAGCTGTTC   421 CACCCCGAGC AACTGATCAC CGGCAAGGAG GACGCTGCTA ACAACTACGC TCGTGGTCAC   481 TACACCATCG GCAAGGAAAT CATCGACCTG GTCCTGGACC GTATCCGCAA GCTGGCTGAC   541 CAGTGCACCG GCCTGCAGGG ATTCCTCGTG TTCCACTCCT TCGGTGGTGG AACCGGTTCT   601 GGTTTCACCT CCCTGCTGAT GGAACGTCTG TCTGTGGACT ACGGCAAGAA GTCCAAGTTG   661 GAGTTCTCCA TCTACCCCGC TCCCCAGGTG TCCACCGCTG TGGTCGAGCC CTACAACTCC   721 ATCCTGACCA CCCACACCAC CCTGGAACAC TCCGACTGCG CTTTCATGGT GGACAACGAG   781 GCTATCTACG ACATCTGCCG TCGTAACCTG GACATCGAGC GTCCCACCTA CACCAACCTG   841 AACCGTCTGA TCTCCCAGAT CGTGTCCTCC ATCACCGCTT CCCTGCGTTT CGACGGTGCT   901 CTGAACGTGG ACCTGACCGA GTTCCAGACC AACCTGGTGC CCTACCCCCC GTATCCACTT   961 TCCCCCTGGC TACCTACGCT CCCGTGATCT CCCGCTGAGA AGGCTTTACC CACGAGGCAG  1021 CCTGGTCGTG GCTGAAGATC ACCAAACGTC TTGCCTTTCG AGCCGCTACC AGAATTGATC  1081 CAGTTGCCGA ACCCTCGGTC ACGGCAAGTT ACCATGTCTG ACATGGCTAC CTGTATATAC 1141 G //
Primer: SV40polyA-R Region: Tn7L, 8xHis, Factor Xa, insert2-3' Sequence file: RDB14531_A7A5h.seq
>D03763A2_A7A5_1_SV40polyA_R_B06_02_ABI08.ab1     1 AAAAAAGTGG GAGTTTTTAA GCAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT    61 CCTCTAGTAC TTCTCGACAA GCTTTTAGTG GTGATGGTGG TGATGATGGT GACCACCGGA   121 ACCACCACGA CCCTCGATGT ATTCCTCGCC TTCCTCTTCG CCCTCGCCCT CGACGGAGTC   181 GACGCCCACT TCCTCGTAGT CCTTTTCCAG AGCAGCCATG TCCTCGCGAG CCTCGGAGAA   241 CTCGCCCTCT TCCATGCCTT CGCCCACGTA CCAGTGCACG AAAGCGCGCT TAGCGTACAT   301 CAGGTCGAAC TTGTGGTCCA GACGAGCCCA AGCCTCAGCG ATAGCGGTGG TGTTGGACAG   361 CATGCACACA GCACGCTGCA CCTTAGCCAA GTCGCCACCG GGCACCACAG TGGGGGGCTG   421 GTAGTTGATT CCGACCTTGA AACCGGTGGG GCACCAGTCC ACGAACTGGA TGGAACGCTT   481 GGTCTTGATG GTAGCGATAG CAGCGTTCAC GTCCTTAGGC ACCACGTCGC CACGGTACAG   541 CAGGCAGCAA GCCATGTACT TGCCGTGACG GGGGTCGCAC TTGACCATCT GGTTAGCGGG   601 CTCGAAGCAG GCGTTGGTGA TCTCAGCCAC GGACAGCTGC TCGTGGTAAG CCTTCTCAGC   661 GGAGATCACG GGAGCGTAGG TAGCCAGGGG GAAGTGGATA CGGGGGTAGG GCACCAGGTT   721 GGTCTGGAAC TCGGTCAGGT CCACGTTCAG AGCACCGTCG AAACGCAGGG AAGCGGTGAT   781 GGAGGACACG ATCTGGGAGA TCAGACGGTT CAGGTTGGTG TAGGTGGGAC GCTCGATGTC   841 CAGGTTACGA CGGCAGATGT CGTAGATAGC CTCGTTGTCC ACCATGAAAG CGCAGTCGGA   901 GTGTTCCAGG GTGGTGTGGG TGGTCAGGAT GGAGTTGTAG GGCTCGACCA CAGCGGTGGA   961 CACCTGGGGA GCGGGGTAGA TGGAGAACTC CAACTTGGAC TTCTTGCCGT AGTCCACAGA  1021 CAGACGTTCA TCAGCCAGGG AGGTGAAAAC CAGAGCGGGT TCACACGCCG AGTGGAACAC  1081 GAAGAATCCT GCAAGCGTGC ACTGGTCAGC CAGCTGCGAT ACGTTCAAGA ACAAGGTCGA 1141 ATGAATTTCC TGTGCCGAG //
