Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCSII-CMV-MCS _c-GRAPHIC

Lentiviral expression vector of GRAPHIC probe (LZB).

Sequence (full)	RDB15872zzt01.seq compiled by us
Sequence (partial)	RDB15872pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kinoshita, N. et al., iScience 15: 28-38 (2019)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kinoshita, N. et al., iScience 15: 28-38 (2019)) を引用し、謝辞の表明を必要とする。本件リソースの使用は学術機関での学術研究に限る。 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15872_A8Cdp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C-rev (Pr0026) Region: insert 5',CMV pro.2 Sequence file: RDB15872_A8Cg.seq
>D05101A4-1_A8Cd_EGFP-C-rev_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNNNNGNNG GTCTGGGTGC TCNGGTAGTG GTTGTCGGGC    61 AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG   121 CTGCCGTCCT CGACGTTGTG GCGGACCGTG AAGTTCGCCT TGATGCCGTT CTTTCTAGCC   181 TGCAGCGTGG TGTTATCCTT GGCAACCACG GACACTGCCA AGACCAGCAC AGCGACAGTT   241 GGCAGCATCT CTACCATGGT GGCTCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT   301 TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGANTTGTT   361 ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCNAAAC AAACTCCCAT TGACGTCAAT   421 GGGGTGGAGA CTTGGAAATC CCCGTGANTC NNACCGCTAT CCACGCCCAT TGATGTACTG   481 CCNAAACCGC ATCACCATGG TAATAGCGAN GACTAATACN TNNATGTACT GNCNAGTNNG   541 AAAGTCCCNT ANNGNCNTGT ACTGGGCNTA NNGNCNNGCG GNNNTTTANC NNCATNNANN   601 TCNNNNNGGG GCGTANTTGG CNNNTGANAC NCTNNNNGNA CNNNNNNNNN GGCANNTNAN 661 NNNAAANNNN NNNNCNNNNG NNNNCNNNGN NAANNNNNNN NNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3' Sequence file: RDB15872_A8Ch.seq
>D05101A4_1_A8Cd_PRE_rev_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNCA TAGTTAAGAA TACCAGTCAA TCTTTCACAA    61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG   121 ATCCGCGGCC GCTGCAGAAT TCTCGAGACC GGTAGCGCTA GATTACAGAG AAATGAAGTC   181 CAGGGCTTGG AGGAGGGAGA GGGAAAGCAG CAGCAGCAGC ATCCAGGATG TGTTCTGAAC   241 CAGCAGGCTT ATGCCGCCAT TCTCGAGTTG GTAGCTAGAC GATCCGCCAC CGCCAGAGCC   301 ACCTCCGCCT GAACCGCCTC CACCGCTAGA CGATCCGCCA CCGCCAGAGC CACCTCCGCC   361 TGAACCGCCT CCACCGCTAG ACGATCCGCC ACCGCCAGAG CCACCTCCGC CTGAACCGCC   421 TCCACCGCTA GCCTTCTGGG CCAGCTTCTT CTTCAGGGCC TGGAGCTTCC ACTTGAGCTG   481 GGCGTTCTTC TTCTTCAGGG CTTGCAGCTT CTTCTTGAGC TGGGCCATGG TGGCGCTAGC   541 CGATCCGCCA CCGCCAGAGC CACCTCCGCC TGAACCGCCT CCACCGGATC TCTTGTACAG   601 CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGN NCATGTGATC   661 GCGCTTCTCG TTGGGGTCTT TGCTCANGAC GGTCTGGGTG CTCAGGTAGT GNTTGTCGGG   721 CAGCAGCACG GGGCCGTCGC CNATGGGGGT GTTCTGCTGG TAGTGGTCGG CGANCTGCAC   781 GCTGCCGTCC TCNACGTTGT GNCGGANCGN GAAGTTCGCC TTGATGCCGT TCTTTCTANC   841 CNGCANNGNG GNGNTATNCT NNGNNANNNN GGNNNCNGNC NNNANCNGCA CAGNGACNGT 901 NNNNAGCATN NNNACNNNNN NNGNCTNNNN NNNNNN //
Primer: CSII-CMV5' (Pr0021) Region: insert 3' Sequence file: RDB15872_A8Ci.seq
>D05101A4_A8Cd_1_CSII-CMV5'_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC TGCCNACTGT CGCTGTGCTG GTCTTGGCAG    61 TGTCCGTGGT TGCCAAGGNT AACACCACGC TGCAGGCTAG AAAGAACGGC ATCAAGGCGA   121 ACTTCACGGT CCGCCACAAC GTCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC   181 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC   241 AGACCGTCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG   301 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGAGATCC GGTGGAGGCG   361 GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG GATCGGCTAG CGCCACCATG GCCCAGCTCA   421 AGAAGAAGCT GCAAGCCCTG AAGAAGAAGA ACGCCCAGCT CAAGTGGAAG CTCCAGGCCC   481 TGAAGAAGAA GCTGGCCCAG AAGGCTAGCG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG   541 GCGGTGGCGG ATCGTCTAGC GGTGGAGGCG GTTCAGGCGG AGGTGGCTCT GGCGGTGGCG   601 GATCGTCTAG CGGTGGAGGC GGTTCAGGCG GAGGTGGCTC TGGCGGTGGC GGATCGTCTA   661 GCTACCAACT CGAGAATGGC GGCATAAGCC TGCTGGTTCA GAACACATCC TGGATGCTGC   721 TGCTGCTGCT TTCCCTCTCC CTCCTCCAAG CCCTGGACTT CATTTCTCTG TAATCTAGCG   781 CTACCGGTCT CGAGAATTCT GCAGCGGCCG CGGNTCCTCT AGAGTTAACA TCGANGGATC   841 AAGCTTATCG ATAATCAACC TCTGGANTAC AAAATTTGTG AAAGANTGAC TNNNATTCTT   901 AACTATGTNG CTNCTTTTAC GCTATGNNNA TANNCTGCNT TNANGNNNTT GNATCANGNT 961 ATNNNNTNNC CNNTATGNNT TTNNATTTTT NNNNNNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi,5' LTR,CMV pro.1,CMV en.1 Sequence file: RDB15872_A8Cj.seq
