Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-hGRalpha/C421G (#RDB15752)

Expression vector of human GR alpha1, DNA-binding-deficient mutant.

Clone info. Expression vector of human glucocorticoid receptor (GR alpha1), DNA-binding-deficient mutant [C421G], fused with EGFP at N-terminal, CMV promoter, see Fig. S1 of Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017.
Comment Expression was confirmed by the depositor with fluorescence cross-correlation spectroscopy (FCCS) and microscopic observation.
Vector backbone plasmid
Gene/insert name human NR3C1 cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tiwari, M. et al., Sci. Rep., 7 (1): 4336, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15752 pEGFP-hGRalpha/C421G Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEGFP-hGRalpha/C421G was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15752).

Reference section:

Tiwari, M., Oasa, S., Yamamoto, J., Mikuni, S., Kinjo, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Tiwari, M., A Quantitative Study of Internal and External Interactions of Homodimeric Glucocorticoid Receptor Using Fluorescence Cross-Correlation Spectroscopy in a Live Cell. Sci. Rep. 7 (1): 4336 (2017). PMID 28659593. [link to RRC of NBRP]