Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBSIISK-hFLEX (#RDB15114)

Plasmid clone of Cre recombinase mediated flip-excision (FLEX) switch.

Clone info. Plasmid clone of flip-excision (FLEX) switch, genetic tool for specific gene expression under Cre recombinase exposure.
Vector backbone pBSIISK (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Depositor|Developer Hioki, Hiroyuki |
 
Sequence (full) RDB15114zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15114 pBSIISK-hFLEX DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBSIISK-hFLEX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15114).

Reference section:

Sohn, J., Takahashi, M., Okamoto, S., Ishida, Y., Furuta, T., Hioki, H., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15114_A7Fdp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_R3 ()
Sequence file: RDB15114_A7Fda.seq check
>D04569A8-2_A7Fd_f1ori_R3_D05_11_ABI24.ab1
    1 TCCATTCAGC TGCGCACTGT TGGGAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA
   61 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA
  121 GTCACGACGT TGTAAAACGA CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA
  181 ATTGGGTACC TTCGAAGGAT CCCCATAACT TCGTATAAAG TATCCTATAC GAAGTTATAT
  241 CAAAATAGGA AGACCAAGTC TTCACCATCG ACCCGAATTG CCAAGCATCA CCATCGACCC
  301 ATAACTTCGT ATAGCATACA TTATACGAAG TTATCTGTGA GTGAATTCGT CGACGATATC
  361 CTGCAGATAA CTTCGTATAG GATACTTTAT ACGAAGTTAT CATTGGGATT CTTCCTATTT
  421 TGATCCAAGC ATCACCATCG ACCCTCTAGT CCAGATCTCA CCATCGACCC ATAACTTCGT
  481 ATAATGTATG CTATACGAAG TTATGTCCCT CGAAGAGGTT CACGCGTGCG GCCGCGAGCT
  541 CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC
  601 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA
  661 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT
  721 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC
  781 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
  841 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
  901 TATCCACAGA ATCAGGGGAT AACGCAGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
  961 CAGGAACCGT AAAAAGCCGC GTTGCTGGCG TTTTTCCATA GCTCCGCCCC CTGACGAGCA
 1021 TCACAAAATC GACGCTCAAG TCAGAGTGGC GAAACCCGGA CAGACTATAA AGAATACCAG
 1081 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC CTCCTCCTGT TCCGACCTTG CGCTTACGAT
 1141 ACTGGTCGGC GTTCCTCCCT CGAAGCGATG GCCGCTTTCT CTCTATAGAG CTTACTAACG
1201 A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929. [link to RRC of NBRP]