RDB15167 - pEF-FLAG-mouse syntaxin-2

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icon below as well as clone information before placing your order.

pEF-FLAG-mouse syntaxin-2 (#RDB15167)

Expression vector of mouse syntaxin-2 [Stx2 in NCBI].

Clone info.	Expression vector of mouse syntaxin-2 [Stx2 in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Comment	Expression was confirmed by the depositor with immunoprecipitation and immunoblotting.
Vector backbone	pEF-BOS FLAG (Plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	mouse Stx2 cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

Sequence (partial)	RDB15167pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 280 (47): 39175-39184, 2005)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 280 (47): 39175-39184, 2005) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 280 (47): 39175-39184, 2005)。 MTAに書く付加的使用条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 280 (47): 39175-39184, 2005)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15167	pEF-FLAG-mouse syntaxin-2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse syntaxin-2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15167).

Reference section:

Fukuda, M., Imai, A., Nashida, T., Shimomura, H., Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J. Biol. Chem. 280 (47): 39175-39184 (2005). PMID 16186111. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15167_A7Fmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5' (FLAG,Stx2 5') Sequence file: RDB15167_A7Fma.seq
>D04709A2_1_A7Fm_EF1a-C_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NTTTNNAGTT TGGNNCTTGG TTCNTTCTCN AGCCTCAGAC AGTGGTTCAA 61 AGTTTTTTTC TTCCNTTTCA GGTGTCGTGA GGAATTCTCT AGAGCGGCCG CGGGAATTCG 121 ATTCCACCAT GGACTACAAG GACGATGACG ACAAGGGATC CATGCGGGAC CGGCTGCCCG 181 ACCTCACGGC GTGTAGGACA AACGACGATG GAGACACTGC TGTCGTCATT GTGGAGAAGG 241 ATCATTTCAT GGACGGTTTC TTCCATCAGG TAGAGGAGAT TCGAAGCAGC ATAGCCAGGA 301 TTGCTCAGCA TGTAGAAGAC GTGAAGAAGA ACCACAGCAT CATCCTGTCT GCTCCAAACC 361 CAGAAGGAAA AATAAAAGAA GAGCTGGAGG ACCTGAACAA AGAGATCAAG AAAACTGCTA 421 ACAGGATCCG GGGCAAGCTG AAGTCTATTG AGCAGAGCTG TGATCAGGAC GAGAATGGGA 481 ACCGAACTTC AGTGGATCTG CGGATACGAA GGACCCAGCA CTCGGTGCTG TCACGGAAGT 541 TTGTGGACGT CATGACAGAA TACAATGAAG CGCAGATCCT GTTCCGGGAG CGAAGCAAAG 601 GCCGCATCCA GCGCCAGCTG GAGATCACTG GGAGGACCAC CACTGACGAC GAGCTGGAAG 661 AGATGCTGGA GAGCGGGAAG CCGTCCATCT TCATCTCGGA TATTATATCA GATTCACAAA 721 TCACTAGGCA AGCTCTCAAT GAGATCGAGT CCCGCCACAA AGACATCATG AAGCTGGAGA 781 NCAGCATCCG AGAGCTGCAC GANATGTTCA TGGATATGGC CATGTTTGTC GAGANTCANG 841 GTGAAATGNT CAACAACATC NAGAGAAATG TGGTGAACTC NGTANNNTAC GTGGNANNNT 901 GCCAAGGANN ANANGAANAA AGCCATCNAA TACCANANCN NNGNCAGNNN GNAAAAAGNN 961 NNNNNNNGNT GCTGNGGNNG NNNGNNGNCA NNNNNNNNNN //
Primer: hGCSF_R (Pr0031) Region: hGCSF+pA,insert 3' (Stx2 3') Sequence file: RDB15167_A7Fmb.seq
>D04709A2_1_A7Fm_hGCSF-R_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGTCNCNGGT GGGGNNCCTC ACTCTAGAGC GGCCGCTATG 61 GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTTCAT TTGCCAACCG ACAAGCCAAT 121 GATTAGAGCC AGGACGGCAA TGACAGCCAC CGCCACAGCA GCAATTATCC ACTTTTTCCG 181 CCTGGCCTTG CTCTGGTATT TGATGGCTTT CTTCGTCTCT TCCTTGGCAT GTTCCACGTA 241 ATCTACAGAG TTCACCACAT TTCTCTCGAT GTTGTTGACC ATTTCACCCT GAGTCTCGAC 301 AAACATGGCC ATATCCATGA ACATCTCGTG CAGCTCTCGG ATGCTGGTCT CCAGCTTCAT 361 GATGTCTTTG TGGCGGGACT CGATCTCATT GAGAGCTTGC CTAGTGATTT GTGAATCTGA 421 TATAATATCC GAGATGAAGA TGGACGGCTT CCCGCTCTCC AGCATCTCTT CCAGCTCGTC 481 GTCAGTGGTG GTCCTCCCAG TGATCTCCAG CTGGCGCTGG ATGCGGCCTT TGCTTCGCTC 541 CCGGAACAGG ATCTGCGCTT CATTGTATTC TGTCATGACG TCCACAAACT TCCGTGACAG 601 CACCGAGTGC TGGGTCCTTC GTATCCGCAG ATCCACTGAA GTTCGGTTCC CATTCTCGTC 661 CTGATCACAG CTCTGCTCAA TAGACTTCAG CTTGCCCCGG ATCCTGTTAG CAGTTTTCTT 721 GATCTCTTTG TTCAGGTCCT CCAGCTCTTC TTTTATTTTT CCTTCTGGGT TTGGAGCAGA 781 CAGGANGANG CTGTGGNTCT TCTTCACGTC TTCTACATGC TGAGCAATCC TGGCTATGCT 841 GCTTCNAATC TCCTCTACCT GATGNAAGAA ACNGTCCNTG AAATGATNCT TCNNNNCAAT 901 GANGANAGCA NNGTCTNCNT CGNCGTTTGT CCTACACGCC NNGANGNCNG GCANCNNGNC 961 CNNCATGGNT NCNNNGNCGN CATCGTNCNN GNANNNNNTN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fukuda, M., Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J. Biol. Chem. 280 (47): 39175-39184 (2005). PMID 16186111. [link to RRC of NBRP]

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Other resources in our bank

mouse Stx2

External Database
mouse Stx2

Reference sequence