Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-HA-mouse MICAL-L1

Expression vector of mouse (MICAL-L1, N-terminal HA-tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kobayashi, H. and Fukuda, M., J. Cell Sci. 126 (11): 2424-2435, 2013)。 付加的提供条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15385_A7Ibp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,HA,insert 5'(MICAL-L1) Sequence file: RDB15385_A7Iba.seq
>D04916B3_A7Ib_2_EF1a-C_F_G05_20_ABI24.ab1     1 TAAGCATGAT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA    61 GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTCTCTA GAGCGGCCGC GGGAATTCGA   121 TTCCACCATG GGTTACCCAT ACGACGTCCC AGACTACGCT GGATCTATGG CGGGGCCGCG   181 GGGCGCTCTC CTGGCCTGGT GTCGCCGCCA GTGCGAGGGC TACCGCGGCG TGGACATCCG   241 CGACCTGAGC AGCTCCTTCC GGGACGGCCT GGCTTTCTGC GCCATCCTGC ACCGGCACCG   301 GCCCGACCTG CTCGACTTCC AATCTCTCTC CAAGGAAAAT GTCTTCGAGA ATAATCGATT   361 GGCCTTTGAA GTGGCTGAAA AGGAACTGGG CATCCCTGCT CTCCTGGACC CCAATGACAT   421 GGTCTCCATG AGTGTCCCTG ACTGTCTCAG CATCATGACC TATGTGTCCC AGTACTACAA   481 CCACTTCACC AGTTCTGGCC AAGCTGCTGC CTCACCACCC AAACCAGGAA AGGACCCAGC   541 ACCCCCCTCC CCCACATCCA CATCACCCGC TGTGCAGCCA GGAGAGGAGG CTCAGGGTGA   601 CGACCTCTCC CCAGACAGCC TGTCCGAGCA GGGCAAACAG CAGCCCCCGA GCAGCGCATG   661 CGCAGCCTGT GGGCAGCGGG TGCACCTGGT GCAGCGGTAC TTGGCGGAGG GCAGACTCTA   721 CCACCGGCAC TGCTTCCGAT GTCGGCAGTG TTCCAGCACG CTGGTCCCAG GCTCTTACAG   781 TAGTGGGCCC GAAGAAGGCA CCTTTGTGTG TGCAGAACGC TGCACCAGGC TGGGTCCGGG   841 AAGTCGGTCA GGAACTAGGC TCCTTTCACA GCAAAGGCAG CAGCCAGCGG CGGCAGAAGC   901 TAAAGATGCA GAGGATAATG ACCCAAGCCC TGAGTGTGGC TGCAGTGGCT GAGGCAGACA   961 GGCTCCAGGG CCAGCCTCGA GTACAGTTTC CACACCCCCA ATGAGCACTC TTCCCAGCCA 1021 GCCCAGCACT GACCAGTCCA CTTGTGACTG ACCAACCTGG CCCCCAGGGA CGGCATC //
Primer: hGCSF_R (Pr0031) Region: insert 3' Sequence file: RDB15385_A7Ibb.seq
>D04916B3_A7Ib_2_hGCSF_R_C01_03_ABI08.ab1     1 TTTGGAAACG TTAAAACTCC TCAGGGTCCC AGGGTGGGGA CCCTCACTCT AGAGGCGGCC    61 GCTATGGTCG ACTCAGCTCT TGTCCCCTGG GGCCTTGCTC TTGGCTTCTC GTTTATTCCC   121 CAGAAACTTC AGCACCTTTA TGGTCTTGAA CTTGCCTTTC TTCTTACTGT CAGACTCGGC   181 CTCTCTTTGG AAATCTTTCT TCTTGATCAT AGTTTCCAAC ATCTTGTCTT CCTCCTCTTC   241 CCTCTGCCGG TCCTCGTCCA GGCAGTTCAC GATGGCATCT CGCTGCTCGA TGAGGGTCAT   301 GAGTTCCTGC ATCAGCACCT TCTCTCTGGC CCGATCCTCA TCTGTCCAGT CCTTTTCTGG   361 CTTGTTGAGA AGGCACCGGA GCTCGAACTC CACATCAGCC TGGCGCTGCT CCAGGTTCTG   421 CTGCTTGAAG ACATAGATGA GCTCCGACTC TCGCCGCACT AGTAGGTGCT TCTCATGGAT   481 GAGTTTGAAC CAGTCCACCA GCATGTCGTC CTCGCTGCCC TCATTTGCTC CGCCACGCAG   541 CTTCTCCTCC AACAGAACCC CACTGTGTTC CAGGGCATCC AGCTGGCGCT CAATATTGTC   601 CATCTCACCG TAGATGTCCT CCTCGGGAAT GTACTGGTCA GCCTGGACCT TGCGTTTGAT   661 GAGTGGAAAG CCGTGTCCGG GGGCAGGTGG CCTCACAGGC TTGGCTCCTT TTGGTGGCCT   721 TCCTTACAGT TGGAGACGCG GAGGGAGATG GTTTCCGGTT GAAAGGATTC TCCTTGCAGG   781 AAGACTTGAT TTGCAGCTGT GGGGATGCGC TCCCCCACCG GGAGGGGCAG GGTTCCGGTC   841 TCCAGCCCAA CAAAGAAAGG GGTGGGGGGT GGCACCCACC TGAAAGGGCC TTGGTAGAAA   901 TGGGGTAACC CCCGCGCTGC CCCCTTCGTC CTGGACCCTG GCGGGCATTT TGTTCAACCA   961 CCTAAGGCTT GCCCCCTAGC CCTCCCCCAA GAAAGACGAC CAGGGTAAAG GAGTTGGGCT  1021 TGAAATGGGG GGTGAACAGC AGGTTATCCC AAAAGGTGGC CCAAGGGGGT AACCCAGGGC  1081 CCACCGCTGC CACCAGACCA AGGAATCCCG AAGGGAAAGC TTCCCTTGGG GCTTACCGGC 1141 CACGGCCGGG CCCTCCCAAA AAGAGGCCTT CTTGTCCAAC TTGGTAGGGC CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl