Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

GEPRA-B/ pT2KXIGdeltain

Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.

Catalog number RDB15252
Resource name GEPRA-B/ pT2KXIGdeltain
Clone info. Expression vector of genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta. For generation of transgenic zebrafish.
Vector backbone pT2KXIG deltain (plasmid)
Size of vector backbone 8.1 kb
Selectable markers Amp^r
Gene/insert name mouse RARbeta cDNA Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
mouse RARbeta Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions:
          The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Shimozono S, Iimura T, Kitaguchi T, Higashijima S, Miyawaki A. (2013) Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          5. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件:
          Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15252 GEPRA-B/ pT2KXIGdeltain DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Sensor and Visualization (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15252_A7H9p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F2
          Sequence file: RDB15252_A7H9a.seq check
          >D04498G1-2_A7H9_EF1a-C_F2_C10_07_ABI24.ab1
              1 GATGGTGGTT AGGCGGTTCG CTCTCTTCCT CACCGGGTCT GCGGCGAGTT CTAGCTGAAG
             61 CTTCCTGCAG GTCGACCGAT CCTGAGAACT TCAGGGTGAG TTTGGGGACC CTTGATTGTT
            121 CTTTCTTTTT CGCTATTGTA AAATTCATGT TATATGGAGG GGGCAAAGTT TTCAGGGTGT
            181 TGTTTAGAAT GGGAAGATGT CCCTTGTATC ACCATGGACC CTCATGATAA TTTTGTTTCT
            241 TTCACTTTCT ACTCTGTTGA CAACCATTGT CTCCTCTTAT TTTCTTTTCA TTTTCTGTAA
            301 CTTTTTCGTT AAACTTTAGC TTGCATTTGT AACGAATTTT TAAATTCACT TTTGTTTATT
            361 TGTCAGATTG TAAGTACTTT CTCTAATCAC TTTTTTTTCA AGGCAATCAG GGTATATTAT
            421 ATTGTACTTC AGCACAGTTT TAGAGAACAA TTGTTATAAT TAAATGATAA GGTAGAATAT
            481 TTCTGCATAT AAATTCTGGC TGGCGTGGAA ATATTCTTAT TGGTAGAAAC AACTACACCC
            541 TGGTCATCAT CCTGCCTTTC TCTTTATGGT TACAATGATA TACACTGTTT GAGATGAGGA
            601 TAAAATACTC TGAGTCCAAA CCGGGCCCCT CTGCTAACCA TGTTCATGCC TTCTTCTCTT
            661 TCCTACAGCT CCTGGGCAAC GTGCTGGTTG TTGTGCTGTC TCATCATTTT GGCAAAGAAT
            721 TCCTCGACGG ATCCCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
            781 CTGGTCGAGC TGCAGGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG
            841 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
            901 GTGCCCTGGC CCACCCTCGT GACCACCCTG AGCTGGGGCG TGCAGTGCTT CAGCCCGCTA
            961 CCCCGACCAC ATGAAGCAGC ACGACTTTCT TCAAGTCCGT CATGCCCGAA TCTACGTCCA
           1021 GGAGCGCACC AATCCTTCCT CCATGAACGA CGGCACTACA AGAACCGCCG CGAGTGAGTT
          1081 TCGGAGGCGA CTCCTGGTGA CGCAATCGAG CCTGAAGGAC CATCGAACCT TTCAG
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB15252_A7H9b.seq check
          >D04498G1_2_A7H9_pAxCALNLF1_A01_01_ABI08.ab1
              1 CATTAAGTAT CTTATCATGT CTGGATCATC ATCGATGAAT TAATTCGAGC TCCACCGCGG
             61 TGGCGGCCGC TCTAGAGGCT CGAGTTACTT GTACAGCTCG TTCATGCCTT CAGTGATCCC
            121 GGCGGCGGTC AGGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGAA
            181 CAGGGCGGAC TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT
            241 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG
            301 GATCTTGAAG TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG
            361 GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT
            421 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT
            481 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT
            541 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG
            601 GCCGTAGCCC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA
            661 GAGCAGCTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT
            721 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA
            781 CAGCTCCTCG CCCTTGCTCA CCATGAGCTC TTCATGTCCT TCAGAATTCT CCAGCATTTC
            841 CTGAATGAGA GGTGGCATTG ATCCAGGAAT TTCCATTTTC AAGGTAATTA CACGTTCGGC
            901 ACCTTTCGCG CTGATGCTGC GGAGATCTGT GATTTTCATT AAGATCTTTG GAAACATGTG
            961 AGGCTTGCTG GGTCGTCGTT TTCTAATGTA ATCTTTAGTG CTTCCAGCAG TGGTTCTTGG
           1021 AGCTTGTCTA CTTTTGTTGG GTTCCTCAAG GTCCTGGCGG TCTCCACAGA TTAAACAGGA
           1081 TGGCACTGAG GAGGCTGGTT CTGTGTCAAT CAATTTCCAG GCAAGAGCTT GGATGGCCAA
          1141 GTTGAACACA AAGGTCAAGT TCAGAGAGGA ACGGA
          //
          Primer: RARbeta_LBD
          Sequence file: RDB15252_A7H9c.seq check
          >D04498G1_2_A7H9_RARbeta_LBD_C01_03_ABI08.ab1
              1 CGTAATGAAG GGGTGGGCAT TGATCCAGGA TTTCCATTTT CAAGGTAATT ACACGTTCGG
             61 CACCTTTCGC GCTGATGCTG CGGAGATCTG TGATTTTCAT TAAGATCTTT GGAAACATGT
            121 GAGGCTTGCT GGGTCGTCGT TTTCTAATGT AAATCTTTAG TGCTTCCAGC AGTGGTTCTT
            181 GGAGCTTGTC TACTTTTGTT GGTTCCTCAA GGTCCTGGCG GTCTCCACAG ATTAAACAGA
            241 TGGCACTGAG AAGGCCTGTT TCTGTGTCAT CCATTTCCAA AGGCAGGAGC TGGTTGGCAA
            301 AGGTGAACAC AAGGTCAGTC AGAGGACCGA AGCCAGCATT GTGCATCTGA GTTCGATTTA
            361 GTGTAAGGCC ATCAGAGAAA GTCATGGTGT CTTGCTCTGG GGTATACCTG GTACAAATTC
            421 TGAGAATCAA GATATCCAAG CAGGCGGCTT TGAGCAGGGT GATCTGGTCT GCGATGGTCA
            481 GACCTGTGAA GCCCGGCAGA CGCTTGGCGA ACTCCACGAT CTTAATAATG CACTTGGTGG
            541 CCAGCTCACT GAATTTGTCC CAGAGGCCCA AGTCCAATCG GACCCGGTGG TCAGCGCTGG
            601 AATTCGTGGT GTATTTACCC AGCTGGCAGA GTGAGGGAAA GGTTTCCTGG TGGGCTTTCC
            661 GGATCTTCTC AGTGAGGTCG TCTAGCTCCG CTGTCATCTC ATAGCTCTCA TGCATGCGGG
            721 CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA
            781 GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGGTT CACGGGGCCG TCGCCGATGG
            841 GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCACGATG TTGTGGCGGA
            901 TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC GGTGATATAG ACGTTGTGGC
            961 TGATGTAGTT GTACTCCAGC TTTGTGCCCC AGGATGTTGC CGTCCTCCCT TGAAAGTCGA
           1021 TGCCCTTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCTC GAACTTTCAC TCCGGCGCGG
           1081 GTTCTTGTAG TTGCCGTCGT CCATGAGAGG ATGGATGCGC TTCCTGGACG GTAGGCCTTT
          1141 CGGGGCCAAG TGGAACCG
          //
          Primer: EF1a-N_R2
          Sequence file: RDB15252_A7H9d.seq check
          >D04498G1-2_A7H9_EF1a-N_R2_E10_13_ABI24.ab1
              1 GGACGGGGCA AAGGAATTGC AAGTTTGATT TGCATTGGAA AGGGTCGCTG GCTTTTGTGT
             61 TACACGCCCC TTATTTGTGC TTGATTAGAT GATCCCCCTG CTCGAGGGCC CATCTGGCCT
            121 GTGTTTCAGA CACCAGGGAG TCTCTGCTCA CGTTTCCTGC TATTTGCAGC CTCTCTATCA
            181 AGACTAATAC ACCTCTTCCC GCATCGGCTG CCTGTGAGAG GCTTTTCAGC ACTGCAGGAT
            241 TGCTTTTCAG CCCCAAAAGA GCTAGGCTTG ACACTAACAA TTTTGAGAAT CAGCTTCTAC
            301 TGAAGTTAAA TCTGAGGTTT TACAACTTTG AGTAGCGTGT ACTGGCATTA GATTGTCTGT
            361 CTTATAGTTT GATAATTAAA TACAAACAGT TCTAAAGCAG GATAAAACCT TGTATGCATT
            421 TCATTTAATG TTTTTTGAGA TTAAAAGCTT AAACAAGAAT CTCTAGTTTT CTTTCTTGCT
            481 TTTACTTTTA CTTCCTTAAT ACTCAAGTAC AATTTTAATG GAGTACTTTT TTACTTTTAC
            541 TCAAGTAAGA TTCTAGCCAG ATACTTTTAC TTTTAATTGA GTAAAATTTT CCCTAAGTAC
            601 TTGTACTTTC ACTTGAGTAA AATTTTTGAG TACTTTTTAC ACCTCTGTCA AGAACTCCTG
            661 GACAAACCTC TGACCTGTGT GGAACAGAGT GGATATGGGT GTCTGAACAG ATATTCACGT
            721 CTTTTGCAGA TCAGAGGGCA TTTCTGGTGT TGTGCAATGA CCTGGGTCCA ACAGGTAGGC
            781 GTACTCGTAT CCAAGAGCAT TGTTGGACAG GAGGTAGTCT CCATTCCTAT AAAGAGGAAG
            841 GAATGGCACC ATGTAGTAGC CGTCATTGTG GCCAATTGGT GCATTGGTAC GTGGGTAGAT
            901 GGACCGGGGA GGCTGATGAG TTCTTAGCCA GCGCTCAAGA TGCTAGAAAT GATGTAAGAA
            961 ACAATATGCA AGATGAAAGT TTAAGATAAG ATAAAAAAAT TTAACAAATA AATGCAAATT
           1021 TATGTTTTGT GTTCTATTCC ACCAATAATA AACGTCAACG CAGTAAGCCA CCAGAATATT
          1081 TTTTGGTCGT TGGCTTCTGC CAAGGCAAAC CGAGACCTCC TTAAGCA
          //
          Primer: pT2KXIG#1
          Sequence file: RDB15252_A7H9e.seq check
          >D04498G1_2_A7H9_pT2KXIG#1_B01_02_ABI08.ab1
              1 TCGGGCAAAT TAACCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG
             61 CTCTAGAACT AGTGGATCTG CTGGGCTTGC TGAAGGTAGG GGGTCAAGAA CCAGAGGTGT
            121 AAAGTACTTG AGTAATTTTA CTTGATTACT GTACTTAAGT ATTATTTTTG GGGATTTTTA
            181 CTTTACTTGA GTACAATTAA AAATCAATAC TTTTACTTTT ACTTAATTAC ATTTTTTTAG
            241 AAAAAAAAGT ACTTTTTACT CCTTACAATT TTATTTACAG TCAAAAAGTA CTTATTTTTT
            301 GGAGATCACT TCATTCTATT TTCCCTTGCT ATTACCAAAC CAATTGAATT GCGCTGATGC
            361 CCAGTTTAAT TTAAATGTTA TTTATTCTGC CTATGAAAAT CGTTTTCACA TTATATGAAA
            421 TTGGTCAGAC ATGTTCATTG GTCCTTTGGA AGTGACGTCA TGTCACATCT ATTACCACAA
            481 TGCACAGCAC CTTGACCTGG AAATTAGGGA AATTATAACA GTCAATCAGT GGAAGAAAAT
            541 GGAGGAAGTA TGTGATTCAT CAGCAGCTGC GAGCAGCACA GTCCAAAATC AGCCACAGGA
            601 TCAAGAGCAC CCGTGGCCGT ATCTTCGCAG ATCTGATCTA GAGGATCATA ATCAGCCATA
            661 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
            721 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA
            781 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT
            841 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCATCATC GATGAATTAA
            901 TTCGAGCTCC ACCGCGGTGG CGGCCGCTCT AGAGGCTCGA GTTACTTGTA CAGCTCGTTC
            961 ATGCCTTCAG TGATCCCGGC GGCGGTCAGG AACTCCAGCA GGACCATGTG ATCGCGCTTT
           1021 CTCGTTGGGG GTCTTTTGAA CAGGGCGGGA CTGGGTAGCT CAAGTAGTGG CTTGTCGGGG
           1081 CAGCAGCACG GGGTCGTCGC GATGGGGGGT GTTCCTGCTG TAGTGATCGC GAGCTGCACG
          1141 CCGGTCGGTT CCTTCGAATG TTGTGTTGGT CG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shimozono, S., Visualization of an endogenous retinoic acid gradient across embryonic development. Nature 496 (7445): 363-366 (2013). PMID 23563268.
          review Schilling, T.F., Visualizing retinoic acid morphogen gradients. Methods Cell Biol. 133: 139-163 (2016). PMID 27263412.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl