Resource data sheet
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pTnT-TUG1 exon1 (#RDB15794)

Expression vector of long non-coding RNA, human TUG1 exon1 for in vitro transcription.

Clone info. Expression vector of long non-coding RNA, human Taurine Upregulated Gene 1 (TUG1) exon1 (1 to 2,132 nucleotides) for in vitro transcription, see Fig. 3e of Katsushima, K. et al. Nat. Commun., 7: 13616, 2016.
Comment Construction was confirmed by the depositor with sequencing analysis.
Vector backbone pTnT (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name human TUG1 cDNA
Depositor|Developer Kondo, Yutaka |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Katsushima, K et al. Nat. Commun. 7: 13616, 2016); (Deguchi, S et al. Oncogene. 36 (32) : 4629 - 4640, 2017)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15794 pTnT-TUG1 exon1 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pTnT-TUG1 exon1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15794).

Reference section:

Katsushima, K., Natsume, A., Ohka, F., Shinjo, K., Hatanaka, A., Ichimura, N., Sato, S., Takahashi, S., Kimura, H., Totoki, Y., Shibata, T., Naito, M., Kim, H.J., Miyata, K., Kataoka, K., Kondo, Y., Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment. Nat. Commun. 7: 13616 (2016). PMID 27922002. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15794_A8Bqp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3' element Reverse
Sequence file: RDB15794_A8Bqa.seq check
1
Primer: f1ori_R3
Sequence file: RDB15794_A8Bqb.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Katsushima, K., Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment. Nat. Commun. 7: 13616 (2016). PMID 27922002. [link to RRC of NBRP]