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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-hGRalpha (#RDB15750)

Expression vector of human GR alpha1.

Clone info. Expression vector of human glucocorticoid receptor (GR alpha1), fused with EGFP at N-terminal, CMV promoter, see Fig. S1 of Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017.
Comment Expression was confirmed by the depositor with fluorescence cross-correlation spectroscopy (FCCS) and microscopic observation.
Vector backbone pEGFP-C1 (plasmid)
Gene/insert name human NR3C1 cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017).
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15750 pEGFP-hGRalpha DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-hGRalpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15750).

Reference section:

Tiwari, M., Oasa, S., Yamamoto, J., Mikuni, S., Kinjo, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15750_B3Aip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP,insert 5'
Sequence file: RDB15750_B3Aia.seq check
>D05001D1_B3Ai_3_EGFP-C2_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NCNCNTGGTC CTGCTGGANT TCGTGANCGC CGCCGGGNTC
   61 ACTCTCGGCA TGGNCGAGCT GTACAAGTCC GGACTCAGAT CTCGAGCTCC ACTGATGGAC
  121 TCCAAAGAAT CATTAACTCC TGGTAGAGAA GAAAACCCCA GCAGTGTGCT TGCTCAGGAG
  181 AGGGGAGATG TGATGGACTT CTATAAAACC CTAAGAGGAG GAGCTACTGT GAAGGTTTCT
  241 GCGTCTTCAC CCTCACTGGC TGTCGCTTCT CAATCAGACT CCAAGCAGCG AAGACTTTTG
  301 GTTGATTTTC CAAAAGGCTC AGTAAGCAAT GCGCAGCAGC CAGATCTGTC CAAAGCAGTT
  361 TCACTCTCAA TGGGACTGTA TATGGGAGAG ACAGAAACAA AAGTGATGGG AAATGACCTG
  421 GGATTCCCAC AGCAGGGCCA AATCAGCCTT TCCTCGGGGG AAACAGACTT AAAGCTTTTG
  481 GAAGAAAGCA TTGCAAACCT CAATAGGTCG ACCAGTGTTC CAGAGAACCC CAAGAGTTCA
  541 GCATCCACTG CTGTGTCTGC TGCCCCCACA GAGAAGGAGT TTCCAAAAAC TCACTCTGAT
  601 GTATCTTCAG AACAGCAACA TTTGAAGGGC CAGACTGGCA CCAACGGTGG CAATGTGAAA
  661 TTGTATACCA CAGACCAAAG CACCTTTGAC ATTTTGCAGG ATTTGGAGTT TTCTTCTGGG
  721 TCCCCAGGTA AAGAGACGAA TGAGAGTCCT TGGAGATCAG ACCTGTTGAT AGATGAAAAC
  781 TGTTTGCTTT CTCCTCTGGC GGGAGAAGAN GATTCATTCC TTTTGGAANG AAACTCGAAT
  841 GANGANTGCA AGCCTCTCAT TTTACCGGAN ACTAAACCNN AAATTAAGGA TNNTGGAGAT
  901 CTGGTTTTGT CNAGCCCCAN NAANGTAACA CTGCCCCAAG NGAAAACAGA AAAANAANAN
  961 TTCNTCGANN NCTGCANCCN NNGGNNNNTT NANCNNNANA ANNTGGGNNN ANNTNNNNNN
1021 CNNNNNNNCN TNNNNNNNNN N
//
Primer: SV40polyA-R (Pr0109)
Region: insert 3'
Sequence file: RDB15750_B3Aib.seq check
>D05001D1_B3Ai_3_SV40polyA-R_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNAANANC TCTACNAATG TGGTATGGCT GATTATGATC
   61 AGTTATCTAG ATCCGGTGGA TCCCGTGCTT TTGATGAAAC AGAAGTTTTT TGATATTTCC
  121 ATTTGAATAT TTTGGTATCT GATTGGTGAT GATTTCAGCT AACATCTCGG GGAATTCAAT
  181 ACTCATGGTC TTATCCAAAA ATGTTTGGAA GCAATAGTTA AGGAGATTTT CAACCACTTC
  241 ATGCATAGAA TCCAAGAGTT TTGTCAGTTG ATAAAACCGC TGCCAGTTCT GGCTGGAGTT
  301 TCCTTCCCTC TTGACAATGG CTTTTCCTAG CTCTTTGATG TAGGTCATTC TAATTTCATC
  361 AAATAGCTCT TGGCTCTTCA GACCGTCCTT AGGAACTGAA GAGAGAAGCA GTAAGGTTTT
  421 CATACAGAGA TACTCTTCAT AAGATACCTG AAGCCTGTGT AACTCAGAGG AAACATACAG
  481 CATGTGTTTA CATTGGTCGT ACATGCAGGG TAGAGTCATT CTCTGCTCAT TAATAATCAG
  541 ATCAGGAGCA AAACACAGCA GGTTTGCACT TGATTGTCTA TATGATCTCC ACCCCAGAGC
  601 AAATGCCATA AGAAACATCC AGGAGTACTG CAGTAGGGTC ATTTGGTCAT CCAGGTGTAA
  661 GTTCCTGAAA CCTGGTATTG CCTTTGCCCA TTTCACTGCT GCAATCACTT GCCGCCCTCC
  721 TAACATGTTG AGCGTAGTCA TGATCCTCCN AGTTGAGTCT GGAACAGAGC TATCATATCC
  781 TGCATATAAC ACTTCANGTT CAATAACCTC CAACAGTGAC ACCAGGGTAG GGGTGAGTTG
  841 TGGTAACGTT GCANGAACTA TTGTTTTGTT ACCAGGANTT TCANAGGTTT CTTGTGAGAN
  901 TCCNGTANNG GCCNGCTGAN TTNCTTTTAN TTTTTNCTTN GTTTTTCGAG CTTNNNNGTT
  961 CNTTNNNGCC NNAAGANNTT TTCNANAGCG NNATGNNNGN NNNTTTTTTN NNCNANTTTN
1021 NNNNNNNNN
//
Primer: hGRalpha-M_F (Pr0533)
Region: insert mid
Sequence file: RDB15750_B3Aic.seq check
>D05001D1_B3Ai_3_hGRalpha-M_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNAAA NTTAAGGNNA ANGGAGANCT GGTTTTGTCA AGCCCCAGTA
   61 ATGTAACACT GCCCCAAGTG AAAACAGAAA AAGAAGATTT CATCGAACTC TGCACCCCTG
  121 GGGTAATTAA GCAAGAGAAA CTGGGCACAG TTTACTGTCA GGCAAGCTTT CCTGGAGCAA
  181 ATATAATTGG TAATAAAATG TCTGCCATTT CTGTTCATGG TGTGAGTACC TCTGGAGGAC
  241 AGATGTACCA CTATGACATG AATACAGCAT CCCTTTCTCA ACAGCAGGAT CAGAAGCCTA
  301 TTTTTAATGT CATTCCACCA ATTCCCGTTG GTTCCGAAAA TTGGAATAGG TGCCAAGGAT
  361 CTGGAGATGA CAACTTGACT TCTCTGGGGA CTCTGAACTT CCCTGGTCGA ACAGTTTTTT
  421 CTAATGGCTA TTCAAGCCCC AGCATGAGAC CAGATGTAAG CTCTCCTCCA TCCAGCTCCT
  481 CAACAGCAAC AACAGGACCA CCTCCCAAAC TCTGCCTGGT GTGCTCTGAT GAAGCTTCAG
  541 GATGTCATTA TGGAGTCTTA ACTTGTGGAA GCTGTAAAGT TTTCTTCAAA AGAGCAGTGG
  601 AAGGACAGCA CAATTACCTA TGTGCTGGAA GGAATGATTG CATCATCGAT AAAATTCGAA
  661 GAAAAAACTG CCCAGCATGC CGCTATCGAA AATGTCTTCA GGCTGGAATG AACCTGGAAG
  721 CTCGAAAAAC AAAGAAAAAA ATAAAAGGAA TTCAGCAGGN CACTACAGGA GTCTCACAAG
  781 AAACCTCTGA AAATCCTGGT AACAAAACAA TAGTTCCTGC NACGTTACCA CAACTCACCC
  841 CTACNCTGGN GTCNCTGTTG NANGTTATTG AANCTGAAGT GNTATATGCA NGATATGATA
  901 GCTCTGTTNC NNANTCAACT TGGANGANCA TGANTACGCT CAACATGTTA NGANGGCGGN
  961 NNGTGANNGC ANCANTGAAA TGGGCAAANG NNATNNNAGN TTTCNNNNNN TNNNNNNNNN
1021 N
//
Primer: RDB15749_F1 (Pr1284)
Region: insert mid
Sequence file: RDB15750_B3Aid.seq check
>D05001D1_B3Ai_3_RDB15749_F1_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNANNA NCNGTGGAAG GNNAGCACAA TTACCTATGT GCTGGAAGGA
   61 ATGATTGCAT CATCGATAAA ATTCGAAGAA AAAACTGCCC AGCATGCCGC TATCGAAAAT
  121 GTCTTCAGGC TGGAATGAAC CTGGAAGCTC GAAAAACAAA GAAAAAAATA AAAGGAATTC
  181 AGCAGGCCAC TACAGGAGTC TCACAAGAAA CCTCTGAAAA TCCTGGTAAC AAAACAATAG
  241 TTCCTGCAAC GTTACCACAA CTCACCCCTA CCCTGGTGTC ACTGTTGGAG GTTATTGAAC
  301 CTGAAGTGTT ATATGCAGGA TATGATAGCT CTGTTCCAGA CTCAACTTGG AGGATCATGA
  361 CTACGCTCAA CATGTTAGGA GGGCGGCAAG TGATTGCAGC AGTGAAATGG GCAAAGGCAA
  421 TACCAGGTTT CAGGAACTTA CACCTGGATG ACCAAATGAC CCTACTGCAG TACTCCTGGA
  481 TGTTTCTTAT GGCATTTGCT CTGGGGTGGA GATCATATAG ACAATCAAGT GCAAACCTGC
  541 TGTGTTTTGC TCCTGATCTG ATTATTAATG AGCAGAGAAT GACTCTACCC TGCATGTACG
  601 ACCAATGTAA ACACATGCTG TATGTTTCCT CTGAGTTACA CAGGCTTCAG GTATCTTATG
