Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-human USP6NL

Expression vector of human ubiquitin specific peptidase 6 N-terminal like (USP6NL), fused with N-terminal EGFP, CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15025_A7Egp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 Sequence file: RDB15025_A7Ega.seq
>D04556G1_A7Eg_1_EGFP-C2_F03_18_ABI24.ab1     1 TAACCAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC    61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGAATTCAG ACCAGGATGT   121 AGCACTCAAA CTTGCCCAGG AGCGAGCTGA AATAGTTGCT AAATATGACA GAGGACGAGA   181 AGGTGCAGAG ATTGAACCTT GGGAAGATGC TGATTACCTT GTTTACAAAG TCACAGATAG   241 ATTTGGCTTT TTACATGAGG AGGAGCTCCC AGATCATAAT GTGGCTGTGG AACGGCAAAA   301 GCACCTGGAA ATTGAAAGAA CTACCAAATG GCTGAAAATG CTGAAAGGAT GGGAAAAATA   361 CAAGAACACT GAAAAGTTTC ATAGGCGAAT TTACAAAGGA ATACCACTCC AGCTCAGAGG   421 TGAAGTCTGG GCCCTCCTTC TTGAGATCCC TAAAATGAAA GAAGAAACAA GGGACCTGTA   481 TAGTAAATTA AAACACAGAG CACGGGGCTG TTCACCTGAC ATCAGACAAA TAGACCTGGA   541 TGTCAACCGC ACATTTCGGG ACCACATTAT GTTTAGAGAC AGATATGGTG TTAAGCAACA   601 ATCCTTATTC CATGTGCTTG CTGCCTATTC TATTTATAAC ACGGAAGTCG GGTATTGTCA   661 GGGGATGAGC CAGATCACAG CTTTACTCCT CATGTATATG AACGAGGAAG ATGCCTTCTG   721 GGCCCTGGTC AAACTCTTCT CAGGCCCTAA ACATGCCATG CATGGCTTTT TTGTCCAAGG   781 TTTTCCTAAA CTCTTGAGGT TTCAAGAACA TCATGAAAAA ATACTGAACA AATTTCTGTC   841 CAAGCTTAAG CAACACTTGG ATTCTCAAGA AATCTACACA AGTTTTTACA CAATGAAATG   901 GTTTTTTCAG TGTTTCCTTG ATCGTACTCC CTTTACACTA AACCTCAGAA TATGGGATAT   961 CTACATCTTT GAAGGAGAAC GAGTTTCTTT ACTGCTATGT CTTTACACCA TCTTTAAAAT  1021 TACACAAAAA AAACATCTAA ATGAAATTGT CATGCAGACT TGTAGAATTT TTTCCATGAG  1081 ACCTTACAAA GATTTTGTGA GATGATTTGT GATAGAGCCA CCTTCAGAAT TTCTTATTGA 1141 CCG //
Primer: SV40polyA-R Sequence file: RDB15025_A7Egb.seq
>D04556G1_A7Eg_1_SV40polyA-R_G03_21_ABI24.ab1     1 CAATGCAGTT TTAGCAGTAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCAGTTATC    61 TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTAC ATTCCATAAA GAGGACAGAA   121 ATGGATTGTA TAAATGGCCA CCCTGGGACC TTCGTGGGGA GAAGCCATGT GAGTCGAGGC   181 AGAAGAAAGA AGTAACCACA CTGCTAATGC AGTTAGTGTG GCTGCCCCAC ATGAGTACTG   241 TGAGCACTGA GGGATCAGTA AGCTGCAACT TCTCCTGCTC TGAGATTCGC AAGAGTCAGA   301 CCAGAACAAA GGGCACAAAC GCACTAAGAC CAATCTCTTA GTATGTGGAT GTGATAAGCA   361 CTGCTGCACC TTTGGCCGTA AACTTATTTT AAGTGCCCCG ATTTTTAAGC ACCATTAAGT   421 GGTTAGCCTT TCTCTCCAAT GTCTTTAATT TTCAGATTGA AAGAAAATGG CAACAGCTGA   481 AGGAGAATGA TGAGCATTCT GTGAGACTGC AATTAATAAA GTCAGGACAA ACTCTTCAGT   541 CAGTTTAGGA CCCAGGTTAC AAATATATGA GAGGGAAAAA GAAAAACTGG ACTAAAAGAC   601 AAGGAAAACA AGAATTACAT ATTATTCCAT GGATCTGGAT TAAAAGTAGT TTTTTAAAAA   661 ACGTTTCTTT AGCTCTGTGT TCAAATGTGG ATGAACAGAT GCCTATATCT ATTAAAAGAC   721 ACATACACTA CATATAGCCT TAAAAATATC TATTCTACAG TTTTTTCAGT ACTTTGTAAC   781 ATTAGTCAAA ATAGAATGAG CCAACAGAGT AAAAATGTTC AGGTTTTGGA GAAAAACATA   841 ACCGGAACTG AGTAATAAAT TACCACTGTG CGTGCATCCC TAAATGCTAT TGCGATGTTT   901 CCGGGTTAAA TTCTAACAGG TCAGTGATGA TGCAATTGAA ACGCTCATCT TAAGCAGCAT   961 CTACGTGGGG CTGAAGACAT TTCCCTGTAT TCTTATTACT AACAGACAGG AGAGATGTGC  1021 GAGTTTGTTT ACAATAGTAT AAATACTGCT TTGGGCAATT ATGGAACATA GCATGTAGTT  1081 TCACGTGTTT CTCTTCTCGG TCCTTTAGCC AAGTACACGT CCAAATCTCA CAGTCATCAC 1141 TGTCTCATGC ATGTGGAAC //
Primer: RDB15025#1 Sequence file: RDB15025_A7Egc.seq
>D04556G1_A7Eg_1_RDB15025#1_A12_01_ABI08.ab1     1 TCCTTTTTTG GCCAAAATTG AAACATAGCA ATGTAGGTTT CACGTGGTTT CTCTCTCGTC    61 TTTAGCAAGT ACACGTCAAA TCTCACAGCA ACACTGACTC TTGGATGGAA AGCCCGTCCC   121 GATTCCTGTA GTGGTAGGCT GGAGGCGGGG GCCCTGAATA TGGATATCCA GATGGACTGG   181 CATCTTCTGC GGCCGGTGAA GCTTTATATC TCACGGGACT ATCTACAGAA ACTGCAGGGA   241 GGCCATGGTC CTGAAAGGGT GCGAGTTGAA AGGCTGAGTA TTTTGGTAAA TTGCCACGGC   301 TAGCATCTCG GGTCCATGAT TGTCCCTGCG TCTCAGGTCT GTATGTATAA CTAACTTCTG   361 ACCATGTTCT GTTATCTGGC AAGTAATCCA CTGGTGGAAT GATCAATTTT CCATTCTTTG   421 GTGACCCTGA ATTGCCCGAA TATCCCCCAG CACCAGTGTC AACAGGGAGG ACTTCTATTC   481 GACTAGACGG CAGTACAAGG GGGCTTGGGC GGCTGTAAGA TTTCTCCGGA GAAGCACTGA   541 CGGAAAGAGT AGAACCATGA GGTCTCCTGG AAGGATTCAG TTGAGTCCCA GGGCTAAACT   601 GTGGAGAAGC AAAGCTGCTG TTGGCAGTAG GGAAGTGTTT GGGAGAGTTT CCGTGGTAAA   661 CGGGGGGATT GCTGTAGGAG GGGGGATGAG CTAGCCCTCG GGCTTCCCCA TCTAGCTGGG   721 ACGGATATCG TGCATGACTT GGAGGCTGTA CTTTAAAAGT AAAACTTGTT GGATACTTTT   781 GATGGACTAG AACTTGGCTC AGCGTGCTTT CTCGGGCTGG GAGGGTAAAG GGCATGCCGG   841 GGGGCTCTGG GAGTAAGCCC TTTCCAGCGC CTCCTCCACG GAAGCGCCGC TGTCCAGCTC   901 CGGGCCCTGG CACGTTGTCG TACTGCGATG CAGTGGAGCC CCGCTTCCCG TCCTCAGCAT   961 CCAGGGCCTT CATCTTTGGG CCGCAACGTT TGACACCCGC ACCTCGGCAG GACCTGGGAC  1021 GGGTAACTGC CGAGCGCGGG GTGCGCTGCC TCGACCCTTT GCCTTTCCAT GGAGTATTTG  1081 GCAGTTCTCT CTGTAGCTGA GACGTCTGAC GGTTTATTCC ATTTGGCACA ACTCCTCTGA 1141 TATTTGAAGT GGCGGTTGCC TATTTTTGGG TTGTGGCAGA GCCTAT //
Primer: SV40pro_F_V2 Sequence file: RDB15025_A7Egd.seq
>D04556G1_A7Eg_1_SV40pro_F_V2_H03_24_ABI24.ab1     1 GACGAGCTTT TTTGGAGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG    61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC   901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG   961 GAATCGTTTT CCGGGACGCC GGCTGGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGA  1021 GTTCTTTCGC CCACCCCTCG GGGAGGCTAA CTGAAACACG GAGAGACATA CTGCATGACC 1081 CGCGCTATGA CGGCAATTAA AGACCGTATT AAAACGCCAC GCTGTTTGGG ATCCGATTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl