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pcDNA3/hArf6(T27N)-HA

Expression vector of human ARF6, inactivating mutant T27N.

Catalog number RDB15351
Resource name pcDNA3/hArf6(T27N)-HA
Alternative name W43
Clone info. Expression vector of human ADP-ribosylation factor6 (ARF6), inactivating mutant T27N, tagged with HA epitope at C-ter.
Comment Expression was confirmed by the depositor with immunostaining. This clone contains a cDNA fragment corresponding to the 558nt to 1082nt of NM_001663.4 . Known differences : G to A substitution at 626nt (Ala, synonym) ; A to G substitution at 641nt (Thr, synonym).
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cels).
Gene/insert name human ARF6 cDNA
Depositor|Developer Nakayama, Kazuhisa |
Other clones in our bank
External Database
human ARF6
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。
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          RDB15351 pcDNA3/hArf6(T27N)-HA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3/hArf6(T27N)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15351).

          Reference section:

          Makyio, H., Ohgi, M., Takei, T., Takahashi, S., Takatsu, H., Katoh, Y., Hanai, A., Ueda, T., Kanaho, Y., Xie, Y., Shin, H.W., Kamikubo, H., Kataoka, M., Kawasaki, M., Kato, R., Wakatsuki, S., Nakayama, K., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15351_A7I5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert (hArf6 (T27N)), HA
          Sequence file: RDB15351_A7I5a.seq check
          >D03588B6_A7I5_1_CMV-Forward_D04_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTTGN NNNNNNGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTTGGTAC CGAGCTCGGA TCTGCCACCA TGGGGAAGGT
            181 GCTATCCAAA ATCTTCGGGA ACAAGGAAAT GCGGATCCTC ATGTTGGGCC TGGACGCAGC
            241 CGGCAAGAAC ACGATCCTGT ACAAGTTGAA GCTGGGCCAG TCGGTGACCA CCATTCCCAC
            301 TGTGGGTTTC AACGTGGAGA CGGTGACTTA CAAAAATGTC AAGTTCAACG TATGGGATGT
            361 GGGCGGCCAG GACAAGATCC GGCCGCTCTG GCGGCATTAC TACACTGGGA CCCAAGGTCT
            421 CATCTTCGTA GTGGACTGCG CCGACCGCGA CCGCATCGAT GAGGCTCGCC AGGAGCTGCA
            481 CCGCATTATC AATGACCGGG AGATGAGGGA CGCCATAATC CTCATCTTCG CCAACAAGCA
            541 GGACCTGCCC GATGCCATGA AACCCCACGA GATCCAGGAG AAACTGGGCC TGACCCGGAT
            601 TCGGGACAGG AACTGGTATG TGCAGCCCTC CTGTGCCACC TCAGGGGACG GACTCTATGA
            661 GGGGCTCACA TGGTTAACCT CTAACTACAA ATCTCTCGAG TATCCATACG ATGTACCAGA
            721 CTACGCAGAG ATCCACTAGT TCTAGANGGC CCTATTCTAT AGTGTCACCT AAATGCTAGA
            781 GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC
            841 CCCGTGCCTT NCTNGANCCT GGAANGTGCC NNNCCCNCNG TCCTTTCCTA ANAAAATGAN
            901 GAAATTGNAT CNCATTGTCT GANTANGNGN CNTTCTANTC TNGGGGGGNN GGGNNGGGGG
          961 NNNNANNNNN NNGGGGNNNN NNNNGGNANN NNNNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB15351_A7I5b.seq check
          >D03588B6_A7I5_1_SV40pro_F_F04_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNAN NNNTGNNGNN GCTTTTTTGG AGGCCTAGGC TTTTGCNAAA
             61 AGCTCCCGGG NGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
            121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
            661 CCGACGGCGA GGNTCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC
            781 ANGACATAGC GTTGGCTACN CGTGATATTG CTGAAGANCT TGGCGGCGAA TGNNCTGACN
            841 GCTTCCTCGN GCTTTACGGT ATCGCCGCNC CCGATTCGCA NCGCATCGCC TTCNATCGCC
            901 NTCTTGACNA NTNCNTCTGA NCGGGNNTCN GGGGGNTCNN AANGNNNGNN NNANNNACNN
            961 CNNANCNTGN CNNNCNCNNN NNTNNNANNN NNNCNNNCNN NCNNTNNNNN NNANNNNNNG
          1021 NNNTNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl