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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR1A-SYN-GFP-BGHpA(r) (#RDB15109)

Gateway(R) entry vector of GFP expression unit driven by the neuron-specific promoter (human synapsin I (SYN)).

Clone info. Gateway(R) entry vector of GFP expression unit driven by the neuron-specific promoter (human synapsin I (SYN)), bovine growth hormone polyadenylation signal (BGHpA) [reverse], Gateway entry vector.
Vector backbone pENTR1A (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Kanamycin
Gene/insert name Human SYN1 genomic DNA
Depositor|Developer Hioki, Hiroyuki |
 
Sequence (full) RDB15109zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
備考
Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15109 pENTR1A-SYN-GFP-BGHpA(r) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pENTR1A-SYN-GFP-BGHpA(r) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15109).

Reference section:

Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., Hioki, H., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15109_A7Fdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: KanR-N_R (Pr0530)
Region: KanR 5', attL2, SYN pro, EGFP 5'
Sequence file: RDB15109_A7Fda.seq check
>D04569A3-2_A7Fd_KanR-N_R_H03_24_ABI24.ab1
    1 TGGGGACATT TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAC
   61 GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC TGTTTATGTA AGCAGACAGT
  121 TTTATTGTTC ATGATGATAT ATTTTTATCT TGTGCAATGT AACATCAGAG ATTTTGAGAC
  181 ACGGGCCAGA GCTGCAGCTG GATGGCAAAT AATGATTTTA TTTTGACTGA TAGTGACCTG
  241 TTCGTTGCAA CAAATTGATA AGCAATGCTT TCTTATAATG CCAACTTTGT ACAAGAAAGC
  301 TGGGTCTAGA TATCTCGAGG TCCTGCAGAG GGCCCTGCGT ATGAGTGCAA GTGGGTTTTA
  361 GGACCAGGAT GAGGCGGGGT GGGGGTGCCT ACCTGACGAC CGACCCCGAC CCACTGGACA
  421 AGCACCCAAC CCCCATTCCC CAAATTGCGC ATCCCCTATC AGAGAGGGGG AGGGGAAACA
  481 GGATGCGGCG AGGCGCGTGC GCACTGCCAG CTTCAGCACC GCGGACAGTG CCTTCGCCCC
  541 CGCCTGGCGG CGCGCGCCAC CGCCGCCTCA GCACTGAAGG CGCGCTGACG TCACTCGCCG
  601 GTCCCCCGCA AACTCCCCTT CCCGGCCACC TTGGTCGCGT CCGCGCCGCC GCCGGCCCAG
  661 CCGGACCGCA CCACGCGAGG CGCGAGATAG GGGGGCACGG GCGCGACCAT CTGCGCTGCG
  721 GCGGACGGTA TCGATAAGCT TGATTCGAGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT
  781 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG
  841 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG
  901 CACCACCGGC AAGCTGCCCG TGCCCTGACC CACCCTCGTG ACCACCCTGA CCTACGGCGT
  961 GCAGTGCTTC AGCCCGCTAC CCCGACCACA TGAAAGCAGC ACGACTTTCT TTCCAAGTCC
 1021 GCCATGCCCG AAGGCTAACG TCAGGAGCCG CAACCATCCT TTCATCAGGA CGATGTCAAC
1081 TTACTAGACC CGGCGCCCGG AG
//
Primer: BGH_rev2 (Pr0606)
Region: BGH p(A), EGFP 3'
Sequence file: RDB15109_A7Fdb.seq check
>D04569A3-2_A7Ed_BGH_rev2_G03_21_ABI24.ab1
    1 CAAGTCGCGG TGAGTAGCAC GGGGGCGGGG CAACAACGAG ATGGCTGGCG AACTGAGGAA
   61 GGCACGGGAA TTCGATTTAC TTGTACAGCT CGTCCATGCC GAGAGTGATC CCGGCGGCGG
  121 TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG
  181 ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG ATGGGGGTGT
  241 TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG CGGATCTTGA
  301 AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG TGGCTGTTGT
  361 AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA
  421 GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT
  481 TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC ATGGCGGACT
  541 TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC ACGCCGTAGG
  601 TCAGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG CAGATGAACT
  661 TCAGGGTCAG CTTGCCGTAG GTGGCATCGC CCTCGCCCTC GCCGGACACG CTGAACTTGT
  721 GGCCGTTTAC GTCGCCGTCC AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT
  781 CGCCCTTGCT CACCATGGTG GCTCGAATCA AGCTTATCGA TACCGTCCGC CGCAGCGCAG
  841 ATGGTCGCGC CCGTGCCCCC CCTATCTCGC GCCTCGCGTG GTGCGGTCCG GCTGGGCCGG
  901 CGGCGGCGCG GACGCGACCA AAGTGGCCGG GAAGGGAGTT TGCGGGGGAC CGGCGAGTGA
  961 CGTCAGCGCG CCTTCAGTGC TGAGCGGCGG TGGCGCGCGC CGCCAGCGGG GGGCGAAGCA
 1021 CTGTCGCGTG CTGAGCTGGC CAATTGCGCA CGCGCTCGCG GCATCCTGAT CCCGTTCCCC
1081 CTCTCTGGAT TAGGGGATGG CGCCAATTAG A
//
Primer: BGHpA_F (Pr0373)
Region: BGH p(A), attL1, rrnB T1 T2 ter
Sequence file: RDB15109_A7Fdc.seq check
>D04569A3_2_A7Fd_BGHpA_F_D01_10_ABI24.ab1
    1 AGGGGGGGAA TGGGGTCATT CTATTCTGGG GGGGGTGGGG GTGGGGGCAG GGAACAGCAA
   61 GGGGGGAGGG ATTGGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGG
  121 GATCCAGTCG ACTGAATTGG TTCCTTTAAA GCCTGCTTTT TTGTACAAAG TTGGCATTAT
  181 AAAAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT
  241 CATTATTTGG GGCCCGAGCT TAAGTAACTA ACTAACAGGA AGAGTTTGTA GAAACGCAAA
  301 AAGGCCATCC GTCAGGATGG CCTTCTGCTT AGTTTGATGC CTGGCAGTTT ATGGCGGGCG
  361 TCCTGCCCGC CACCCTCCGG GCCGTTGCTT CACAACGTTC AAATCCGCTC CCGGCGGATT
  421 TGTCCTACTC AGGAGAGCGT TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTC
  481 CGACTGAGCC TTTCGTTTTA TTTGATGCCT GGCAGTTCCC TACTCTCGCT TAGTAGTTAG
  541 ACGTCCCCGA GATCCATGCT AGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
  601 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
  661 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGGCTCAAG
  721 GTCAGAGGGT GGCGAAACCC GACAGGAACT ATAAAGGAAT ACCAAGGCCG TTTCCCCCTG
  781 GGAAGCTCCC CTCGTGGCGC CTCCTCCTTG TTCCGGAACC CCTGTGCCGC CTTTTATACG
841 GAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]