Please review the QC test results indicated by icon below as well as clone information before placing your order.

pENTER-EGFR Del19

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-activating mutation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Uchibori, K et al. Nat. Commun. 8: 14768, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15489_A7K8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2 (Pr0531) Region: attL1, insert 5' Sequence file: RDB15489_A7K8d.seq
>D04925A2_A7K8_2_rrnBT1ter_F2_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCCTTTCG TTTNNTTTGA NGCCTGGCAG TTCCCTACTC    61 TCGCGTTAAC GCTAGCATGG ATGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTC   121 TTAAGCTCGG GCCCCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC   181 AAATTGATGA GCAATGCTTT TTTATAATGC CAACTTTGTA CAAAAAAGCA GGCTCCGCGG   241 CCGCCCCCTT CACCATGCGA CCCTCCGGGA CGGCCGGGGC AGCGCTCCTG GCGCTGCTGG   301 CTGCGCTCTG CCCGGCGAGT CGGGCTCTGG AGGAAAAGAA AGTTTGCCAA GGCACGAGTA   361 ACAAGCTCAC GCAGTTGGGC ACTTTTGAAG ATCATTTTCT CAGCCTCCAG AGGATGTTCA   421 ATAACTGTGA GGTGGTCCTT GGGAATTTGG AAATTACCTA TGTGCAGAGG AATTATGATC   481 TTTCCTTCTT AAAGACCATC CAGGAGGTGG CTGGTTATGT CCTCATTGCC CTCAACACAG   541 TGGAGCGAAT TCCTTTGGAA AACCTGCAGA TCATCAGAGG AAATATGTAC TACGAAAATT   601 CCTATGCCTT AGCAGTCTTA TCTAACTATG ATGCAAATAA AACCGGACTG AAGGAGCTGC   661 CCATGAGAAA TTTACAGGAA ATCCTGCATG GCGCCGTGCG GTTCAGCAAC AACCCTGCCC   721 TGTGCAATGT GGAGAGCATC CAGTGGCGGG ACATAGTCAG CAGTGACTTT CTCAGCAACA   781 TGTCGATGGA CTTCCNGANN CACCTNNGCA GCTGCCAAAA GTGTGATCCA AGCTGTCCCA   841 ATGGGANCTG CTGGGGTGCA NGANANGANA NNTGCCAGAA ACTGANNAAA ATCATCTGNG   901 CCCANCAGNG CTCCNGGNNC TGCCGNGGCA NNNCCCCCNA NNNNNNGCTN NCNNNACCAG   961 NGNNCTGCNN NNNGCNNNNN NCCCNNNGGN NANCNNNNGN CNNNNNNNNN CNNNNAATNN 1021 CNNNNNNNAN NNNNNNNNN //
Primer: pDONR_R (Pr0071) Region: attL2, insert 3' Sequence file: RDB15489_A7K8e.seq
>D04925A2_A7K8_2_pDONR_R_B08_05_ABI24.ab1     1 NNNNNNNNNN NGNNNNNCNN TGNNNNTGTA NTACGACTCN CTATAGGGGA NATCAGCTGG    61 ATGGCAAATA ATGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATAA   121 GCAATGCTTT CTTATAATGC CAACTTTGTA CAAGAAAGCT GGGTCGGCGC GCCCACCCTT   181 TCATGCTCCA ATAAATTCAC TGCTTTGTGG CGCGACCCTT AGGTATTCTG CATTTTCAGC   241 TGTGGAGCCC TTAAAGATGC CATTTGGCTT GGCTTCCTTG GGAAAGAAGT CCTGCTGGTA   301 GTCAGGGTTG TCCAGGCTAA TTTGGTGGCT GCCTTTCTGG GCCCAGTGGG CAGGGCTGTC   361 GAATGTGCTG TTGACACAGG TGGGCTGGAC AGTGTTGAGA TACTCGGGGT TGCCCACTGC   421 AGTGCTGTGG GGGTCCTGGT AGTGTGGGTC TCTGCTGGGC GCGGGGTTCA GAGGCTGATT   481 GTGATAGACA GGATTCTGCA CAGAGCCAGC GGGCCTTTTG GGAACGGACT GGTTTATGTA   541 TTCAGGCACT GGGAGGAAGG TGTCGTCTAT GCTGTCCTCA GTCAAGGCGC CTGTGGGGTC   601 TGAGCTGTAT CGCTGCAAGA AGCTGTCTTC CTTGATGGGA CAGCTTTGCA GCCCATTTCT   661 ATCAATGCAA GCCACGGTGG AATTGTTGCT GGTTGCACTC AGAGAGCTCA NGANGGGAGT   721 CCGTGACGTG GANGGGCTGC TGAAGAAGCC CTGCTGTGGG ANGANGTACT CGTCGGCATC   781 CACCACGTCG TCCATGTCTT CTTCATCCAT CANGGCACGG TANAAGTTGG ANTCTGTANG   841 ANTNNNCAAA TGCATTCTTT CNTCCCCCNN AANGACAAGG NANCGCNGGG GGNNCTCGGN   901 NCATTTNNNA NAANNCNATG NTCNACTCNN NGNANNTTTG GNNNANTATC NNCNNNNNNN 961 NNN //
Primer: EGFR-F5 (Pr0313) Region: insert mid (del19) Sequence file: RDB15489_A7K8f.seq
>D04925A2_A7K8_2_EGFR-F5_B09_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGNNGGTG GGGGCCCTCC TCTTGCTGCT GGTGGTGGCC    61 CTGGGGNTCG GCCTCTTCAT GCGAAGGCGC CACATCGTTC GGAAGCGCAC GCTGCGGAGG   121 CTGCTGCAGG AGAGGGAGCT TGTGGAGCCT CTTACACCCA GTGGAGAAGC TCCCAACCAA   181 GCTCTCTTGA GGATCTTGAA GGAAACTGAA TTCAAAAAGA TCAAAGTGCT GGGCTCCGGT   241 GCGTTCGGCA CGGTGTATAA GGGACTCTGG ATCCCAGAAG GTGAGAAAGT TAAAATTCCC   301 GTCGCTATCA AGACATCTCC GAAAGCCAAC AAGGAAATCC TCGATGAAGC CTACGTGATG   361 GCCAGCGTGG ACAACCCCCA CGTGTGCCGC CTGCTGGGCA TCTGCCTCAC CTCCACCGTG   421 CAACTCATCA CGCAGCTCAT GCCCTTCGGC TGCCTCCTGG ACTATGTCCG GGAACACAAA   481 GACAATATTG GCTCCCAGTA CCTGCTCAAC TGGTGTGTGC AGATCGCAAA GGGCATGAAC   541 TACTTGGAGG ACCGTCGCTT GGTGCACCGC GACCTGGCAG CCAGGAACGT ACTGGTGAAA   601 ACACCGCAGC ATGTCAAGAT CACAGATTTT GGGCTGGCCA AACTGCTGGG TGCGGAAGAG   661 AAAGAATACC ATGCAGAAGG AGGCAAAGTG CCTATCAAGT GGATGGCATT GGAATCAATT   721 TTACACAGAA TCTATACCCA CCAGAGTGAT GTCTGGAGCT ACGGGGTGAN CGTTTGGGAG   781 TTGANGACCT TTGGATCCNA GCCATATGAC GGANTCCCTG CCAGCGAGAT CTCCTCCATC   841 CTGGANAAAG GANAANGCCT CCCTCANCCA CCCATATGTA CCATCNATGT CTACATGATC   901 ATGGNCAAGN GCNNGNNGAN AGANNNANAN AGTCGNCCNA ANTNNCNNNA GTNNNTNATC 961 GNANTTCNNC NAAANNNNCN NNNNNCNNCN NNNNNNNNCN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl