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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11

Expression vector of sgRNA for targeting human ATM exon11 and human codon-optimized SpCsa9.

Catalog number RDB15816
Resource name pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11
Clone info. Expression vector of sgRNA for targeting human ataxia-telangiectasia mutated (ATM) exon11 and human codon-optimized SpCsa9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (Plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name Human ATM genomic DNA
Depositor|Developer Matsuura, Shinya |
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External Database
Human ATM

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Royba, E et al. Sci. Rep. 7(1): 5996, 2017)
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          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Royba, E et al. Sci Rep. 7(1) : 5996, 2017)。
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          提供依頼書 [Word]
          専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15816 pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pX330-U6-Chimeric_BB-CBh-hSpCas9 sgRNA ATM exon11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15816).

          Reference section:

          Royba, E., Miyamoto, T., Akutsu, S.N., Hosoba, K., Tauchi, H., Kudo, Y., Tashiro, S., Yamamoto, T., Matsuura, S., Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells. Sci. Rep. 7 (1): 5996 (2017). PMID 28729543. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15816_A8Bqp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3' element Reverse
          Sequence file: RDB15816_A8Bqa.seq check
          >D05203A1-5_A8Bq_SV40pA3'elementReverse_A01_01_ABI08.ab1
              1 TCGTTCTAGG GGGGGGCGGG GGCCTATGGA AAACGCCAGC AACGCGGCCT TTTTACGGTT
             61 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTGAGGGCCT ATTTCCCATG ATTCCTTCAT
            121 ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA
            181 CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG
            241 TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG
            301 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGCTGACCAC CAGTATAGTT
            361 CCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA
            421 GTGGCACCGA GTCGGTGCTT TTTTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT
            481 AGTCCGTTTT TAGCGCGTGC GCCAATTCTG CAGACAAATG GCTCTAGAGG TACCCGTTAC
            541 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
            601 AATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
            661 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
            721 TGACGGTAAA TGGCCCGCCT GGCATTGTGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
            781 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT
            841 CTGCTTCACT CTCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT
          901 TTTAATTATT TTGTGCAGCG ATGGGGGGCG GGGGGGGGGG GGGGGGGG
          //
          Primer: RDB13535#1
          Sequence file: RDB15816_A8Bqb.seq check
          >D05203A1-5_A8Bq_RDB13535#1_B01_02_ABI08.ab1
              1 TGGAAATTGG CCCTGAAATC ACTTTTTTTC AGGTTGGACC GGTGCCACCA TGGACTATAA
             61 GGACCACGAC GGAGACTACA AGGATCATGA TATTGATTAC AAAGACGATG ACGATAAGAT
            121 GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC CCAGCAGCCG ACAAGAAGTA
            181 CAGCATCGGC CTGGACATCG GCACCAACTC TGTGGGCTGG GCCGTGATCA CCGACGAGTA
            241 CAAGGTGCCC AGCAAGAAAT TCAAGGTGCT GGGCAACACC GACCGGCACA GCATCAAGAA
            301 GAACCTGATC GGAGCCCTGC TGTTCGACAG CGGCGAAACA GCCGAGGCCA CCCGGCTGAA
            361 GAGAACCGCC AGAAGAAGAT ACACCAGACG GAAGAACCGG ATCTGCTATC TGCAAGAGAT
            421 CTTCAGCAAC GAGATGGCCA AGGTGGACGA CAGCTTCTTC CACAGACTGG AAGAGTCCTT
            481 CCTGGTGGAA GAGGATAAGA AGCACGAGCG GCACCCCATC TTCGGCAACA TCGTGGACGA
            541 GGTGGCCTAC CACGAGAAGT ACCCCACCAT CTACCACCTG AGAAAGAAAC TGGTGGACAG
            601 CACCGACAAG GCCGACCTGC GGCTGATCTA TCTGGCCCTG GCCCACATGA TCAAGTTCCG
            661 GGGCCACTTC CTGATCGAGG GCGACCTGAA CCCCGACAAC AGCGACGTGG ACAAGCTGTT
            721 CATCCAGCTG GTGCAGACCT ACAACCAGCT GTTCGAGGAA AACCCCATCA ACGCCAGCGG
            781 CGTGGACGCC AAGGCCATCC TGTCTGCCAG ACTGAGCAAG AGCAGACGGC TGGAAAATCT
            841 GATCGCCCAG CTGCCCGGCG AGAAGAAGAA TGGCCTGTTC GGAAACCTGA TTGCCCTGAG
            901 CCTGGGCCTG ACCCCCAACT TCAAGAGCAA CTTCGACCTG GCCGAGGATG CCAAACTGCA
            961 GCTGAGCAAG GACACCTACG ACGACGACCC TGGACAACCT GCTGGCCCAG ATCGGCGACC
           1021 AGTTACGCCG GACCTGTTTC TGGCCGCCAG GAACCTGTCC GACGCCCATT CCTGCTGAGC
           1081 GACATCCTGA GAGTGACACG AGATCACAAG ACCCCCCTTG AGCGTCTCTT ATTGAATTCA
          1141 GGAGAATTAC CGAACCGA
          //
          Primer: BGH_rev2
          Sequence file: RDB15816_A8Bqc.seq check
          >D05203A1-5_A8Bq_BGH_rev2_C01_03_ABI08.ab1
              1 CATCAAGGGT CAGGGAAGGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
             61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GGAATTCTTA CTTTTTCTTT TTTGCCTGGC
            121 CGGCCTTTTT CGTGGCCGCC GGCCTTTTGT CGCCTCCCAG CTGAGACAGG TCGATCCGTG
            181 TCTCGTACAG GCCGGTGATG CTCTGGTGGA TCAGGGTGGC GTCCAGCACC TCTTTGGTGC
            241 TGGTGTACCT CTTCCGGTCG ATGGTGGTGT CAAAGTACTT GAAGGCGGCA GGGGCTCCCA
            301 GATTGGTCAG GGTAAACAGG TGGATGATAT TCTCGGCCTG CTCTCTGATG GGCTTATCCC
            361 GGTGCTTGTT GTAGGCGGAC AGCACTTTGT CCAGATTAGC GTCGGCCAGG ATCACTCTCT
            421 TGGAGAACTC GCTGATCTGC TCGATGATCT CGTCCAGGTA GTGCTTGTGC TGTTCCACAA
            481 ACAGCTGTTT CTGCTCATTA TCCTCGGGGG AGCCCTTCAG CTTCTCATAG TGGCTGGCCA
            541 GGTACAGGAA GTTCACATAT TTGGAGGGCA GGGCCAGTTC GTTTCCCTTC TGCAGTTCGC
            601 CGGCAGAGGC CAGCATTCTC TTCCGGCCGT TTTCCAGCTC GAACAGGGAG TACTTAGGCA
            661 GCTTGATGAT CAGGTCCTTT TTCACTTCTT TGTAGCCCTT GGCTTCCAGA AAGTCGATGG
            721 GATTCTTCTC GAAGCTGCTT CTTTCCATGA TGGTGATCCC CAGCAGCTCT TTCACACTCT
            781 TCAGTTTCTT GGACTTGCCC TTTTCCACTT TGGCCACCAC CAGCACAGAA TAGGCCACGG
            841 TGGGGCTGTC GAAGCCGCCG TACTTCTTAG GGTCCCAGTC CTTCTTTCTG GCGATCAGCT
            901 TATCGCTGTT CCTCTTGGGC AGGATAGACT CTTTGCTGAA GCCGCCTGTC TGCACCTCGG
            961 TCTTTTTTCA CGATATTCAC TTGGGGCATG CTCAGCACTT TCCGCACGGT GGGCAAAATC
           1021 CCGGCCCTTT ATCCCACACG AATCTCCCCG GGTTTCGCCG TTTTGTCTCG ATCAGAGACG
           1081 CCTTTCGGAT CTTCGGCCCG GTTGGCCAAG GTATCTCGGT CTGAAAAAGT CCATGGAATG
          1141 ATTGCCTGTT AGAAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Royba, E., Evaluation of ATM heterozygous mutations underlying individual differences in radiosensitivity using genome editing in human cultured cells. Sci. Rep. 7 (1): 5996 (2017). PMID 28729543. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl