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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse myosin Vb-tail

Expression vector of mouse myosin Vb tail region (Myo5b).

Catalog number RDB15286
Resource name pEF-FLAG-mouse myosin Vb-tail
Clone info. Expression vector of mouse myosin Vb tail region [Myo5b in NCBI], tagged with N-terminal FLAG, EF1 promoter. cDNA fragment 3853nt to 5619nt of NM_201600.2 is cloned.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least) : G to A substitution at 4419nt of NM_201600.2 (Arg, Synonym).
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Myo5b cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Myo5b

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Swiatecka-Urban, A. et al., J. Biol. Chem. 282 (32): 23725-23736, 2007).
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Swiatecka-Urban, A. et al., J. Biol. Chem. 282 (32): 23725-23736, 2007)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15286 pEF-FLAG-mouse myosin Vb-tail DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Swiatecka-Urban, A., Myosin Vb is required for trafficking of the cystic fibrosis transmembrane conductance regulator in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells. J. Biol. Chem. 282 (32): 23725-23736 (2007). PMID 17462998.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15286_A7Gop1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB15286_A7Goa.seq check
          >D04773A4_A7Go_1_EF1a-C_F_D07_10_ABI24.ab1
              1 CCATTAATGA CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
             61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC
            121 GATTCCACCA TGGACTACAA GGACGATGAC GACAAGGGAT CCAGCTCCCC AGACAGCTAC
            181 AGCCTCCTCC TGAACCAGCT CAAGCTGGCC AATGAGGAGC TCGAGGTCCG CAAAGAGGAG
            241 GTGCTGATCC TCAGGACCCA GATCATGAAT GCCGACCAGC GCCGCCTGTC TGGCAAGAAC
            301 ATGGAGCCGA ACATCAATGC CAGAACAAGT TGGCCCAACA GTGAGAAGCA CGTGGACCAG
            361 GAAGACGCCA TTGAGGCCTA TCACGGGGTC TGCCAGACAA ACAGGTTGCT GGAGGCCCAG
            421 CTGCAGGCCC AGAGCCTGGA GCATGAGGAG GAGGTGGAAC ATCTCAAGGC CCAGGTGGAA
            481 GCCCTGAAAG AGGAGATGGA CAAACAGCAG CAGACCTTCT GCCAGACCCT GCTGCTCTCC
            541 CCAGAGGCCC AGGTAGAATT TGGTGTCCAG CAGGAGATAT CCCGGCTGAC CAATGAGAAC
            601 CTGGATTTTA AGGAATTGGT GGAAAAGCTG GAGAAGAATG AGAGGAAGCT GAAGAAGCAG
            661 CTGAAGATTT ACATGAAGAA GGTCCAGGAC TTAGAAGCTG CCCAGGCGTT GGCACAGAGT
            721 GACAGGAGGC ACCATGAACT CACAAGACAG GTCACAGTCC AACGAAAAGA GAAGGACTTC
            781 CAAGGCATGC TGGAGTACCA CAAAGAGGAC GAAGCCCTCC TCATCCGGAA CCTGGTGACA
            841 GACCTGAAGC CTCAGATGCT GTCGGGCACC GTGCCCTGTC TGCCTGCATA CATACTCTAT
            901 ATGTGCATCA CGCACGCGGA TTACACCAAC GATGACCTCA AAGGTGCACT CGTTGCTGAG
            961 CTCCACCATC AACGGCATTA AGAAAGTCCT CAAGAAGCAC AATGATGACT TTGAGATGAA
           1021 CGTCAATTCT GGCTATCCCA ACACTTGGCG GCTCGTCACT GTCTGAAGCA TTACAGTGAT
          1081 TGAATGAGGG ATTTTCAATG GAACACAG
          //
          Primer: hCGSF_R
          Sequence file: RDB15286_A7Gob.seq check
          >D04773A4_A7Go_1_hGCSF_R_D08_11_ABI24.ab1
              1 CTGCTCTCAT CTCTCAGGGT CCCAGGTGGG GACCCTCACT CTAGAGCGGC CGCTATGGTC
             61 GACCTGCAGG CGGCCGCGAA TTCACTAGTG ATTTCAGACT TCATTGAGAA ACTCCAGGTT
            121 GAGACAGGCC GGGATGTGGA TCGAGTCCAT GGTCAGAGCA GATGGGTTAT ATGGAAACAG
            181 AACTGGGAAC ACGTGCTTGG AGTCCAGCAG GAGCTGCTGA GGGTCATTCC TCTCTTGTAG
            241 CTGAGCCTGG ATTGTTCGTA TGAAGGACAC TGTGACCCGT TCCTCAAATT CATTCAAGGG
            301 AGTGTAGAGG TTTAAAATTT TGACAATCTG CTGGGTGCTG AGGGAGGTGC ACAGAGAGCA
            361 GATGGCCTCA GCATCCTCGT GGGTTTTCTT CTTCAGCTGG AGGAGCTGGG CTGCCTGGAT
            421 GAGGGGCTCC ATGGTCTGAA CTGCTCCACT TTGGTGAAGG TTTTTGCCCC GAAGCCACTC
            481 TTCCAGTTGA CTTATGTTGT ACCTGAGTTG CATGCCTGTG CTCCAGGAGC AGGCGTCTTT
            541 CCGCAGGAGT AGGTTGTTAA GAGTCACAGC GTTGATCATG TAGAAGAGCT GTTTGAACAC
            601 CTGCAGGATA ATCTCGGGGT CCAGACCCTG GTCACACAGG ACTGTATGGA AAGAATTCAT
            661 CTGGCGGACG ATGGCCTCCA GGCAATACGA ATTCTCTCCA TCCACCATGC TGGAGGAGCG
            721 CTTCCGGTAA CCAGTTGGTC TCACACCAGA CAGCCCCTGG ATACTCTCAT TTTCCAACAT
            781 GGCAGAAACT ATCATAGGCT GCAGCAAGCC CTCGGCGATT TTAATGAGCT GCTGATAGAT
            841 CTGAATGGAA AGGTCGCTTA GCACCTGGCG GTATTCAGTG AGGTCAAAGT TCTTGAGACA
            901 GTGCTCATTC TGCTTCGCAG TGTTCTGTGT CATGAAACCC TCATCACCAC TGTATTGCTT
            961 CAGACAGTGA AGGAAGCGGC AGGTGTTGGG ATAGCCCAGA ATGACGTCAT CTCAAGTCAT
           1021 CCATTGTGCT TCTTGAGGAC TTTCTATGCG ATGGATGGTT GGAAGCTCAG CAACGAATTG
          1081 CAATATGAGG TTCATCGATT GGGTGTAATT CG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Swiatecka-Urban, A., Myosin Vb is required for trafficking of the cystic fibrosis transmembrane conductance regulator in Rab11a-specific apical recycling endosomes in polarized human airway epithelial cells. J. Biol. Chem. 282 (32): 23725-23736 (2007). PMID 17462998.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl