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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBS coupler 6

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Catalog number RDB15131
Resource name pBS coupler 6
Clone info. Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
Vector backbone Plasmid
Depositor|Developer Miyawaki, Atsushi |
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            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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            Order form [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15131 pBS coupler 6 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pBS coupler 6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15131).

            Reference section:

            Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15131_A7H9p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13R (Pr0160)
            Region: insert, f1 ori
            Sequence file: RDB15131_A7H9a.seq check
            >D04498B7_A7H9_1_M13R_E03_15_ABI24.ab1
                1 TGGCGGCAAT TTAAACCCCT CACTAAAAGG GGAACAAAAG CTGGGTACCG GGGCCCCCCC
               61 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT GGCGATCCGC CACCGCCAGA GCCACCTCCG
              121 CCTGAACCGC CTCCACCATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT TCTAGAGCGG
              181 CCGCCACCGC GGTGGAGCTC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG
              241 GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT
              301 GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT
              361 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGAAATTGT AAGCGTTAAT ATTTTGTTAA
              421 AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA
              481 AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA
              541 ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC
              601 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC
              661 GTAAAGCACT AAATCGGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG
              721 CCGGCGAACG TGGCGGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT
              781 GGGCAAGTGT AGCGGGTCAC GCTGCGCGTA ACCCACCAAC ACCCGGCCGC GGCTTAATGC
              841 GCCGCTACAA GGGCGCGGTC AGGTGGCACT TTTTCGGGGA AAATGTGCGC GGGAACCCCC
              901 CTATTTGGTT TATTTTTCTA AATAGATTTC AAATATGGTA TCCGCTCCAT GAGAACAATA
              961 ACCCTGGATA AATGCTTCAA TAATATTTGG AAAAAAGGAA AAGTATAGAG TAATTCCAAC
             1021 ATTTTCCGTG GTCGCCCCTC TAATGTCCCC TTTTTTGTGG CGGGCATTTA TGCCCTTCTC
             1081 TGTGTTTTGC GCTACACACC AGAAACGCTC GTGGTGAAAA AGATTAAAGA ATGCCTTGAA
            1141 GAATGCGTGG GTTGCAACGG ACAGGTGGGT GTTTACCACT GTGACATACT GATTGA
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP]

            2023.08.18

            GNP_filter3_RDBDEP_html_230702.pl