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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28/Dronpa-theta-D4

Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.

Catalog number RDB15469
Resource name pET28/Dronpa-theta-D4
Clone info. Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.
Vector backbone Plasmid
Gene/insert name Clostridium perfringens perfringolysin O (pfoA)
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Clostridium perfringens perfringolysin O (pfoA)

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Please visit Information of Request for Distribution.[link] 
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC.
          2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Mizuno, H., Abe, M., Dedecker, P., Makino, A., Rocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A. (2011) Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553.
          4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
          5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件:
          本件リソースの使用は学術機関での学術研究に限る。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 提供条件は英文のまま記載してください。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15469 pET28/Dronpa-theta-D4 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pET28/Dronpa-theta-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15469).

          Reference section:

          Mizuno, H., Abe, M., Dedecker, P., ARocha, S., Ohno-Iwashita, Y., Hofkens, J., Kobayashi, T., Miyawaki, A., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Visualization of Organelles (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15469_A7Jqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBR_BamHI_R2 ()
          Sequence file: RDB15469_A7Jqa.seq check
          >D04498J6_A7Jq_1_1_PBR-BamHI-R2_A04_01_ABI24.ab1
              1 GTGCGTGGCA GCCATTCGAC GTGAGCGGCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT
             61 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC
            121 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC CATGGGCAGC AGCCATCATC
            181 ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCGG TACCATGAGT GTGATTAAAC
            241 CAGACATGAA GATCAAGCTG CGTATGGAAG GCGCTGTAAA TGGACACCCG TTCGCGATTG
            301 AAGGAGTTGG CCTTGGGAAG CCTTTCGAGG GAAAACAGAG TATGGACCTT AAAGTCAAAG
            361 AAGGCGGACC TCTGCCTTTC GCCTATGACA TCTTGACAAC TGTGTTCTGT TACGGCAACA
            421 GGGTATTCGC CAAATACCCA GAAAATATAG TAGACTATTT CAAGCAGTCG TTTCCTGAGG
            481 GCTACTCTTG GGAACGAAGC ATGAATTACG AAGACGGGGG CATTTGTAAC GCGACAAACG
            541 ACATAACCCT GGATGGTGAC TGTTATATCT ATGAAATTCG ATTTGATGGT GTGAACTTTC
            601 CTGCCAATGG TCCAGTTATG CAGAAGAGGA CTGTGAAATG GGAGCCATCC ACTGAGAAAT
            661 TGTATGTGCG TGATGGAGTG CTGAAGGGTG ATGTTAACAT GGCTCTGTCG CTTGAAGGAG
            721 GTGGCCATTA CCGATGTGAC TTCAAAACTA CTTATAAAGC TAAGAAGGTT GTCCAGTTGC
            781 CAGACTATCA CTTTGTGGAC CACCACATTG AGATTAAAAG CCACGACAAA GATTACAGTA
            841 ATGTTAATCT GCATGAGCAT GCCGAAGCGC ATTCTGAGCT GCCGAGGCAG GCCAAGAAGC
            901 TTAAGGGAAA AATAAACTTA GATCATAGTG GAGCCTATGT TGCACAGTTT GAAGTAGCCT
            961 GGGATGAAGT TTCATATGAC AAAGAAGGAA ATGAAGTTTT AACTCATAAA ACATGGGATG
           1021 GGAAATTATC AAGATAAAAC AGCTTCACTA TTCAACAGTA AATACCTCTT GAGGCTAATG
           1081 CAAGAAATAT AGATTAAAAG CCAGAGAGTG TACAGGGTCC TTGCTTGGGC AATTGGCTGG
          1141 GAAGAAGAAT GGTTACTA
          //
          Primer: T7 terminator ()
          Sequence file: RDB15469_A7Jqb.seq check
          >D04498J6_A7Jq_1_1_T7 terminator_C04_07_ABI24.ab1
              1 AAATTGGCTT TCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGC
             61 TCGAGTTAAT TGTAAGTAAT ACTAGATCCA GGGTATAAAG TTGTTCCCCA TATTGAAACA
            121 TTTATATTAT TTGTTAATGG AACATCATAT TCACTTATAA CATCTCTCCA CCATTCCCAA
            181 GCAAGGCCTG TACACTCTCT TGCTTTTATT CTTATATTTC TTGCATTAGC TTCAAGAGGT
            241 ATTACTGTTG AATAGTGAGC TGTTTTATCT TGATAATTTC CATCCCATGT TTTATGAGTT
            301 AAAACTTCAT TTCCTTCTTT GTCATATGAA ACTTCATCCC AGGCTACTTC AAACTGTGCA
            361 ACATAGGCTC CACTATGATC TAAGTTTATT TTTCCCTTAA GCTTCTTGGC CTGCCTCGGC
            421 AGCTCAGAAT GCGCTTCGGC ATGCTCATGC AGATTAACAT TACTGTAATC TTTGTCGTGG
            481 CTTTTAATCT CAATGTGGTG GTCCACAAAG TGATAGTCTG GCAACTGGAC AACCTTCTTA
            541 GCTTTATAAG TAGTTTTGAA GTCACATCGG TAATGGCCAC CTCCTTCAAG CGACAGAGCC
            601 ATGTTAACAT CACCCTTCAG CACTCCATCA CGCACATACA ATTTCTCAGT GGATGGCTCC
            661 CATTTCACAG TCCTCTTCTG CATAACTGGA CCATTGGCAG GAAAGTTCAC ACCATCAAAT
            721 CGAATTTCAT AGATATAACA GTCACCATCC AGGGTTATGT CGTTTGTCGC GTTACAAATG
            781 CCCCCGTCTT CGTAATTCAT GCTTCGTTCC CAAGAGTAGC CCTCAGGAAA CGACTGCTTG
            841 AAATAGTCTA CTATATTTTC TGGGTATTTG GCGAATACCC TGTTGCCGTA ACAGAACACA
            901 GTTGTCAAGA TGTCATAGGC GAAAGGCAGA GGTCCGCCTT CTTTGACTTT AAGGTCCATA
            961 CTCTGTTTTC CCTCGAAAGG CTTCCCAAGG CCAACTCCTT CAATCGCGAA CGGGTGTCCA
           1021 TTTACAGCGC CTTCCATACG CAGCTTGATC TTCATGTCTG GGTTTTAATC ACACTCATGT
           1081 ACGCTGGCCG CGCGGCACCA GGCCGCTGCT GTGAATGATG AATGATGGAT TGACTGCCTG
          1141 GCCAATGGGT ATAATCCTTC CCATTCCTA
          //
          Primer: lacIpro_F ()
          Sequence file: RDB15469_A7Jqc.seq check
          >D04498J6-1_A7Jq_lacpro_F_C09_09_ABI24.ab1
              1 GGTAGCATGA TGCGCGGAGA GAGTCATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT
             61 ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG
            121 CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT
            181 ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG
            241 CCACATCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG
            301 CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT
            361 GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC
            421 CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT
            481 TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGACGGTA
            541 CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG
            601 GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC
            661 GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC
            721 AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG
            781 ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG
            841 ATATCTCGGT AGTGGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA
            901 ACCACCATCA AACAGGATTT TTCGCCTGCT GGGGCAAAAC CAGCGTGGAC CGCTTGCTGC
            961 AACTCTCTCA GGGCCAGGCC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGCTGGAA
           1021 AGAAACCACC CTGGCGCCCA ATACGCCAAC CGCCTCTCCC GCGCGGTTGT GATCCATAAT
          1081 TGCAGCCTGG CCACGAACAA GGTTTTCGCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Mizuno, H., Fluorescent probes for superresolution imaging of lipid domains on the plasma membrane. Chem. Sci. 2: 1548-1553 (2011). [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl