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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse syntaxin-2

Expression vector of mouse syntaxin-2 [Stx2 in NCBI].

Catalog number RDB15167
Resource name pEF-FLAG-mouse syntaxin-2
Clone info. Expression vector of mouse syntaxin-2 [Stx2 in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Stx2 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Stx2

          Reference sequence
            
           
          Sequence (partial) RDB15167pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 280 (47): 39175-39184, 2005)
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 280 (47): 39175-39184, 2005)。
          付加的提供条件:
          pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15167 pEF-FLAG-mouse syntaxin-2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEF-FLAG-mouse syntaxin-2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15167).

          Reference section:

          Fukuda, M., Imai, A., Nashida, T., Shimomura, H., Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J. Biol. Chem. 280 (47): 39175-39184 (2005). PMID 16186111.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15167_A7Fmp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: EF-1 alpha pro,insert 5' (FLAG,Stx2 5')
          Sequence file: RDB15167_A7Fma.seq check
          >D04709A2_1_A7Fm_EF1a-C_F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NTTTNNAGTT TGGNNCTTGG TTCNTTCTCN AGCCTCAGAC AGTGGTTCAA
             61 AGTTTTTTTC TTCCNTTTCA GGTGTCGTGA GGAATTCTCT AGAGCGGCCG CGGGAATTCG
            121 ATTCCACCAT GGACTACAAG GACGATGACG ACAAGGGATC CATGCGGGAC CGGCTGCCCG
            181 ACCTCACGGC GTGTAGGACA AACGACGATG GAGACACTGC TGTCGTCATT GTGGAGAAGG
            241 ATCATTTCAT GGACGGTTTC TTCCATCAGG TAGAGGAGAT TCGAAGCAGC ATAGCCAGGA
            301 TTGCTCAGCA TGTAGAAGAC GTGAAGAAGA ACCACAGCAT CATCCTGTCT GCTCCAAACC
            361 CAGAAGGAAA AATAAAAGAA GAGCTGGAGG ACCTGAACAA AGAGATCAAG AAAACTGCTA
            421 ACAGGATCCG GGGCAAGCTG AAGTCTATTG AGCAGAGCTG TGATCAGGAC GAGAATGGGA
            481 ACCGAACTTC AGTGGATCTG CGGATACGAA GGACCCAGCA CTCGGTGCTG TCACGGAAGT
            541 TTGTGGACGT CATGACAGAA TACAATGAAG CGCAGATCCT GTTCCGGGAG CGAAGCAAAG
            601 GCCGCATCCA GCGCCAGCTG GAGATCACTG GGAGGACCAC CACTGACGAC GAGCTGGAAG
            661 AGATGCTGGA GAGCGGGAAG CCGTCCATCT TCATCTCGGA TATTATATCA GATTCACAAA
            721 TCACTAGGCA AGCTCTCAAT GAGATCGAGT CCCGCCACAA AGACATCATG AAGCTGGAGA
            781 NCAGCATCCG AGAGCTGCAC GANATGTTCA TGGATATGGC CATGTTTGTC GAGANTCANG
            841 GTGAAATGNT CAACAACATC NAGAGAAATG TGGTGAACTC NGTANNNTAC GTGGNANNNT
            901 GCCAAGGANN ANANGAANAA AGCCATCNAA TACCANANCN NNGNCAGNNN GNAAAAAGNN
          961 NNNNNNNGNT GCTGNGGNNG NNNGNNGNCA NNNNNNNNNN
          //
          Primer: hGCSF_R (Pr0031)
          Region: hGCSF+pA,insert 3' (Stx2 3')
          Sequence file: RDB15167_A7Fmb.seq check
          >D04709A2_1_A7Fm_hGCSF-R_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GGTCNCNGGT GGGGNNCCTC ACTCTAGAGC GGCCGCTATG
             61 GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTTCAT TTGCCAACCG ACAAGCCAAT
            121 GATTAGAGCC AGGACGGCAA TGACAGCCAC CGCCACAGCA GCAATTATCC ACTTTTTCCG
            181 CCTGGCCTTG CTCTGGTATT TGATGGCTTT CTTCGTCTCT TCCTTGGCAT GTTCCACGTA
            241 ATCTACAGAG TTCACCACAT TTCTCTCGAT GTTGTTGACC ATTTCACCCT GAGTCTCGAC
            301 AAACATGGCC ATATCCATGA ACATCTCGTG CAGCTCTCGG ATGCTGGTCT CCAGCTTCAT
            361 GATGTCTTTG TGGCGGGACT CGATCTCATT GAGAGCTTGC CTAGTGATTT GTGAATCTGA
            421 TATAATATCC GAGATGAAGA TGGACGGCTT CCCGCTCTCC AGCATCTCTT CCAGCTCGTC
            481 GTCAGTGGTG GTCCTCCCAG TGATCTCCAG CTGGCGCTGG ATGCGGCCTT TGCTTCGCTC
            541 CCGGAACAGG ATCTGCGCTT CATTGTATTC TGTCATGACG TCCACAAACT TCCGTGACAG
            601 CACCGAGTGC TGGGTCCTTC GTATCCGCAG ATCCACTGAA GTTCGGTTCC CATTCTCGTC
            661 CTGATCACAG CTCTGCTCAA TAGACTTCAG CTTGCCCCGG ATCCTGTTAG CAGTTTTCTT
            721 GATCTCTTTG TTCAGGTCCT CCAGCTCTTC TTTTATTTTT CCTTCTGGGT TTGGAGCAGA
            781 CAGGANGANG CTGTGGNTCT TCTTCACGTC TTCTACATGC TGAGCAATCC TGGCTATGCT
            841 GCTTCNAATC TCCTCTACCT GATGNAAGAA ACNGTCCNTG AAATGATNCT TCNNNNCAAT
            901 GANGANAGCA NNGTCTNCNT CGNCGTTTGT CCTACACGCC NNGANGNCNG GCANCNNGNC
          961 CNNCATGGNT NCNNNGNCGN CATCGTNCNN GNANNNNNTN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Fukuda, M., Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J. Biol. Chem. 280 (47): 39175-39184 (2005). PMID 16186111.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl