Please review the QC test results indicated by icon below as well as clone information before placing your order.

pmCherry2-hGRalpha1

Expression vector of human GR alpha1

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017).
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017)。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15749_B3Aip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40polyA-R (Pr0109) Region: insert 3' Sequence file: RDB15749_B3Aia.seq
>D05001C9_B3Ai_2_SV40polyA-R_A06_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TCTANNAATG TGGTATGGCT GATTATGATC    61 AGTTATCTAG ATCNGGTGGA TCCCGTGCTT TTGATGAAAC AGAAGTTTTT TGATATTTCC   121 ATTTGAATAT TTTGGTATCT GATTGGTGAT GATTTCAGCT AACATCTCGG GGAATTCAAT   181 ACTCATGGTC TTATCCAAAA ATGTTTGGAA GCAATAGTTA AGGAGATTTT CAACCACTTC   241 ATGCATAGAA TCCAAGAGTT TTGTCAGTTG ATAAAACCGC TGCCAGTTCT GGCTGGAGTT   301 TCCTTCCCTC TTGACAATGG CTTTTCCTAG CTCTTTGATG TAGGTCATTC TAATTTCATC   361 AAATAGCTCT TGGCTCTTCA GACCGTCCTT AGGAACTGAA GAGAGAAGCA GTAAGGTTTT   421 CATACAGAGA TACTCTTCAT AAGATACCTG AAGCCTGTGT AACTCAGAGG AAACATACAG   481 CATGTGTTTA CATTGGTCGT ACATGCAGGG TAGAGTCATT CTCTGCTCAT TAATAATCAG   541 ATCAGGAGCA AAACACAGCA GGTTTGCACT TGATTGTCTA TATGATCTCC ACCCCAGAGC   601 AAATGCCATA AGAAACATCC AGGAGTACTG CAGTAGGGTC ATTTGGTCAT CCAGGTGTAA   661 GTTCCTGAAA CCTGGTATTG CCTTTGCCCA TTTCACTGCT GCAATCACTT GCCGCCCTCC   721 TAACATGTTG AGCGTAGTCA TGATCCTCCA AGTTGAGTCT GGAACAGAGC TATCATATCC   781 TGCATATAAC ACTTCAGGTT CAATAACCTC CAACAGNGAC ACCAGGGTAN GGGTGAGTTG   841 TGGTAACGTT GCANGAACTA TTGTTTTGNN NCCNGGATTT TCANANGNTT CNTGTGAGNN   901 TCCTGTAGNG NNCTGCTGAN NNNCTTTTAT TTTTTTTCTT NGNTTTTCNA GNTNNNNNGN   961 NCATTCNNGC CNGAANANNT TTTNCGANAN NNGNCNNGNT GGGNNNNTTT TNNNNNNANT 1021 TTNNNNNNNN ANGNNNNNNN TNNNNNNNNN N //
Primer: hGRalpha-N_R (Pr0534) Region: insert 5',mCherry.2 3' Sequence file: RDB15749_B3Aib.seq
>D05001C9_B3Ai_2_hGRalpha-N_R_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNTNNGNNN CTGNNTGAGA AGCGACAGCC NGTGAGGGTG AAGACGCAGA    61 AACCTTCACA GTAGCTCCTC CTCTTAGGGT TTTATAGAAG TCCATCACAT CTCCCCTCTC   121 CTGAGCAAGC ACACTGCTGG GGTTTTCTTC TCTACCAGGA GTTAATGATT CTTTGGAGTC   181 CATCAGTGGA GCTCGAGATC TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGCCGGTGGA   241 GTGGCGGCCC TCGGCGCGCT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT   301 GATGTCCAGC TTGATGTTCA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT   361 GTAGGTGGTC TTGACCTCGG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT   421 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC   481 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC   541 CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC   601 GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA   661 GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA   721 CAGGATGTCC CAGGCGAAGG GCAGGGGGCC GCCCTTGGTC ACCTTCAGCT TGGCGGTCTG   781 GGTGCCCTCG TANGGGCGGN CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC   841 GGANCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGANGGNNN TGTTGTCCTC   901 CTCGCCCTNG GTANCGCGGC CNTCNGCGCG CTCGNACTGT TCCNCGATGG NGNANNCCNC   961 NNNGTGGGAN GNGNTGNNNA GNNNGNTGNT CACNNNGNNN NNNNNGNNNN CTGNNNNNNN 1021 NNN //
Primer: hGRalpha-M_F (Pr0533) Region: insert mid Sequence file: RDB15749_B3Aic.seq
>D05001C9_B3Ai_2_hGRalpha-M_F_C06_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNAAGGNN AATGGAGNNC TGGTTTTGTC AAGCCCCAGT    61 AATGTAACAC TGCCCCAAGT GAAAACAGAA AAAGAAGATT TCATCGAACT CTGCACCCCT   121 GGGGTAATTA AGCAAGAGAA ACTGGGCACA GTTTACTGTC AGGCAAGCTT TCCTGGAGCA   181 AATATAATTG GTAATAAAAT GTCTGCCATT TCTGTTCATG GTGTGAGTAC CTCTGGAGGA   241 CAGATGTACC ACTATGACAT GAATACAGCA TCCCTTTCTC AACAGCAGGA TCAGAAGCCT   301 ATTTTTAATG TCATTCCACC AATTCCCGTT GGTTCCGAAA ATTGGAATAG GTGCCAAGGA   361 TCTGGAGATG ACAACTTGAC TTCTCTGGGG ACTCTGAACT TCCCTGGTCG AACAGTTTTT   421 TCTAATGGCT ATTCAAGCCC CAGCATGAGA CCAGATGTAA GCTCTCCTCC ATCCAGCTCC   481 TCAACAGCAA CAACAGGACC ACCTCCCAAA CTCTGCCTGG TGTGCTCTGA TGAAGCTTCA   541 GGATGTCATT ATGGAGTCTT AACTTGTGGA AGCTGTAAAG TTTTCTTCAA AAGAGCAGTG   601 GAAGGACAGC ACAATTACCT ATGTGCTGGA AGGAATGATT GCATCATCGA TAAAATTCGA   661 AGAAAAAACT GCCCAGCATG CCGCTATCGA AAATGTCTTC AGGCTGGAAT GAACCTGGAA   721 GCTCGAAAAA CAAAGAAAAA AATAAAAGGA ATTCAGCANG NNACTACAGG AGTCTCACAA   781 GAAACCTCTG ANNATCCTGN TAACAAAACA ATAGTTCCTG CAACGTTACC ACAACTCACC   841 CCTACCCTGG TGTCACTGTT GGANGNTATT GAANNTGAAG TGNTATATGC ANGANANGAT   901 AGCTCTGNTC CNGANTCNAC TNGGANGANC ATGANTACNC TCNANATGNN ANGANGGNGG   961 NNAGNGATTG CAGCANTGAA ATGGNNAAAN GNNNTACCNN NTTTCNNNNN TTANNNNNNG 1021 NNNGANNNAN NGNNCNNNNN NNNNN //
Primer: RDB15749_F1 (Pr1284) Region: insert mid Sequence file: RDB15749_B3Aid.seq
>D05001C9_B3Ai_2_RDB15749_F1_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCNGTG GNAGGNNAGC ACAATTACCT ATGTGCTGGA    61 AGGAATGATT GCATCATCGA TAAAATTCGA AGAAAAAACT GCCCAGCATG CCGCTATCGA   121 AAATGTCTTC AGGCTGGAAT GAACCTGGAA GCTCGAAAAA CAAAGAAAAA AATAAAAGGA   181 ATTCAGCAGG CCACTACAGG AGTCTCACAA GAAACCTCTG AAAATCCTGG TAACAAAACA   241 ATAGTTCCTG CAACGTTACC ACAACTCACC CCTACCCTGG TGTCACTGTT GGAGGTTATT   301 GAACCTGAAG TGTTATATGC AGGATATGAT AGCTCTGTTC CAGACTCAAC TTGGAGGATC   361 ATGACTACGC TCAACATGTT AGGAGGGCGG CAAGTGATTG CAGCAGTGAA ATGGGCAAAG   421 GCAATACCAG GTTTCAGGAA CTTACACCTG GATGACCAAA TGACCCTACT GCAGTACTCC   481 TGGATGTTTC TTATGGCATT TGCTCTGGGG TGGAGATCAT ATAGACAATC AAGTGCAAAC   541 CTGCTGTGTT TTGCTCCTGA TCTGATTATT AATGAGCAGA GAATGACTCT ACCCTGCATG   601 TACGACCAAT GTAAACACAT GCTGTATGTT TCCTCTGAGT TACACAGGCT TCAGGTATCT   661 TATGAAGAGT ATCTCTGTAT GAAAACCTTA CTGCTTCTCT CTTCAGTTCC TAAGGACGGT   721 CTGAAGAGCC AAGAGCTATT TGATGAAATT AGAATGACCT ACATCAAAGA GCTAGGAAAA   781 GCCATTGTCA AGAGGGAANG AAACTCCAGC CAGAACTGGC AGCGGTTTTA TCAACTGACA   841 AAACTCTTGN ATTCTATGCA TGAAGTGGTT GAAAATCTCC TTAACTATTG CTTCCAAACA   901 TTTTTGGANA AGANCATGAN TANTGAANTC CCCGANATGT TANCTGAAAT CATCACCNAN   961 CNNANNNCAA AATATTCAAA TGGAAATATC AAAAAACTTC TGNTTCNTCA AAANCANGGN 1021 NNCNNNNNGN NNNNNNANNN CNNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro,mCherry.1 5' Sequence file: RDB15749_B3Aie.seq
>D05001C9_B3Ai_2_CMV_Forward_E06_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNCNNT TGNNNNNNTG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG   121 TGAGCAAGGG CGAGGAGGAC AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC   181 ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC   241 CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG   301 CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG   361 CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA   421 TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG   481 AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC   541 AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC   601 TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCCGAGG   661 TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTGAACA   721 TCAAGCTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCG   781 CCGAGGGCCG CGGTACCNAG GGCGAGGANG ACAACATGGC CATCATCAAG GAGTTCATGC   841 GCTTCAANGT GCACATGGNN GGCTCCGTGA ACGGCCACNA NTTCNAGATC GANGGNGANG   901 GCGAGGGCCG CCCCTACNAG GGCACCCAGA CCGNCNANNC TGANNGNGAN CNANNGNNNG   961 NNNCNNNNCN NTCGCNNNGG NNNTNCNTGN NNNNNNANNN CNTGTANGGN NNCNNNNNNN 1021 NNN //
Primer: RDB15749_R1 (Pr1285) Region: mCherry.2 5',mCherry.1 3' Sequence file: RDB15749_B3Aif.seq
>D05001C9_B3Ai_2_RDB15749_R1_F06_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGNGGCCC TCGGCGCGCT CGTACTGTTC CNCGATGGTG    61 TAGTCCTCGT TGTGGGAGGT GATGTCCAGC TTGATGTTCA CGTTGTAGGC GCCGGGCAGC   121 TGCACGGGCT TCTTGGCCTT GTAGGTGGTC TTGACCTCGG CGTCGTAGTG GCCGCCGTCC   181 TTCAGCTTCA GCCTCTGCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC   241 TCGGAGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG   301 TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG   361 GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG   421 GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG   481 CCGTACATGA ACTGAGGGGA CAGGATGTCC CAGGCGAAGG GCAGGGGGCC GCCCTTGGTC   541 ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC GCCCTCGATC   601 TCGAACTCGT GGCCGTTCAC GGAGCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG   661 ATGATGGCCA TGTTGTCCTC CTCGCCCTTG GTACCGCGGC CCTCGGCGCG CTCGTACTGT   721 TCCACGATGG TGTAGTCCTC GTTGTGGGAG GTGATGTCCA GCTTGATGTT CACGTTGTAG   781 GCGCCGGGCA GCTGCACGGG CTTCTTGGCC TTGTANGTGG TCTTGACCTC NGCGTCGTAN   841 TGNCCGCCGT CCTTCANCTT CAGCCTCTGC TTGATCTCGC CCTTCANGGC GCCGTCCTCN   901 GGGTACATCC GCTCGGANGA NGNCTNCCAG CCNATGGNCN TCNNCTGNNT TANNGGGNCG   961 TCNNANGGGA AGTNGNNGCC GCGNAGCTTC NCNNNGNANA NGNNNNCNNN NNCCNGNNNN 1021 NNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB15749_B3Aig.seq
>D05001C9_B3Ai_2_SV40pro_ori_F_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCN CCTCGGCCTC TGANCTATTC CNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA   121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG   181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG   241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC   301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC   361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG   421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC   481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA   541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTNGTCGA TCAGGATGAT CTGGACGAAG   601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TNNNNNNNCT CAAGGCGAGC ATGCCCGACG   661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG   721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCAGGACA   781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAN AGCTTGGCGG CGAATGNGCT GACCGCTTCC   841 TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCANCGCAT CGCCTTCTAT CGCCTTCTTG   901 ACNANTNCNN CTGANCGGGN NNCTGGGGGT NCGAAATGAN CGANCNAGCG ACGCCCNNNC   961 TGNCNTCACG ANNTTNCNAN TNNNNNNNCN NNNNTNNNNN NAANNNNNGG NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.12.13

GNP_filter3_RDBDEP_html_231213.pl