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CALR-mCherry-KDEL

Expression vector of rat Calreticulin (CALR), mCherry-ER retention signal (KDEL) tag.

Catalog number RDB15152
Resource name CALR-mCherry-KDEL
Clone info. Expression vector of rat Calreticulin (CALR) with C-terminally tagged mCherry-ER retention signal (KDEL).
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Calr cDNA
Depositor|Developer Hamachi, Itaru |
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External Database
rat Calr

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。
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          RDB15152 CALR-mCherry-KDEL DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The CALR-mCherry-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15152).

          Reference section:

          Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15152_A7Flp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB15152_A7Fla.seq check
          >D04425A1_A7Fl_2_CMV-Forward_A07_01_ABI24.ab1
              1 CGTTAACCTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGA
            121 GGCCACCATG CTCCTTTCGG TGCCGCTCCT GCTTGGCCTC CTCGGCCTGG CTGCCGCAGA
            181 CCCTGCCATC TATTTCAAAG AGCAGTTCTT GGACGGAGAT GCCTGGACCA ACCGCTGGGT
            241 CGAATCCAAA CATAAGTCTG ATTTTGGCAA ATTCGTCCTC AGTTCTGGCA AATTCTACGG
            301 GGACCAGGAG AAGGATAAAG GGTTGCAGAC AAGCCAAGAT GCCCGATTTT ACGCGCTGTC
            361 CGCCAGATTC GAACCCTTCA GCAACAAGGG CCAGACACTG GTGGTACAGT TCACCGTGAA
            421 GCATGAGCAG AATATCGACT GTGGGGGCGG CTACGTGAAG CTGTTTCCGG GTGGCTTGGA
            481 CCAGAAGGAC ATGCATGGAG ACTCAGAATA TAACATCATG TTTGGTCCGG ACATCTGCGG
            541 TCCTGGCACC AAGAAGGTTC ATGTCATCTT TAACTACAAG GGCAAGAACG TGCTGATCAA
            601 CAAGGATATC CGGTGTAAGG ATGATGAATT CACACATCTA TACACGCTGA TTGTGCGGCC
            661 AGACAACACC TACGAGGTGA AAATTGACAA CAGCCAGGTG GAGTCGGGCT CCTTGGAGGA
            721 TGATTGGGAC TTTCTGCCGC CCAAGAAGAT TAAGGATCCT GACGCTGCCA AGCCAGAAGA
            781 CTGGGATGAA CGAGCCAAGA TTGATGACCC CACAGATTCC AAGCCTGAGG ACTGGGACAA
            841 GCCAGAGCAC ATCCCTGACC CTGATGCTAA GAAGCCTGAG GACTGGGACG AAGAGATGGA
            901 TGGAGAGTGG GAACCACCAG TGATTCAAAA TCCTGAAATA CAAGGGCGAA ATGGAAGCCC
            961 ACGTCAAATT TGACACCCAG ATTACAAGGG TACCCTGCAT ACACCCAGAG ATTGAACATC
           1021 CCTGATACTC CCCCGATGCC GATATCCTAT GGCCCTAATG ATAGTTTTGC TGTACCTGAG
          1081 TCTTAGACTC TGCCGAGTCA GTCTCGCACT AAATTCATGC
          //
          Primer: mCherry-N_rev
          Sequence file: RDB15152_A7Flb.seq check
          >D04425A1_A7Fl_2_mCherry-N_R_B07_04_ABI24.ab1
              1 TACATAACTC TTGATGATGG CCATGTTATC CTCCTCGCCC TTGCTCACCA TGGTGGCGAC
             61 CGGTGGATCC CGGGCCCGCG GCAGCTCATC CTTGGCTTGG CCAGTGGCAT CCTCCTCATC
            121 TTCTTCCTTC TCATCCTCTT CATCTTCATC TTCATCTCTG TCATCCTCAT CCTCTTTATC
            181 CTCGGCCTCC TCTTCCTCTT TACGCTTCTT GTCTTCTTCT TCTTCCTTAA GCCTCTGCTC
            241 CTCATCCTGC TTGTCCTTCA TCTGCTTCTC TGCAGCCTTG GTGACACCCC AGGTCTCATT
            301 GCCAAACTCC TCTGCATAGG CCTCATCATT GGTGATGAGG AAGTTGTCAA AAATTGTGCC
            361 AGACTTGACC TGCCAGAGGT CTAAGCCCAG TACAGCAAAA CTATCATAGG CATAGATATT
            421 CGCATCGGGG GAGTATTCAG GATTGTCAAT CTCTGGGTGT ATCCAGGTAC CCTTGTAATC
            481 TGGGTTGTCA ATTTGACGTG GCTTCCATTC GCCCTTGTAT TCAGGATTTT GAATCACTGG
            541 TGGTTCCCAC TCTCCATCCA TCTCTTCGTC CCAGTCCTCA GGCTTCTTAG CATCAGGGTC
            601 AGGGATGTGC TCTGGCTTGT CCCAGTCCTC AGGCTTGGAA TCTGTGGGGT CATCAATCTT
            661 GGCTCGTTCA TCCCAGTCTT CTGGCTTGGC AGCGTCAGGA TCCTTAATCT TCTTGGGCGG
            721 CAGAAAGTCC CAATCATCCT CCAAGGAGCC CGACTCCACC TGGCTGTTGT CAATTTTCAC
            781 CTCGTAGGTG TTGTCTGGCC GCACAATCAG CGTGTATAGA TGTGTGAATT CATCATCCTT
            841 ACACCGGATA TCCTTGTTGA TCAGCACGTT CTTGCCCTTG TAGTTAAAGA TGACATGAAC
            901 CTTCTTGGTG CCAGGACCGC AGATGTCCGG ACCAAACATG ATGTTATATT CTGAGTCTCC
            961 ATGCATGTCC TTCTGGTCCA AGCCCACCCG GGAAAACAGC TTTCACGTAG CCCGCCCCCC
           1021 ACAGGTCGAT ATTCTGCTCA TGCCTCACGG TGACTGGTAC ACCAGCGTCT ATCGTGCTGC
          1081 TGAGGCTTCG ATTCTTGGAT CGGA
          //
          Primer: SV40polyA-R
          Sequence file: RDB15152_A7Flc.seq check
          >D04425A1_A7Fl_2_SV40polyA-R_C07_07_ABI24.ab1
              1 AAAAATGGGA GCTTTTTAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
             61 CTAGAGTCGC GGCCGCTAGA GCTCATCCTT CTTGTACAGC TCGTCCATGC CGCCGGTGGA
            121 GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT
            181 GATGTCCAAC TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT
            241 GTAGGTGGTC TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT
            301 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC
            361 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC
            421 CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC
            481 GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA
            541 GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA
            601 CAGGATGTCC CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG
            661 GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC
            721 GGAGCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC
            781 CTCGCCCTTG CTCACCATGG TGGCGACCGG TGGATCCCGG GCCCGCGGCA GCTCATCCTT
            841 GGCTTGGCCA GTGGCATCCT CCTCATCTTC TTCCTTCTCA TCCTCTTCAT CTTCATCTTC
            901 ATCTCTGTCA TCCTCATCCT CTTTATCCTC GGCCTCCTCT TCCTCTTTAC GCTTCTTGTC
            961 TTCTTCTTTC TTCCCTTAAA GCCTCTGCTC CTCATCCTGC TTTGTCCCTT TCATCTGCTT
           1021 CTCTGCCAGC CTTGGATGAC ACCCCCAGGT CTCATTTGGC CCAAACTCCG TCTGCATAGG
          1081 CGTCAATCAT TGTGAATGGA AGGAAGTTGT TCAAAAAAAT CG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15152_A7Fld.seq check
          >D04425A1_2_A7Fl_SV40pro_F_V2_E09_15_ABI24.ab1
              1 GGCCGGCATT TTGGAGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
             61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
            121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
            181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
            241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
            301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
            361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
            421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
            481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
            541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
            601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
            661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
            721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
            781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
            841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGGTTCGAA ATGACCGACC AAGCGACGCC
            901 CAACCCTGCC ATCACGAGAT TTCGATTCCA CCGCCCGCCC TTCTATGAAG GTTGGGGCTT
            961 CGGAATCGTT TTCCCGGGGA CGCCCGGCTG GATGATCCCT CCCAGCGCGG GGATCTCATG
           1021 CTGGGAGTTT CCTTTCGCCA ACCCTAGGGG GAGGCTTACC TGGAAACACG GATGGAGACA
          1081 ATTACCTGAG GAACCGCCGC TTATGAACCG TCCAATAAAA GGAACAGGCA T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

          2023.12.13

          GNP_filter3_RDBDEP_html_231213.pl