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pmCherry2-hGRalpha1 (#RDB15749)

Expression vector of human GR alpha1

Clone info. Expression vector of human glucocorticoid receptor (GR alpha1), fused with tandem mCherry at N-terminal, CMV promoter, see Fig. S1 of Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017.
Comment Expression was confirmed by the depositor with fluorescence cross-correlation spectroscopy (FCCS) and microscopic observation.
Vector backbone pmCherry2-C1 (plasmid)
Gene/insert name human NR3C1 cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017).
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必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15749 pmCherry2-hGRalpha1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmCherry2-hGRalpha1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15749).

Reference section:

Tiwari, M., Oasa, S., Yamamoto, J., Mikuni, S., Kinjo, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15749_B3Aip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40polyA-R (Pr0109)
Region: insert 3'
Sequence file: RDB15749_B3Aia.seq check
>D05001C9_B3Ai_2_SV40polyA-R_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TCTANNAATG TGGTATGGCT GATTATGATC
   61 AGTTATCTAG ATCNGGTGGA TCCCGTGCTT TTGATGAAAC AGAAGTTTTT TGATATTTCC
  121 ATTTGAATAT TTTGGTATCT GATTGGTGAT GATTTCAGCT AACATCTCGG GGAATTCAAT
  181 ACTCATGGTC TTATCCAAAA ATGTTTGGAA GCAATAGTTA AGGAGATTTT CAACCACTTC
  241 ATGCATAGAA TCCAAGAGTT TTGTCAGTTG ATAAAACCGC TGCCAGTTCT GGCTGGAGTT
  301 TCCTTCCCTC TTGACAATGG CTTTTCCTAG CTCTTTGATG TAGGTCATTC TAATTTCATC
  361 AAATAGCTCT TGGCTCTTCA GACCGTCCTT AGGAACTGAA GAGAGAAGCA GTAAGGTTTT
  421 CATACAGAGA TACTCTTCAT AAGATACCTG AAGCCTGTGT AACTCAGAGG AAACATACAG
  481 CATGTGTTTA CATTGGTCGT ACATGCAGGG TAGAGTCATT CTCTGCTCAT TAATAATCAG
  541 ATCAGGAGCA AAACACAGCA GGTTTGCACT TGATTGTCTA TATGATCTCC ACCCCAGAGC
  601 AAATGCCATA AGAAACATCC AGGAGTACTG CAGTAGGGTC ATTTGGTCAT CCAGGTGTAA
  661 GTTCCTGAAA CCTGGTATTG CCTTTGCCCA TTTCACTGCT GCAATCACTT GCCGCCCTCC
  721 TAACATGTTG AGCGTAGTCA TGATCCTCCA AGTTGAGTCT GGAACAGAGC TATCATATCC
  781 TGCATATAAC ACTTCAGGTT CAATAACCTC CAACAGNGAC ACCAGGGTAN GGGTGAGTTG
  841 TGGTAACGTT GCANGAACTA TTGTTTTGNN NCCNGGATTT TCANANGNTT CNTGTGAGNN
  901 TCCTGTAGNG NNCTGCTGAN NNNCTTTTAT TTTTTTTCTT NGNTTTTCNA GNTNNNNNGN
  961 NCATTCNNGC CNGAANANNT TTTNCGANAN NNGNCNNGNT GGGNNNNTTT TNNNNNNANT
1021 TTNNNNNNNN ANGNNNNNNN TNNNNNNNNN N
//
Primer: hGRalpha-N_R (Pr0534)
Region: insert 5',mCherry.2 3'
Sequence file: RDB15749_B3Aib.seq check
>D05001C9_B3Ai_2_hGRalpha-N_R_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNTNNGNNN CTGNNTGAGA AGCGACAGCC NGTGAGGGTG AAGACGCAGA
   61 AACCTTCACA GTAGCTCCTC CTCTTAGGGT TTTATAGAAG TCCATCACAT CTCCCCTCTC
  121 CTGAGCAAGC ACACTGCTGG GGTTTTCTTC TCTACCAGGA GTTAATGATT CTTTGGAGTC
  181 CATCAGTGGA GCTCGAGATC TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGCCGGTGGA
  241 GTGGCGGCCC TCGGCGCGCT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT
  301 GATGTCCAGC TTGATGTTCA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT
  361 GTAGGTGGTC TTGACCTCGG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT
  421 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC
  481 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC
  541 CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC
  601 GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA
  661 GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA
  721 CAGGATGTCC CAGGCGAAGG GCAGGGGGCC GCCCTTGGTC ACCTTCAGCT TGGCGGTCTG
  781 GGTGCCCTCG TANGGGCGGN CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC
  841 GGANCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGANGGNNN TGTTGTCCTC
  901 CTCGCCCTNG GTANCGCGGC CNTCNGCGCG CTCGNACTGT TCCNCGATGG NGNANNCCNC
  961 NNNGTGGGAN GNGNTGNNNA GNNNGNTGNT CACNNNGNNN NNNNNGNNNN CTGNNNNNNN
1021 NNN
//
Primer: hGRalpha-M_F (Pr0533)
Region: insert mid
Sequence file: RDB15749_B3Aic.seq check
>D05001C9_B3Ai_2_hGRalpha-M_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNAAGGNN AATGGAGNNC TGGTTTTGTC AAGCCCCAGT
   61 AATGTAACAC TGCCCCAAGT GAAAACAGAA AAAGAAGATT TCATCGAACT CTGCACCCCT
  121 GGGGTAATTA AGCAAGAGAA ACTGGGCACA GTTTACTGTC AGGCAAGCTT TCCTGGAGCA
  181 AATATAATTG GTAATAAAAT GTCTGCCATT TCTGTTCATG GTGTGAGTAC CTCTGGAGGA
  241 CAGATGTACC ACTATGACAT GAATACAGCA TCCCTTTCTC AACAGCAGGA TCAGAAGCCT
  301 ATTTTTAATG TCATTCCACC AATTCCCGTT GGTTCCGAAA ATTGGAATAG GTGCCAAGGA
  361 TCTGGAGATG ACAACTTGAC TTCTCTGGGG ACTCTGAACT TCCCTGGTCG AACAGTTTTT
  421 TCTAATGGCT ATTCAAGCCC CAGCATGAGA CCAGATGTAA GCTCTCCTCC ATCCAGCTCC
  481 TCAACAGCAA CAACAGGACC ACCTCCCAAA CTCTGCCTGG TGTGCTCTGA TGAAGCTTCA
  541 GGATGTCATT ATGGAGTCTT AACTTGTGGA AGCTGTAAAG TTTTCTTCAA AAGAGCAGTG
  601 GAAGGACAGC ACAATTACCT ATGTGCTGGA AGGAATGATT GCATCATCGA TAAAATTCGA
  661 AGAAAAAACT GCCCAGCATG CCGCTATCGA AAATGTCTTC AGGCTGGAAT GAACCTGGAA
  721 GCTCGAAAAA CAAAGAAAAA AATAAAAGGA ATTCAGCANG NNACTACAGG AGTCTCACAA
  781 GAAACCTCTG ANNATCCTGN TAACAAAACA ATAGTTCCTG CAACGTTACC ACAACTCACC
  841 CCTACCCTGG TGTCACTGTT GGANGNTATT GAANNTGAAG TGNTATATGC ANGANANGAT
  901 AGCTCTGNTC CNGANTCNAC TNGGANGANC ATGANTACNC TCNANATGNN ANGANGGNGG
  961 NNAGNGATTG CAGCANTGAA ATGGNNAAAN GNNNTACCNN NTTTCNNNNN TTANNNNNNG
1021 NNNGANNNAN NGNNCNNNNN NNNNN
//
Primer: RDB15749_F1 (Pr1284)
Region: insert mid
Sequence file: RDB15749_B3Aid.seq check
>D05001C9_B3Ai_2_RDB15749_F1_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNGTG GNAGGNNAGC ACAATTACCT ATGTGCTGGA
   61 AGGAATGATT GCATCATCGA TAAAATTCGA AGAAAAAACT GCCCAGCATG CCGCTATCGA
  121 AAATGTCTTC AGGCTGGAAT GAACCTGGAA GCTCGAAAAA CAAAGAAAAA AATAAAAGGA
  181 ATTCAGCAGG CCACTACAGG AGTCTCACAA GAAACCTCTG AAAATCCTGG TAACAAAACA
  241 ATAGTTCCTG CAACGTTACC ACAACTCACC CCTACCCTGG TGTCACTGTT GGAGGTTATT
  301 GAACCTGAAG TGTTATATGC AGGATATGAT AGCTCTGTTC CAGACTCAAC TTGGAGGATC
  361 ATGACTACGC TCAACATGTT AGGAGGGCGG CAAGTGATTG CAGCAGTGAA ATGGGCAAAG
  421 GCAATACCAG GTTTCAGGAA CTTACACCTG GATGACCAAA TGACCCTACT GCAGTACTCC
  481 TGGATGTTTC TTATGGCATT TGCTCTGGGG TGGAGATCAT ATAGACAATC AAGTGCAAAC
  541 CTGCTGTGTT TTGCTCCTGA TCTGATTATT AATGAGCAGA GAATGACTCT ACCCTGCATG
  601 TACGACCAAT GTAAACACAT GCTGTATGTT TCCTCTGAGT TACACAGGCT TCAGGTATCT
  661 TATGAAGAGT ATCTCTGTAT GAAAACCTTA CTGCTTCTCT CTTCAGTTCC TAAGGACGGT
  721 CTGAAGAGCC AAGAGCTATT TGATGAAATT AGAATGACCT ACATCAAAGA GCTAGGAAAA
  781 GCCATTGTCA AGAGGGAANG AAACTCCAGC CAGAACTGGC AGCGGTTTTA TCAACTGACA
  841 AAACTCTTGN ATTCTATGCA TGAAGTGGTT GAAAATCTCC TTAACTATTG CTTCCAAACA
  901 TTTTTGGANA AGANCATGAN TANTGAANTC CCCGANATGT TANCTGAAAT CATCACCNAN
  961 CNNANNNCAA AATATTCAAA TGGAAATATC AAAAAACTTC TGNTTCNTCA AAANCANGGN
1021 NNCNNNNNGN NNNNNNANNN CNNNNNNNNN N
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,mCherry.1 5'
Sequence file: RDB15749_B3Aie.seq check
>D05001C9_B3Ai_2_CMV_Forward_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNT TGNNNNNNTG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGGAC AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC
  181 ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC
  241 CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG
  301 CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG
  361 CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA
  421 TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG
  481 AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC
  541 AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC
  601 TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCCGAGG
  661 TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTGAACA
  721 TCAAGCTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCG
  781 CCGAGGGCCG CGGTACCNAG GGCGAGGANG ACAACATGGC CATCATCAAG GAGTTCATGC
  841 GCTTCAANGT GCACATGGNN GGCTCCGTGA ACGGCCACNA NTTCNAGATC GANGGNGANG
  901 GCGAGGGCCG CCCCTACNAG GGCACCCAGA CCGNCNANNC TGANNGNGAN CNANNGNNNG
  961 NNNCNNNNCN NTCGCNNNGG NNNTNCNTGN NNNNNNANNN CNTGTANGGN NNCNNNNNNN
1021 NNN
//
Primer: RDB15749_R1 (Pr1285)
Region: mCherry.2 5',mCherry.1 3'
Sequence file: RDB15749_B3Aif.seq check
>D05001C9_B3Ai_2_RDB15749_R1_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGNGGCCC TCGGCGCGCT CGTACTGTTC CNCGATGGTG
   61 TAGTCCTCGT TGTGGGAGGT GATGTCCAGC TTGATGTTCA CGTTGTAGGC GCCGGGCAGC
  121 TGCACGGGCT TCTTGGCCTT GTAGGTGGTC TTGACCTCGG CGTCGTAGTG GCCGCCGTCC
  181 TTCAGCTTCA GCCTCTGCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC
  241 TCGGAGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG
  301 TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG
  361 GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG
  421 GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG
  481 CCGTACATGA ACTGAGGGGA CAGGATGTCC CAGGCGAAGG GCAGGGGGCC GCCCTTGGTC
  541 ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC GCCCTCGATC
  601 TCGAACTCGT GGCCGTTCAC GGAGCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG
  661 ATGATGGCCA TGTTGTCCTC CTCGCCCTTG GTACCGCGGC CCTCGGCGCG CTCGTACTGT
  721 TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA GCTTGATGTT CACGTTGTAG
  781 GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTANGTGG TCTTGACCTC NGCGTCGTAN
  841 TGNCCGCCGT CCTTCANCTT CAGCCTCTGC TTGATCTCGC CCTTCANGGC GCCGTCCTCN
  901 GGGTACATCC GCTCGGANGA NGNCTNCCAG CCNATGGNCN TCNNCTGNNT TANNGGGNCG
  961 TCNNANGGGA AGTNGNNGCC GCGNAGCTTC NCNNNGNANA NGNNNNCNNN NNCCNGNNNN
1021 NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB15749_B3Aig.seq check
>D05001C9_B3Ai_2_SV40pro_ori_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN CCTCGGCCTC TGANCTATTC CNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
  121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
  181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
  241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
  301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
  361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
  421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
  481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
  541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTNGTCGA TCAGGATGAT CTGGACGAAG
  601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TNNNNNNNCT CAAGGCGAGC ATGCCCGACG
  661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
  721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCAGGACA
  781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAN AGCTTGGCGG CGAATGNGCT GACCGCTTCC
  841 TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCANCGCAT CGCCTTCTAT CGCCTTCTTG
  901 ACNANTNCNN CTGANCGGGN NNCTGGGGGT NCGAAATGAN CGANCNAGCG ACGCCCNNNC
  961 TGNCNTCACG ANNTTNCNAN TNNNNNNNCN NNNNTNNNNN NAANNNNNGG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tiwari, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]