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pmStr-C1-mouse Rab11A-S25N (#RDB15032)

Expression vector of mouse constitutively negative (CN) mutant Rab11A (S25N), fused with N-terminal mStr, CMV promoter.

Clone info. Expression vector of mouse constitutively negative (CN) mutant Rab11A (S25N), fused with N-terminal mStr, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pmStr-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab11a cDNA
Reference of insert sequence AB232605 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15032 pmStr-C1-mouse Rab11A-S25N DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmStr-C1-mouse Rab11A-S25N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15032).

Reference section:

Oguchi, M.E., Noguchi, K., Fukuda, M., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15032_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mStr-C_F (Pr0181)
Region: mStr 3', insert (S25N)
Sequence file: RDB15032_A7Ega.seq check
>D04556G8_A7Eg_2_mStr-C_F_H02_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT CGNGGNNNTG TACGAACGCG CCGAGGGCCG CCACTCCNCC
   61 GGCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGGGCACCCG CGACGACGAG
  121 TACGACTACC TCTTTAAAGT TGTCCTTATT GGAGATTCTG GTGTTGGAAA GAATAACCTC
  181 CTGTCTCGAT TTACTCGAAA TGAGTTTAAT CTGGAAAGCA AGAGTACCAT TGGAGTAGAG
  241 TTTGCAACAA GAAGCATCCA GGTTGATGGG AAAACAATAA AGGCACAGAT ATGGGACACA
  301 GCAGGGCAGG AGCGGTACAG GGCTATAACG TCTGCATACT ATCGTGGAGC AGTAGGTGCC
  361 TTATTGGTTT ATGACATTGC TAAGCATCTC ACATATGAAA ATGTAGAGCG ATGGCTGAAA
  421 GAACTGAGAG ATCATGCTGA TAGTAACATT GTTATCATGC TTGTGGGCAA TAAGAGTGAT
  481 TTACGTCATC TCAGGGCAGT TCCTACAGAT GAAGCAAGAG CTTTTGCAGA GAAGAATGGT
  541 TTGTCATTCA TTGAGACATC TGCTCTAGAT TCTACAAATG TTGAAGCTGC TTTTCAGACA
  601 ATTCTAACAG AGATATACCG CATTGTTTCT CAGAAGCAAA TGTCAGACAG ACGTGAAAAT
  661 GACATGTCTC CAAGCAACAA TGTGGTTCCT ATTCATGTTC CGCCCACCAC TGAGAACAAG
  721 CCAAAGGTGC AGTGCTGTCA GAACATCTAA AATCACTAGT GAATTCTGCA GTCGACGGTA
  781 CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG
  841 TAGAGGTTTT ACTTGCTTTN AAAAACCTCN NACACNTCCC CNTGANNCTG AAACATAAAA
  901 TGAATGCNAT TGNTGTTGTN NACTNGTTTA NNGNAGCTNA NNANGGTTAN NAATNAAGCN
  961 ATAGCATCNN NAANTTCACN AATAAAGCAT TTTTTTTTCN NNGNATNNNA NTNNNNNNTN
1021 GNNCNNANNN NNNNNNN
//
Primer: SV40polyA-R2 (Pr0623)
Region: insert 3'
Sequence file: RDB15032_A7Egb.seq check
>D04556G8_A7Eg_2_SV40polyA_R2_H03_24_ABI24.ab1
    1 NNNNNNNNCN NNANNNANAN GTTAACAACA ACNATTGCAT TNNTTTTATG TTTCNGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA
  181 TTCACTAGTG ATTTTAGATG TTCTGACAGC ACTGCACCTT TGGCTTGTTC TCAGTGGTGG
  241 GCGGAACATG AATAGGAACC ACATTGTTGC TTGGAGACAT GTCATTTTCA CGTCTGTCTG
  301 ACATTTGCTT CTGAGAAACA ATGCGGTATA TCTCTGTTAG AATTGTCTGA AAAGCAGCTT
  361 CAACATTTGT AGAATCTAGA GCAGATGTCT CAATGAATGA CAAACCATTC TTCTCTGCAA
  421 AAGCTCTTGC TTCATCTGTA GGAACTGCCC TGAGATGACG TAAATCACTC TTATTGCCCA
  481 CAAGCATGAT AACAATGTTA CTATCAGCAT GATCTCTCAG TTCTTTCAGC CATCGCTCTA
  541 CATTTTCATA TGTGAGATGC TTAGCAATGT CATAAACCAA TAAGGCACCT ACTGCTCCAC
  601 GATAGTATGC AGACGTTATA GCCCTGTACC GCTCCTGCCC TGCTGTGTCC CATATCTGTG
  661 CCTTTATTGT TTTCCCATCA ACCTGGATGC TTCTTGTTGC AAACTCTACT CCAATGGTAC
  721 TCTTGCTTTC CAGATTAAAC TCATTTCGAG TAAATCGAGA CAGGANGTTA TTCTTTCNNA
  781 CACCAGAATC TCCAATAANG ACAACTTTAA AGANGTAGTC GTACTCGTCG TCGCGGGTGN
  841 CCNTGGATCT GANNNCGGAC TTGTACAGCT CGNCCATGNC GCCNGNGGAG TGNCGGCCCN
  901 CGGCGCGTTC NNACAGTTCC NCGATGGNGN ANNCCNCNTT GNGGGANGNG ANGTCNNNNN
961 TGNTGCNNAN NNNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, mStr 5'
Sequence file: RDB15032_A7Egc.seq check
>D04556G8_A7Eg_2_CMV_Forward_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NNNNNNNGGG CGGTANGCGT GTACGGNGGG NGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
  121 AGCAAGGGCG AGGAGAATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCGC
  181 ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC
  241 TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC
  301 TGGGACATCC TAACCCCCAA CTTCACCTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC
  361 GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG
  421 AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG
  481 TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG
  541 AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG
  601 AAGGGCGAGA TCAAGATGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC
  661 AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAT CGTCGGCATC
  721 AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACTGTA CGAACGCGCC
  781 GANGGCCGCC ACTCCACCGG CGGCATGGNC GANCTGTACA AGTCCGGNNT CAGATNCATG
  841 GGNACCCGCG ACNACNANTA CGACTACCTC TTNNAAGTTG NNCTTNTNGG ANATTCNGGT
  901 GTNNGAAAGA ATANCCTNCN NNCTCNATTT ANNNNAANTN ANNTNNANNN NNGNNANNNN
961 NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB15032_A7Egd.seq check
>D04556G8_A7Eg_2_SV40pro_F_V2_B12_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNTTTTGN NNNNNCGATC NNGAGANAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACNG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG ANCNGGACTC TGGGGTTCGA AATGACCGAN CAAGCGACGC
  901 CNNNCCTGCC NTCNCGAGAT TTCNANTCCN CCGCCGNCNT TCTATGNNNN NTNNGGNNTC
961 NGNANTNNTT TNNCNNGGNN NNCNNGNNGG ATGATNCNTC NNNNNNNGGN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oguchi, M.E., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]