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pMRX-puro-HA-mouse PAT4 (#RDB15392)

Retroviral expression vector of mouse PAT, N-terminal HA-tag.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Matsui, T., EMBO Rep. 14 (5): 450-457 (2013). PMID 23478338. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Retroviral expression vector of mouse proton assisted amino acid transporter 4 (PAT4) [Slc36a4 in NCBI], tagged with N-terminal HA, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pMRX-IRES-puro (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Slc36a4 cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Matsui, T. and Fukuda, M., EMBO Rep. 14 (5): 450-457, 2013)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Matsui, T. and Fukuda, M., EMBO Rep. 14 (5): 450-457, 2013)。
付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB15392 pMRX-puro-HA-mouse PAT4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMRX-puro-HA-mouse PAT4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15392).

Reference section:

Matsui, T., Fukuda, M., Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4. EMBO Rep. 14 (5): 450-457 (2013). PMID 23478338. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Matsui, T., Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4. EMBO Rep. 14 (5): 450-457 (2013). PMID 23478338. [PubMed] [Article] [RRC of NBRP]