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delta N-PrP-EBFB

E.coli expression vector, C-terminal domain (residues 125 to 231) of mouse prion protein (PrP) linked to EBFP.

Catalog number RDB14969
Resource name delta N-PrP-EBFB
Clone info. E.coli expression vector, C-terminal domain (residues 125 to 231) of mouse prion protein (PrP) linked to EBFP. This clone used to utilized as the donor for the FRET analysis, see Fig. 1A, 1B and 1D of Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016.
Comment Expression was confirmed by the depositor with SDS-PAGE and FRET measurement.
Vector backbone pQBI 67 (Plasmid)
Size of vector backbone 6.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Prnp cDNA
Depositor|Developer Arii, Yasuhiro |
Other clones in our bank
External Database
Mouse Prnp
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016)。
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          RDB14969 delta N-PrP-EBFB Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Arii, Y., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Arii, Y., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243.

          2023.04.10

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