Primer: pP10_F Region: p10pro, L21, insert1-5' Sequence file: RDB14531_A7A5i.seq
>D03763A2_A7A5_1_pP10_F_C06_03_ABI08.ab1     1 ATTATAGGAT TATTATCAAA TCATTTGTAT ATTAATTAAA ATACTATACT GTAAATTACA    61 TTTTATTTAC AATCACTCGA CGAAGACTTG ATCACCCGGG ATCTCGAGAA CTCCTAAAAA   121 ACCGCCACCA TGCGCGAGAT CGTGCACATC CAGGCTGGTC AGTGCGGTAA CCAGATCGGT   181 GCTAAGTTCT GGGAGGTCAT CTCCGACGAG CACGGTATCG ACCCTTCCGG TAACTACGTG   241 GGTGACTCCG ACCTGCAGCT GGAGCGCATT TCCGTGTACT ACAACGAAGC CTCCTCCCAC   301 AAATACGTGC CTCGCGCTAT CCTGGTGGAC CTGGAGCCTG GTACTATGGA CTCTGTGCGC   361 TCCGGTGCTT TCGGTCACCT GTTCCGCCCT GACAACTTCA TCTTCGGTCA GTCCGGTGCT   421 GGTAACAACT GGGCTAAGGG TCACTACACC GAGGGTGCTG AGCTCGTCGA CTCCGTGCTG   481 GACGTGGTGC GCAAGGAGTG CGAGAACTGC GACTGCCTGC AGGGTTTCCA GCTGACTCAC   541 TCCCTGGGTG GTGGTACCGG ATCTGGTATG GGTACCCTGC TGATCTCCAA GGTGCGCGAG   601 GAGTACCCTG ACCGCATCAT GAATACCTTC TCCGTCGTGC CTTCCCCTAA GGTGTCCGAC   661 ACCGTGGTGG AGCCTTACAA CGCTACCCTG TCCATCCACC AACTGGTGGA GAACACCGAC   721 GAGACCTACT GCATCGACAA CGAGGCTCTG TACGACATCT GCTTCCGCAC CCTGAAGCTG   781 GCTACCCCTA CCTACGGTGA CCTGAACCAC CTGGTCTCCG CTACCATGTC CGGTGTGACC   841 ACCTCCCTGC GCTTCCCTGG TCAGCTGAAC GCTGACCTGC GCAAGCTGGC TGTGAACATG   901 GTGCCTTTCC CTCGCCTGCA CTTCTTCATG CCTGGTTTCG CTCCTCTGAC CGCTCGCGGT   961 TCCCAGCAAT ACCGCGCTCT GACCGTGCCT GAGCTGACCC AGCAGATGTT CGACGCTAAG  1021 AACATGATGG GCTGCTTGCG ACCCTCGCCA CGGTCGCTAC CTGACCGTGG CTACCGTGTT  1081 CCGCGGTCGC ATGTCATGAA GGAGGTGGAC GAGCCAGATG CCTGGCCTAT CAAGTCCAGA 1141 CTCCTCCTAC CTTTCGTGGG AGAGTTGGAA TTTC //
Primer: HSV_TK_pA_R Region: FLAG,Factor Xa,insert1-3' Sequence file: RDB14531_A7A5j.seq
>D03763A2_A7A5_1_HSV_TK_pA_R_D07_04_ABI08.ab1     1 AGCGGGATCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC ATCTCCCGGT    61 ACCGCATGCT ATGCATCAGC TGCTAGCTTA CTTGTCATCG TCATCTTTGT AGTCACCACC   121 GGAACCACCA CGACCCTCGA TCTTAGGACC CTGAGCCTCG GACTCTTCCT CATCGTCCTC   181 GTACATCTCA CCCTCTTCTT CAGCGGTAGC GTCCTGGTAC TGCTGGTACT CGGACACCAG   241 GTCGTTCATG TTGGACTCAG CCTCGGTGAA CTCCATCTCG TCCATACCCT CACCGGTGTA   301 CCAGTGCAGG AAAGCCTTGC GGCGGAACAT AGCGGTGAAC TGCTCGGAGA TGCGCTTGAA   361 CAGCTCCTGG ATAGCGGTGG AGTTACCGAT GAAGGTGGAG GACATCTTCA GACCGCGAGG   421 AGGGATGTCG CACACAGCCA CCTTCACGTT GTTAGGGATC CACTCCACGA AGTAGGAGGA   481 GTTCTTGGAC TGGATAGCCA GCATCTGCTC GTCCACCTCC TTCATGGACA TGCGACCGCG   541 GAACACGGTA GCCACGGTCA GGTAGCGACC GTGGCGAGGG TCGCAAGCAG CCATCATGTT   601 CTTAGCGTCG AACATCTGCT GGGTCAGCTC AGGCACGGTC AGAGCGCGGT ATTGCTGGGA   661 ACCGCGAGCG GTCAGAGGAG CGAAACCAGG CATGAAGAAG TGCAGGCGAG GGAAAGGCAC   721 CATGTTCACA GCCAGCTTGC GCAGGTCAGC GTTCAGCTGA CCAGGGAAGC GCAGGGAGGT   781 GGTCACACCG GACATGGTAG CGGAGACCAG GTGGTTCAGG TCACCGTAGG TAGGGGTAGC   841 CAGCTTCAGG GTGCGGAAGC AGATGTCGTA CAGAGCCTCG TTGTCGATGC AGTAGGTCTC   901 GTCGGTGTTC TCCACCAGTT GGTGGATGGA CAGGGTAGCG TTGTAAAGGC TCCACCACGG   961 TGTCGGACAC CTTAGGGAAG GCACGACGGA GAAGGTATTC ATGATGCGGT CAGGGTACTC  1021 CTCGCGCACC TTGGAGATCA GCAGGTTACC CATACCAGAT CCGGGTACCA CCACCCCAGG  1081 GAGTGAGTCA GCTGGAAACC TGCAGCAGGT CGCAGTTCTC GCCTCCTGCG CCACCACGGT 1141 TCAGGCAACG GAGTCGGAAC GGAGCCTCAA GCACCCCCCT TCCGGGG //
Primer: Pcpro_F Region: GmR, Tn7R Sequence file: RDB14531_A7A5k.seq
>D03763A2_A7A5_1_Pcpro_F_E07_05_ABI08.ab1     1 GAGACGGGAA GGGGGCTCAC GGCAACTGGG TCCAGAACCT TGACCGAACG CAGCGGTGGT    61 AACGGCGCAG TGGCGGTTTT CATGGCTTGT TATGACTGTT TTTTTGTACA GTCTATGCCT   121 CGGGCATCCA AGCAGCAAGC GCGTTACGCC GTGGGTCGAT GTTTGATGTT ATGGAGCAGC   181 AACGATGTTA CGCAGCAGCA ACGATGTTAC GCAGCAGGGC AGTCGCCCTA AAACAAAGTT   241 AGGTGGCTCA AGTATGGGCA TCATTCGCAC ATGTAGGCTC GGCCCTGACC AAGTCAAATC   301 CATGCGGGCT GCTCTTGATC TTTTCGGTCG TGAGTTCGGA GACGTAGCCA CCTACTCCCA   361 ACATCAGCCG GACTCCGATT ACCTCGGGAA CTTGCTCCGT AGTAAGACAT TCATCGCGCT   421 TGCTGCCTTC GACCAAGAAG CGGTTGTTGG CGCTCTCGCG GCTTACGTTC TGCCCAAGTT   481 TGAGCAGCCG CGTAGTGAGA TCTATATCTA TGATCTCGCA GTCTCCGGCG AGCACCGGAG   541 GCAGGGCATT GCCACCGCGC TCATCAATCT CCTCAAGCAT GAGGCCAACG CGCTTGGTGC   601 TTATGTGATC TACGTGCAAG CAGATTACGG TGACGATCCC GCAGTGGCTC TCTATACAAA   661 GTTGGGCATA CGGGAAGAAG TGATGCACTT TGATATCGAC CCAAGTACCG CCACCTAACA   721 ATTCGTTCAA GCCGAGATCG GCTTCCCGGC CGCGGAGTTG TTCGGTAAAT TGTCACAACG   781 CCGCGAATAT AGTCTTTACC ATGCCCTTGG CCACGCCCCT CTTTAATACG ACGGGCAATT   841 TGCACTTCAG AAAATGAAGA GTTTGCTTTA GCCATAACAA AAGTCCAGTA TGCTTTTTCA   901 CAGCATAACT GGACTGATTT CAGTTTACAA CTATTCTGTC TAGTTTAAGA CTTTATTGTC   961 ATAGTTTAGA TCTATTTTGT TCAGTTTAAG ACTTTATTGT CCGCCACACC CGCCTTACGC  1021 AGGGCATCCA TTTAATTACT CAAACCGTAA CCGATTTTGC CCAGGTTACG CGGCTGGTCT  1081 TATGCCGGGT GTGAATACCG CAACAGATGC GGTAAGGGAG AAAATACCGG CAATCAAGCG 1141 CCTCCTCGGC CTTTCCTCGC CTCAACTTGA ACTCTTCGCC TTGTGGCGC //
Primer: f1ori_F Region: f1ori, Tn7L, SV40p(A), 8xHis, FactorXa, insert2-3' Sequence file: RDB14531_A7A5l.seq
>D03763A2_A7A5_1_f1ori_F_H03_24_ABI24.ab1     1 CGGGGGCTAG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC    61 GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCAAA TAAGCGTTGA   121 TATTCAGTCA ATTACAAACA TTAATAACGA AGAGATGACA GAAAAATTTT CATTCTGTGA   181 CAGAGAAAAA GTAGCCGAAG ATGACGGTTT GTCACATGGA GTTGGCAGGA TGTTTGATTA   241 AAAACATAAC AGGAAGAAAA ATGCCCCGCT GTGGGCGGAC AAAATAGTTG GGAACTGGGA   301 GGGGTGGAAA TGGAGTTTTT AAGGATTATT TAGGGAAGAG TGACAAAATA GATGGGAACT   361 GGGTGTAGCG TCGTAAGCTA ATACGAAAAT TAAAAATGAC AAAATAGTTT GGAACTAGAT   421 TTCACTTATC TGGTTCGGAT CTCCTAGGCT CAAGCAGTGA TCAGATCCAG ACATGATAAG   481 ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG   541 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA   601 CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA   661 AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCCTCTAG TACTTCTCGA   721 CAAGCTTTTA GTGGTGATGG TGGTGATGAT GGTGACCACC GGAACCACCA CGACCCTCGA   781 TGTATTCCTC GCCTTCCTCT TCGCCCTCGC CCTCGACGGA GTCGACGCCC ACTTCCTCGT   841 AGTCCTTTTC CAGAGCAGCC ATGTCCTCGC GAGCCTCGGA GAACTCGCCC TCTTCCATGC   901 CTTCGCCCAC GTACCAGTGC ACGAAAGCGC GCTTAGCGTA CATCAGTCGA ACTTGTGGTC   961 CAGACGAGCC CAAGCCCTCA GCGATAGCGG TGGTGTTTGG GACAGCATGC ACACAGGCAC  1021 GGCTGCCACT TTAGCCAAGG TCGGCACCGG CATCACAGTG GGAGCCTGGT AGTTGAATTC 1081 GAACGTTGCA AACCGGATGC G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl