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cp145Venus

Venus fluorescent protein with circular permutation with the new N terminus at Tyr-145.

Catalog number RDB15121
Resource name cp145Venus
Clone info. Venus fluorescent protein with circular permutation by using GGSGG peptide linker to connect natural N and C termini. cp145Venus was given new N terminus at Tyr-145.
Vector backbone pRSETB (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
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External Database
Aequorea victoria GFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
            Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
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            提供条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by Dr. Atsushi Miyawaki or an acknowledgment to Dr. Miyawaki are requested.
            Nagai T, Yamada S, Tominaga T, Ichikawa M, Miyawaki A. (2004) Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U S A. 101:10554-10559.
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            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
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            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB15121 cp145Venus Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The cp145Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15121).

            Reference section:

            Nagai, T., Yamada, S., Tominaga, T., Ichikawa, M., Miyawaki, A., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content Fluorescent protein resources (English text)
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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Nagai, T., Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. [link to RRC of NBRP]

            2023.11.23

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