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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAAV2-SYN-GFP-BGHpA

AAV2 expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)).

Catalog number RDB15113
Resource name pAAV2-SYN-GFP-BGHpA
Clone info. AAV2 expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)), bovine growth hormone polyadenylation signal (BGHpA), see Fig. 1A of Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017.
Comment Expression was confrimed by the depositor with microscopic observation.
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Ampicillin
Gene/insert name Human SYN1 genomic DNA
Depositor|Developer Hioki, Hiroyuki |
Other clones in our bank
External Database
Human SYN1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15113 pAAV2-SYN-GFP-BGHpA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15113_A7Fdp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-N (Pr0027)
          Region: EGFP 5', SYN pro, AAV2 ITR(L)
          Sequence file: RDB15113_A7Fda.seq check
          >D04569A7-2_A7Fd_EGFP-N_G01_07_ABI08.ab1
              1 GACCCGGGGT AAGCTCCTCG CCCTTGCTCA CCATGGTGGC TCGAATCAAG CTTATCGATA
             61 CCGTCCGCCG CAGCGCAGAT GGTCGCGCCC GTGCCCCCCT ATCTCGCGCC TCGCGTGGTG
            121 CGGTCCGGCT GGGCCGGCGG CGGCGCGGAC GCGACCAAGG TGGCCGGGAA GGGGAGTTTG
            181 CGGGGGACCG GCGAGTGACG TCAGCGCGCC TTCAGTGCTG AGGCGGCGGT GGCGCGCGCC
            241 GCCAGGCGGG GGCGAAGGCA CTGTCCGCGG TGCTGAAGCT GGCAGTGCGC ACGCGCCTCG
            301 CCGCATCCTG TTTCCCCTCC CCCTCTCTGA TAGGGGATGC GCAATTTGGG GAATGGGGGT
            361 TGGGTGCTTG TCCAGTGGGT CGGGGTCGGT CGTCAGGTAG GCACCCCCAC CCCGCCTCAT
            421 CCTGGTCCTA AAACCCACTT GCACTCATAC GCAGGGCCCT CTGCAGGACC TCGAGATATC
            481 TAGACCCAGC TTTCTTGTAC AAAGTGGTAC GCGTGCGGCC GCAGGAACCC CTAGTGATGG
          541 AGTTGGCCAC TCCCTCTCTG CGCGCTC
          //
          Primer: EGFP-C-rev (Pr0026)
          Region: EGFP
          Sequence file: RDB15113_A7Fdb.seq check
          >D04569A7-2_A7Fd_EGFP-C_rev_B05_05_ABI24.ab1
              1 CACCCCGATG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
             61 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
            121 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC
            181 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
            241 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
            301 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
            361 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
            421 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
            481 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC
            541 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
            601 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CTCGAATCAA
            661 GCTTATCGAT ACCGTCCGCC GCAGCGCAGA TGGTCGCGCC CGTGCCCCCC TATCTCGCGC
            721 CTCGCGTGGT GCGGTCCGGC TGGGCCGGCG GCGGCGCGGA CGCGACCAAG GTGGCCGGGA
            781 AGGGGAGTTT GCGGGGGACC GGCGAGTGAC GTCAGCGCGC CTTCAGTGCT GAGGCGGCGG
            841 TGGCGCGCGC CGCCAGGCGG GGGCGAAGGC ACTGTCCGCG GTGCTGAAGC TGGCAGTGCG
            901 CACGCGCCTC GCCGCATCCT GTTTCCCCTC CCCCTCTCTG ATAGGGATGC GCATTTGGGA
            961 AATGGGGGGG TTGGGGTGCT TGGTCCAGGT GGGGTCGGGG TCGGTCGTCA GGTAAGGCAC
           1021 CCCCCACCCC GCCTCATCCC TGGGTCCCTA TACCCCACTT TGCACCTCAT ACCGCAGGGC
           1081 CCTCTGGCAT GAACCCTTCC AGATATCTAG AACCCAGCTT CCTTGGTACA AAGTTGTTAC
           1141 GCGTGCCGGC CGCAGGATCT CCCTAATGAA TGATGGCAAC TCCCTCTTCT ATGCGCTGCG
          1201 TAC
          //
          Primer: EGFP-C (Pr0025)
          Region: EGFP 3', BGH p(A), attB1, AAV2 ITR（R）
          Sequence file: RDB15113_A7Fdc.seq check
          >D04569A7-2_A7Fd_EGFP-C_H01_08_ABI08.ab1
              1 CCAGGGCCCC TCCTCGGGGC ATGGGGACGG AGCTGTACAA AGTAAATCGA ATTCCTGTGC
             61 CTTCTAAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA
            121 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT
            181 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA
            241 GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG GGATCCAGTC GACTGAATTG
            301 GTTCCTTTAA AGCCTGCTTT TTTGTACAAA CTTGTCACGT GCGGACCGAG CGGCCGCAGG
          361 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCAA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl