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pENTER-EGFR T790M/del19

Gateway(R) entry vector of human epidermal growth factor receptor (EGFR), EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.

Catalog number RDB15490
Resource name pENTER-EGFR T790M/del19
Clone info. Gateway(R) entry vector of human epidermal growth factor receptor (EGFR) mutant, T790M and deletion in exon 19, EGFR-tyrosine kinase inhibitor (TKI) resistant and activating mutations.
Comment cDNA was obtained from HCC-827 cells. Expression was confirmed by the depositor with western blotting after cloned into pLenti6.3 vector (by LR clonase II reaction). This clone contains a cDNA fragment corresponding to the 258nt to 3890nt of NM_005228.4. Known differences (at least) : 15nt (del19) deletion at 2493 to 2507nt ; C to T substitution at 2626nt (T790M).
Vector backbone pENTR (plasmid)
Size of vector backbone 2.6 kb
Selectable markers Kan^r
Gene/insert name human EGFR cDNA
Depositor|Developer Katayama, Ryohei |
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External Database
human EGFR

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Uchibori, K et al. Nat. Commun. 8: 14768, 2017)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Uchibori, K et al. Nat. Commun. 8: 14768, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

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          RDB15490 pENTER-EGFR T790M/del19 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pENTER-EGFR T790M/del19 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15490).

          Reference section:

          Uchibori, K., Inase, N., Araki, M., Kamada, M., Sato, S., Okuno, Y., Fujita, N., Katayama, R., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15490_A7K8p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: rrnBT1ter_F2 (Pr0531)
          Region: rrnB T1 ter,attL1,insert 5'
          Sequence file: RDB15490_A7K8d.seq check
          >D04925A3_A7K8_1_rrnBT1ter_F2_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN CCTTTCGTTT NNTTTGATGC CTGGCAGTTC CCTACTCTCG
             61 CGTTAACGCT AGCATGGATG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTCTTA
            121 AGCTCGGGCC CCAAATAATG ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA
            181 TTGATGAGCA ATGCTTTTTT ATAATGCCAA CTTTGTACAA AAAAGCAGGC TCCGCGGCCG
            241 CCCCCTTCAC CATGCGACCC TCCGGGACGG CCGGGGCAGC GCTCCTGGCG CTGCTGGCTG
            301 CGCTCTGCCC GGCGAGTCGG GCTCTGGAGG AAAAGAAAGT TTGCCAAGGC ACGAGTAACA
            361 AGCTCACGCA GTTGGGCACT TTTGAAGATC ATTTTCTCAG CCTCCAGAGG ATGTTCAATA
            421 ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT TATGATCTTT
            481 CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC AACACAGTGG
            541 AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC GAAAATTCCT
            601 ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG GAGCTGCCCA
            661 TGAGAAANTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC CCTGCCCTGT
            721 GCAATGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC AGCAACATGT
            781 CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGNTCCAAGC TGTCCCAATN
            841 NNANCTGCTG GGGTGCANGA NANGANAANT GCCAGAAACT GANCAAAATC ATCTGNGCCC
            901 AGCAGNGCNC CGGGCGCTGC CNNGGCAAGN CCCCCNANNN NNNGCTGCCA CAACCANNNN
          961 NNTGNNNNNN NNCNNNNCCC CNGGNNNNNC NNNNNNNNNN
          //
          Primer: pDONR_R (Pr0071)
          Region: attL2,insert 3'
          Sequence file: RDB15490_A7K8e.seq check
          >D04925A3_A7K8_1_pDONR_R_C08_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNCN NTGNNNNTGT AATACGACTC ACTATAGGGG ANATCAGCTG
             61 GATGGCAAAT AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATA
            121 AGCAATGCTT TCTTATAATG CCAACTTTGT ACAAGAAAGC TGGGTCGGCG CGCCCACCCT
            181 TTCATGCTCC AATAAATTCA CTGCTTTGTG GCGCGACCCT TAGGTATTCT GCATTTTCAG
            241 CTGTGGAGCC CTTAAAGATG CCATTTGGCT TGGCTTCCTT GGGAAAGAAG TCCTGCTGGT
            301 AGTCAGGGTT GTCCAGGCTA ATTTGGTGGC TGCCTTTCTG GGCCCAGTGG GCAGGGCTGT
            361 CGAATGTGCT GTTGACACAG GTGGGCTGGA CAGTGTTGAG ATACTCGGGG TTGCCCACTG
            421 CAGTGCTGTG GGGGTCCTGG TAGTGTGGGT CTCTGCTGGG CGCGGGGTTC AGAGGCTGAT
            481 TGTGATAGAC AGGATTCTGC ACAGAGCCAG CGGGCCTTTT GGGAACGGAC TGGTTTATGT
            541 ATTCAGGCAC TGGGAGGAAG GTGTCGTCTA TGCTGTCCTC AGTCAAGGCG CCTGTGGGGT
            601 CTGANCTGTA TCGCTGCAAG AAGCTGTCTT CCTTGATGGG ACAGCTTTGC AGCCCATTTC
            661 TATCNATGCA AGCCACGGNG GAATTGTTGC TGGTTGCACT CAGANANCTC NNNANGGGAN
            721 TCCGTGANGN GGANGGGCTG CTGNANAANC CCTGCTGTGG GNNGANGNAN TCGNCNGCNT
            781 CCNCCNCNNC NTNCNTGNCN NCNNNNTNCN TCNNGNNNNN GNANANNNNN GNANNCNGNN
          841 NNANTNNNNN AANGCNTNNN TNNNNNCNNN NNNNNNN
          //
          Primer: EGFR-F5 (Pr0313)
          Region: insert mid(T790M/del19)
          Sequence file: RDB15490_A7K8f.seq check
          >D04925A3_A7K8_1_EGFR-F5_C09_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNNG GTGGGGGCCC TCCTCTTGCT GCTGGTGGTG
             61 GCCCTGGGGN NCGGCCTCTT CNTGCGAAGG CGCCACATCG TTCGGAAGCG CACGCTGCGG
            121 AGGCTGCTGC AGGAGAGGGA GCTTGTGGAG CCTCTTACAC CCAGTGGAGA AGCTCCCAAC
            181 CAAGCTCTCT TGAGGATCTT GAAGGAAACT GAATTCAAAA AGATCAAAGT GCTGGGCTCC
            241 GGTGCGTTCG GCACGGTGTA TAAGGGACTC TGGATCCCAG AAGGTGAGAA AGTTAAAATT
            301 CCCGTCGCTA TCAAGACATC TCCGAAAGCC AACAAGGAAA TCCTCGATGA AGCCTACGTG
            361 ATGGCCAGCG TGGACAACCC CCACGTGTGC CGCCTGCTGG GCATCTGCCT CACCTCCACC
            421 GTGCAGCTCA TCATGCAGCT CATGCCCTTC GGCTGCCTCC TGGACTATGT CCGGGAACAC
            481 AAAGACAATA TTGGCTCCCA GTACCTGCTC AACTGGTGTG TGCAGATCGC AAAGGGCATG
            541 AACTACTTGG AGGACCGTCG CTTGGTGCAC CGCGACCTGG CAGCCAGGAA CGTACTGGTG
            601 AAAACACCGC AGCATGTCAA GATCACAGAT TTTGGGCTGG CCAAACTGCT GGGTGCGGAA
            661 GAGAAAGAAT ACCATGCAGA AGGAGGCAAA GTGCCTATCA AGTGGATGGC ATTGGAATCA
            721 ATTTTACACA GAATCTATAC CCACCAGAGT GATGTCTGGA GCTACGGNNT GANCGTTTGG
            781 GAGTTGATGA NCTTTGGATC CAAGCCATAT GACGGAATCC CTGCCNGCGA GATCTCCTCC
            841 NTCCTNGANA AAGGANAACG CCNCCNTCAG CCNCCCATAT GTANCNTCGA NGNCTACATG
            901 ATCATGGNCN ANNGCTGGNT GATAGNNGCN NANAGNCGCC NNAANTTNCN NNNNNNGATC
          961 ATCNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Uchibori, K., Brigatinib combined with anti-EGFR antibody overcomes osimertinib resistance in EGFR-mutated non-small-cell lung cancer. Nat. Commun. 8: 14768 (2017). PMID 28287083.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl