Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFN18A-HP1a-Chromo-Halo

Expression vector of Drosophila Heterochromatin protein 1a (HP1a), Chromo domain

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Vo, N., Nucleic Acids Res. 45 (3): 1233-1254, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15211_A8Ksp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Halo-C_F Sequence file: RDB15211_A8Ksa.seq
>D04544B1_A8Ks_1_Halo-C_F_F05_17_ABI24.ab1     1 GGGCGGTGAC CTGCTGGCAG GAGACAACCC GGACCTGATC GGCAGCGAGA TCGCGCGCTG    61 GCTGTCGACG CTCGAGATTT CCGGCGAGCC AACCACTGAG GATCTGTACT TTCAGAGCGA   121 TAACGCGATC GCCTACGCCG TGGAAAAGAT CATCGACAGG CGGGTGCGCA AGGGAAAGGT   181 GGAGTACTAT CTGAAATGGA AGGGCTATCC CGAAACTGAG AACACGTGGG AGCCGGAGAA   241 CAATCTCGAC TGCCAGGATC TTATCCAGCA GTACGAGGCG GTTTAAACGA ATTCGGGCTC   301 GGTACCCGGG GATCCTCTAG AGTCGACCTG CAGGCATGCA AGCTGATCCG GCTGCTAACA   361 AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC   421 TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGTT   481 CGCTTGCTGT CCATAAAACC GCCCAGTCTA GCTATCGCCA TGTAAGCCCA CTGCAAGCTA   541 CCTGCTTTCT CTTTGCGCTT GCGTTTTCCC TTGTCCAGAT AGCCCAGTAG CTGACATTCA   601 TCCGGGGTCA GCACCGTTTC TGCGGACTGG CTTTCTACGT AATGGTTTCT TAGACGTCAG   661 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT   721 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA   781 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT   841 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT   901 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT   961 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTCAA AGTTCTGCTA TGTGGCGCGG  1021 GTAATTATCC CGTATTTGAC GCCGGGGCAA GAGCAACTCG GTCGCCGCAT ACACTAATTC  1081 TCAGATGACT TTGGTTGAGT ACTCACAGTT CACAGGAAAG CATCTTACTG GATGGTCAAT 1141 GACAAGTTAA GAAGAAATTA TATTG //
Primer: rrnBT1ter_F Sequence file: RDB15211_A8Ksb.seq
>D04544B1_A8Ks_1_rrnBT1ter_F_D01_04_ABI08.ab1     1 AGGGGGCGAG GATTGACGGT TGCGAAGCAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC    61 CATAAACTGC CAGGCATCAA ATTAAGCAGA AGGCCATCCT GACGGATGGC CTTTTTGCGT   121 TTCTACAAAC TCTTTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGGAT   181 CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGTATT TTAACTTTAC TAAGGAGAAT   241 TCATCATGGC AGAAATCGGT ACTGGCTTTC CATTCGACCC CCATTATGTG GAAGTCCTGG   301 GCGAGCGCAT GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG CTGTTCCTGC   361 ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT GCACCGACCC   421 ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC CGACAAACCA GACCTGGGTT   481 ATTTCTTCGA CGACCACGTC CGCTTCATGG ATGCCTTCAT CGAAGCCCTG GGTCTGGAAG   541 AGGTCGTCCT GGTCATTCAC GACTGGGGCT CCGCTCTGGG TTTCCACTGG GCCAAGCGCA   601 ATCCAGAGCG CGTCAAAGGT ATTGCATTTA TGGAGTTCAT CCGCCCTATC CCGACCTGGG   661 ACGAATGGCC AGAATTTGCC CGCGAGACCT TCCAGGCCTT CCGCACCACC GACGTCGGCC   721 GCAAGCTGAT CATCGATCAG AACGTTTTTA TCGAGGGTAC GCTGCCGATG GGTGTCGTCC   781 GCCCGCTGAC TGAAGTCGAG ATGGACCATT ACCGCGAGCC GTTCCTGAAT CCTGTTGACC   841 GCGAGCCACT GTGGCGCTTC CCAAACGAGC TGCCAATCGC CGGTGAGCCA GCGAACATCG   901 TCGCGCTGGT CGAAGAAATA CATGGAACTG GGCTGCACCA GTCCCCTGTC CCGAAGCTGC   961 TGTTTCTGGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC CGAAGCCCGC TCGCTGGACC  1021 AAAAGCCCTG CCTAACCTGC AAGGCTGTGG AACATCGGAC CGGGATCTGA ATCTGCCTGC  1081 CAAGAGAACC ACCCTGGACC TGATCGGCAG CGAGAATCGC GCGGCGCCTG GGGCCTGATT 1141 CCGAATG //
Primer: pTrcHis-R Sequence file: RDB15211_A8Ksc.seq
>D04544B1_A8Ks_1_pTrcHiS-R_G05_20_ABI24.ab1     1 AAGGCGGAAG ATCTCGATCC TCTACGCCGG ACGCATCGTG GCCGGAGTAC TGCGGTTTAT    61 CTCGATGGCT ACGAGGGCAG ACAGTAAGTG GATTTACCAT AATCCCTTAA TTGTACGCAC   121 CGCTAAAACG CGTTCAGCGC GATCACGGCA GCAGACAGGT AAAAATGGCA ACAAACCACC   181 CGAAAAACTG CCGCAATCGC GCCTGATAAA TTTTAACCGT ATGAATACCT ATGCAACCAG   241 AGGGTACAGG CCACATTACC CCCACTTAAT CCACTGAAGC TGCCATTTTT CATGGTTTCA   301 CCATCCCAGC GAAGGGCCAT CCAGCGTGCG TTCCTGTATT TCCGGACTAT AAAAGGATCT   361 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC   421 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAAATCCT TTTTTTCTGC   481 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG   541 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA   601 ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC   661 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT   721 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA   781 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC   841 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC   901 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGGAAACGCC   961 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTTGTG  1021 ATGCTCGTCA GGGGGGCGGG AGCCTATGGG AAAAACGCCA GCCAACGCGG GCCTTTTTTA  1081 CGGATCTGAC TTTTGCTGAC TTTTGGCTCA CATGATCTTC TTGCCGATAT CCCCTTGATT 1141 TCTGGGTGGA ATACTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl