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pEGFP-hGRalpha

Expression vector of human GR alpha1.

Catalog number RDB15750
Resource name pEGFP-hGRalpha
Clone info. Expression vector of human glucocorticoid receptor (GR alpha1), fused with EGFP at N-terminal, CMV promoter, see Fig. S1 of Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017.
Comment Expression was confirmed by the depositor with fluorescence cross-correlation spectroscopy (FCCS) and microscopic observation.
Vector backbone pEGFP-C1 (plasmid)
Gene/insert name human NR3C1 cDNA
Depositor|Developer Kinjo, Masataka |
Other clones in our bank

External Database
human NR3C1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017)。
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          RDB15750 pEGFP-hGRalpha DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEGFP-hGRalpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15750).

          Reference section:

          Tiwari, M., Oasa, S., Yamamoto, J., Mikuni, S., Kinjo, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15750_B3Aip1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP,insert 5'
          Sequence file: RDB15750_B3Aia.seq check
          >D05001D1_B3Ai_3_EGFP-C2_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NCNCNTGGTC CTGCTGGANT TCGTGANCGC CGCCGGGNTC
             61 ACTCTCGGCA TGGNCGAGCT GTACAAGTCC GGACTCAGAT CTCGAGCTCC ACTGATGGAC
            121 TCCAAAGAAT CATTAACTCC TGGTAGAGAA GAAAACCCCA GCAGTGTGCT TGCTCAGGAG
            181 AGGGGAGATG TGATGGACTT CTATAAAACC CTAAGAGGAG GAGCTACTGT GAAGGTTTCT
            241 GCGTCTTCAC CCTCACTGGC TGTCGCTTCT CAATCAGACT CCAAGCAGCG AAGACTTTTG
            301 GTTGATTTTC CAAAAGGCTC AGTAAGCAAT GCGCAGCAGC CAGATCTGTC CAAAGCAGTT
            361 TCACTCTCAA TGGGACTGTA TATGGGAGAG ACAGAAACAA AAGTGATGGG AAATGACCTG
            421 GGATTCCCAC AGCAGGGCCA AATCAGCCTT TCCTCGGGGG AAACAGACTT AAAGCTTTTG
            481 GAAGAAAGCA TTGCAAACCT CAATAGGTCG ACCAGTGTTC CAGAGAACCC CAAGAGTTCA
            541 GCATCCACTG CTGTGTCTGC TGCCCCCACA GAGAAGGAGT TTCCAAAAAC TCACTCTGAT
            601 GTATCTTCAG AACAGCAACA TTTGAAGGGC CAGACTGGCA CCAACGGTGG CAATGTGAAA
            661 TTGTATACCA CAGACCAAAG CACCTTTGAC ATTTTGCAGG ATTTGGAGTT TTCTTCTGGG
            721 TCCCCAGGTA AAGAGACGAA TGAGAGTCCT TGGAGATCAG ACCTGTTGAT AGATGAAAAC
            781 TGTTTGCTTT CTCCTCTGGC GGGAGAAGAN GATTCATTCC TTTTGGAANG AAACTCGAAT
            841 GANGANTGCA AGCCTCTCAT TTTACCGGAN ACTAAACCNN AAATTAAGGA TNNTGGAGAT
            901 CTGGTTTTGT CNAGCCCCAN NAANGTAACA CTGCCCCAAG NGAAAACAGA AAAANAANAN
            961 TTCNTCGANN NCTGCANCCN NNGGNNNNTT NANCNNNANA ANNTGGGNNN ANNTNNNNNN
          1021 CNNNNNNNCN TNNNNNNNNN N
          //
          Primer: SV40polyA-R (Pr0109)
          Region: insert 3'
          Sequence file: RDB15750_B3Aib.seq check
          >D05001D1_B3Ai_3_SV40polyA-R_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNAANANC TCTACNAATG TGGTATGGCT GATTATGATC
             61 AGTTATCTAG ATCCGGTGGA TCCCGTGCTT TTGATGAAAC AGAAGTTTTT TGATATTTCC
            121 ATTTGAATAT TTTGGTATCT GATTGGTGAT GATTTCAGCT AACATCTCGG GGAATTCAAT
            181 ACTCATGGTC TTATCCAAAA ATGTTTGGAA GCAATAGTTA AGGAGATTTT CAACCACTTC
            241 ATGCATAGAA TCCAAGAGTT TTGTCAGTTG ATAAAACCGC TGCCAGTTCT GGCTGGAGTT
            301 TCCTTCCCTC TTGACAATGG CTTTTCCTAG CTCTTTGATG TAGGTCATTC TAATTTCATC
            361 AAATAGCTCT TGGCTCTTCA GACCGTCCTT AGGAACTGAA GAGAGAAGCA GTAAGGTTTT
            421 CATACAGAGA TACTCTTCAT AAGATACCTG AAGCCTGTGT AACTCAGAGG AAACATACAG
            481 CATGTGTTTA CATTGGTCGT ACATGCAGGG TAGAGTCATT CTCTGCTCAT TAATAATCAG
            541 ATCAGGAGCA AAACACAGCA GGTTTGCACT TGATTGTCTA TATGATCTCC ACCCCAGAGC
            601 AAATGCCATA AGAAACATCC AGGAGTACTG CAGTAGGGTC ATTTGGTCAT CCAGGTGTAA
            661 GTTCCTGAAA CCTGGTATTG CCTTTGCCCA TTTCACTGCT GCAATCACTT GCCGCCCTCC
            721 TAACATGTTG AGCGTAGTCA TGATCCTCCN AGTTGAGTCT GGAACAGAGC TATCATATCC
            781 TGCATATAAC ACTTCANGTT CAATAACCTC CAACAGTGAC ACCAGGGTAG GGGTGAGTTG
            841 TGGTAACGTT GCANGAACTA TTGTTTTGTT ACCAGGANTT TCANAGGTTT CTTGTGAGAN
            901 TCCNGTANNG GCCNGCTGAN TTNCTTTTAN TTTTTNCTTN GTTTTTCGAG CTTNNNNGTT
            961 CNTTNNNGCC NNAAGANNTT TTCNANAGCG NNATGNNNGN NNNTTTTTTN NNCNANTTTN
          1021 NNNNNNNNN
          //
          Primer: hGRalpha-M_F (Pr0533)
          Region: insert mid
          Sequence file: RDB15750_B3Aic.seq check
          >D05001D1_B3Ai_3_hGRalpha-M_F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNAAA NTTAAGGNNA ANGGAGANCT GGTTTTGTCA AGCCCCAGTA
             61 ATGTAACACT GCCCCAAGTG AAAACAGAAA AAGAAGATTT CATCGAACTC TGCACCCCTG
            121 GGGTAATTAA GCAAGAGAAA CTGGGCACAG TTTACTGTCA GGCAAGCTTT CCTGGAGCAA
            181 ATATAATTGG TAATAAAATG TCTGCCATTT CTGTTCATGG TGTGAGTACC TCTGGAGGAC
            241 AGATGTACCA CTATGACATG AATACAGCAT CCCTTTCTCA ACAGCAGGAT CAGAAGCCTA
            301 TTTTTAATGT CATTCCACCA ATTCCCGTTG GTTCCGAAAA TTGGAATAGG TGCCAAGGAT
            361 CTGGAGATGA CAACTTGACT TCTCTGGGGA CTCTGAACTT CCCTGGTCGA ACAGTTTTTT
            421 CTAATGGCTA TTCAAGCCCC AGCATGAGAC CAGATGTAAG CTCTCCTCCA TCCAGCTCCT
            481 CAACAGCAAC AACAGGACCA CCTCCCAAAC TCTGCCTGGT GTGCTCTGAT GAAGCTTCAG
            541 GATGTCATTA TGGAGTCTTA ACTTGTGGAA GCTGTAAAGT TTTCTTCAAA AGAGCAGTGG
            601 AAGGACAGCA CAATTACCTA TGTGCTGGAA GGAATGATTG CATCATCGAT AAAATTCGAA
            661 GAAAAAACTG CCCAGCATGC CGCTATCGAA AATGTCTTCA GGCTGGAATG AACCTGGAAG
            721 CTCGAAAAAC AAAGAAAAAA ATAAAAGGAA TTCAGCAGGN CACTACAGGA GTCTCACAAG
            781 AAACCTCTGA AAATCCTGGT AACAAAACAA TAGTTCCTGC NACGTTACCA CAACTCACCC
            841 CTACNCTGGN GTCNCTGTTG NANGTTATTG AANCTGAAGT GNTATATGCA NGATATGATA
            901 GCTCTGTTNC NNANTCAACT TGGANGANCA TGANTACGCT CAACATGTTA NGANGGCGGN
            961 NNGTGANNGC ANCANTGAAA TGGGCAAANG NNATNNNAGN TTTCNNNNNN TNNNNNNNNN
          1021 N
          //
          Primer: RDB15749_F1 (Pr1284)
          Region: insert mid
          Sequence file: RDB15750_B3Aid.seq check
          >D05001D1_B3Ai_3_RDB15749_F1_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNANNA NCNGTGGAAG GNNAGCACAA TTACCTATGT GCTGGAAGGA
             61 ATGATTGCAT CATCGATAAA ATTCGAAGAA AAAACTGCCC AGCATGCCGC TATCGAAAAT
            121 GTCTTCAGGC TGGAATGAAC CTGGAAGCTC GAAAAACAAA GAAAAAAATA AAAGGAATTC
            181 AGCAGGCCAC TACAGGAGTC TCACAAGAAA CCTCTGAAAA TCCTGGTAAC AAAACAATAG
            241 TTCCTGCAAC GTTACCACAA CTCACCCCTA CCCTGGTGTC ACTGTTGGAG GTTATTGAAC
            301 CTGAAGTGTT ATATGCAGGA TATGATAGCT CTGTTCCAGA CTCAACTTGG AGGATCATGA
            361 CTACGCTCAA CATGTTAGGA GGGCGGCAAG TGATTGCAGC AGTGAAATGG GCAAAGGCAA
            421 TACCAGGTTT CAGGAACTTA CACCTGGATG ACCAAATGAC CCTACTGCAG TACTCCTGGA
            481 TGTTTCTTAT GGCATTTGCT CTGGGGTGGA GATCATATAG ACAATCAAGT GCAAACCTGC
            541 TGTGTTTTGC TCCTGATCTG ATTATTAATG AGCAGAGAAT GACTCTACCC TGCATGTACG
            601 ACCAATGTAA ACACATGCTG TATGTTTCCT CTGAGTTACA CAGGCTTCAG GTATCTTATG
            661 AAGAGTATCT CTGTATGAAA ACCTTACTGC TTCTCTCTTC AGTTCCTAAG GACGGTCTGA
            721 AGAGCCAAGA GCTATTTGAT GAAATTAGAA TGACCTACAT CAAAGAGCTA GGAAAAGCCA
            781 TTGTCAAGAG GGAAGGAAAC TCCAGCCAGA ACTGGCAGCG GTTTTATCAA CTGACAAAAC
            841 TCTTGGATTC TATGCATGAA GTGGTTGAAA NTCTCCTTAA CTATTGCTTN CAAACNTTTT
            901 TGGATAANAN CATGANTATT GAATTCCCNG ANNNGNTANC TGAAATCATC ACCNATCNNA
            961 NACNNAAATA NNCAANTNGN AANNATCNAA AANNNNNNGN TNNNTCNAAA NCNNNGNNNN
          1021 NNNNNGNNNN NNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,EGFP
          Sequence file: RDB15750_B3Aie.seq check
          >D05001D1_B3Ai_3_CMV_Forward_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNCNNNN TTGNNGCNNN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG
            121 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
            181 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
            241 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
            301 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
            361 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
            421 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
            481 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
            541 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
            601 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
            661 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
            721 CTGAGCACCC AGTCCGCCCT GAGCAAAGAN CCCAACGAGA AGCGCGATCA CATGGTCCTG
            781 CTGGAGTTCG TGANCGCCGC CGGGATCACT CTCGGCATGG ACGANCTGTA CAAGTCCGGA
            841 NTCAGATCTC GAGCTCCACT GATGGANTCC NAAGAATCAT TAACTCNNGG TAGANAANAA
            901 AANCCNAGCA GNGNGCTTGC TCNNNNAGGG GANANGTGNT GGANTTCTAT NAAANCCTNA
          961 NANGANNNNC TNNNGNGANN NNTNNNGNNN NTNCNCCNNN NNNNGNNNNN NNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV 40 pro_ori,NeoR_KanR
          Sequence file: RDB15750_B3Aif.seq check
          >D05001D1_B3Ai_3_SV40pro_ori_F_F01_16_ABI24.ab1
              1 NNNNNNNNNN NGNNGCNGCC TCNNCCTCTG ANCTATTCCN GAAGTAGTGA GGAGGCTTTT
             61 TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG
            121 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
            181 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
            241 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA
            301 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC
            361 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
            421 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
            481 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
            541 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG
            601 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC
            661 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC
            721 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA TCAGGACATA
            781 GCGTTGGCTA CNCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA NCGCTTCCTC
            841 GTGCTTTACN GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CNNTCTTGNN
            901 NANTNCTTCT GANCGGGNNT CNNGGGTTCG AANTGNNNNA NCNANCGNNN CCCNNNNNGN
            961 NNTCNCNANN NTNCNANTNN NNNGNCNNNN NTNNANNAAN NNNNGGNNTT CNNNNNNNNT
          1021 TTNCNNGGNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tiwari, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]

          2024.03.26

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