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pGL4.26_0.8kb-Luc

Luciferase reporter of the mouse muscle creatine kinase (MCK) promoter.

Catalog number RDB15785
Resource name pGL4.26_0.8kb-Luc
Clone info. Luciferase reporter of the mouse muscle creatine kinase (MCK) promoter [1-kb fragment (-1.8kb to -0.8 kb relative to transcription start site)] was inserted.
Comment Expression was confirmed by the depositor with luciferase assay.
Vector backbone pGL4.26 (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r (E. coli), Hyg^r (mammalian cells).
Gene/insert name mouse Ckm genomic DNA
Depositor|Developer Kokabu, Shoichiro |
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External Database
mouse Ckm
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kokabu, S. et al. J. Biol. Chem. 292(31): 12885-12894, 2017)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kokabu, S. et al. J. Biol. Chem. 292(31): 12885-12894, 2017)。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB15785 pGL4.26_0.8kb-Luc Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Kokabu, S., The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor. J. Biol. Chem. 292 (31): 12885-12894 (2017). PMID 28607151.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          References

          Original, user report and related articles

          original Kokabu, S., The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor. J. Biol. Chem. 292 (31): 12885-12894 (2017). PMID 28607151.

          2022.05.17

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