  661 AAGAGTATCT CTGTATGAAA ACCTTACTGC TTCTCTCTTC AGTTCCTAAG GACGGTCTGA
  721 AGAGCCAAGA GCTATTTGAT GAAATTAGAA TGACCTACAT CAAAGAGCTA GGAAAAGCCA
  781 TTGTCAAGAG GGAAGGAAAC TCCAGCCAGA ACTGGCAGCG GTTTTATCAA CTGACAAAAC
  841 TCTTGGATTC TATGCATGAA GTGGTTGAAA NTCTCCTTAA CTATTGCTTN CAAACNTTTT
  901 TGGATAANAN CATGANTATT GAATTCCCNG ANNNGNTANC TGAAATCATC ACCNATCNNA
  961 NACNNAAATA NNCAANTNGN AANNATCNAA AANNNNNNGN TNNNTCNAAA NCNNNGNNNN
1021 NNNNNGNNNN NNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP
Sequence file: RDB15750_B3Aie.seq check
>D05001D1_B3Ai_3_CMV_Forward_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNNN TTGNNGCNNN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG
  121 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
  181 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
  241 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
  301 GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG
  361 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
  421 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
  481 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
  541 CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC
  601 ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
  661 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
  721 CTGAGCACCC AGTCCGCCCT GAGCAAAGAN CCCAACGAGA AGCGCGATCA CATGGTCCTG
  781 CTGGAGTTCG TGANCGCCGC CGGGATCACT CTCGGCATGG ACGANCTGTA CAAGTCCGGA
  841 NTCAGATCTC GAGCTCCACT GATGGANTCC NAAGAATCAT TAACTCNNGG TAGANAANAA
  901 AANCCNAGCA GNGNGCTTGC TCNNNNAGGG GANANGTGNT GGANTTCTAT NAAANCCTNA
961 NANGANNNNC TNNNGNGANN NNTNNNGNNN NTNCNCCNNN NNNNGNNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV 40 pro_ori,NeoR_KanR
Sequence file: RDB15750_B3Aif.seq check
>D05001D1_B3Ai_3_SV40pro_ori_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NGNNGCNGCC TCNNCCTCTG ANCTATTCCN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG
  121 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
  181 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
  241 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA
  301 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC
  361 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
  421 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
  481 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
  541 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG
  601 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC
  661 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC
  721 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGANCGCTA TCAGGACATA
  781 GCGTTGGCTA CNCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA NCGCTTCCTC
  841 GTGCTTTACN GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CNNTCTTGNN
  901 NANTNCTTCT GANCGGGNNT CNNGGGTTCG AANTGNNNNA NCNANCGNNN CCCNNNNNGN
  961 NNTCNCNANN NTNCNANTNN NNNGNCNNNN NTNNANNAAN NNNNGGNNTT CNNNNNNNNT
1021 TTNCNNGGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tiwari, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]