Resource data sheet
DNA Bank Top

NPY-Venus/pEF-BOS (#RDB15119)

Venus fluorescent protein targeting localization in neurites. Mammalian expression.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Nagai, T., Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Venus fluorescent protein targeting localization in secretory dense-core granules of neurites (tagged with neuropeptide Y (NPY)). Mammalian expression.
Vector backbone pEF-BOS (plasmid)
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name Human NPY cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee
RDB15119 NPY-Venus/pEF-BOS DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The NPY-Venus/pEF-BOS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15119).

Reference section:

Nagai, T., Ibata, K., Park, E.S., Kubota, M., Mikoshiba, K., Miyawaki, A., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. [PubMed] [Article] [RRC of NBRP]
reference Oya, M., Amino acid taste receptor regulates insulin secretion in pancreatic β-cell line MIN6 cells. Genes Cells 16 (5): 608-616 (2011). PMID 21470345. [PubMed] [Article]
reference Grigoriev, I., Rab6 regulates transport and targeting of exocytotic carriers. Dev. Cell 13 (2): 305-314 (2007). PMID 17681140. [PubMed] [Article]
reference Tsuboi, T., Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells. J. Cell Sci., 119 (Pt 11): 2196-2203 (2006). PMID 16684812. [PubMed] [Article] [RRC of NBRP]