>D05101A4_A8Cd_1_HIV-B_R_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTT TGGCGNANTC NCCNGTCGCC GCCCCTCGCC    61 TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA GAGAGCTCCT CTGGTTTCCC   121 TTTCGCTTTC AAGTCCCTGT TCGGGCGCCA CTGCTAGAGA TTTTCCACAC TGACTAAAAG   181 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   241 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   301 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGG CAAAACGCGC TGCTTATATA   361 GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA   421 TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG   481 GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA   541 CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC   601 CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT   661 AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA   721 ATACTCCACC CATTGACGTN NATGGAAAGT CCCTATTGNN GTTACTATGG GAACATACGT   781 CATTATTGAC GTCNATGGGC GGGGGTCGTT GGGCGGTCAG NNAGGCGGGC CATTTACCGT   841 AAGTTATGTA ACGCGNANCT CCNTATATGG GNCTATGNAN TAANGACCCC NTAATNNATN 901 ACTANNAANN ACNNNNCAAT AATCNNNNNN NNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,3' LTR,PRE Sequence file: RDB15872_A8Ck.seq
>D05101A4_A8Cd_1_BGH_rev2_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG CANNNACAGN NGGCTGGCAA CTAGAAGGCA    61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA   121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA   181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC   241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG   301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG   361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT   421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA   481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG   541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC   601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA   661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA   721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT   781 CGACGGTATC GATGCGGGGA NGCGGCCCAA AGGGAGATCC GACTCGTCTG ANGGCGAANG   841 CGAANANNCG GAANANGNCG CAGANCNNGC AGCANNNCGC GGGAAGGAAG GTCCNCTGGA   901 TNNAGGGNNG AAGGGNNGTA NCANANNNAC NNCCCGNGNN NAANCCAGGN GGNNNCNCNN 961 GNNANCAGNC NNNNGNNANN GNNNNNNNNN TNNNNNNNNN N //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,EM7 pro,BleoR Sequence file: RDB15872_A8Cl.seq
>15872_15872_B3K6F_SV40pro_ori_F_G02_20_ABI24.ab1     1 NNNNNNNNNN NNGCCGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT    61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT   121 CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG   181 TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG   241 TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG   301 ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC   361 AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG   421 CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG   481 AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG   541 TGCACTTCGT GGCCGANGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG   601 CCTTCTATGA AAGGNTGGGC TTCNGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC   661 NGCGCGGGGN NCTCNTGCTG GANNNCNNCG CCCACCCCNN NNNGNTNANN GCAGNNNNNN 721 NNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi,5' LTR Sequence file: RDB15872_A8Cm.seq
>D05101A4_1_A8Cd_HIVgp-R1_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNCTTTTTT CTCTCTGCNC CACTCTTCTC TTTGCCTTGG    61 TGGGTGCTAC TCCTAATGGT TCAATTTTTA CTACTTTATA TTTATATAAT TCACTTCTCC   121 AATTGTCCCT CATATCTCCT CCTCCAGGTC TGAAGATCAG CGGCCGGCCG CTTGCTGTGC   181 GGTGGTCTTA CTTTTGTTTT GCTCTTCCTC TATCTTGTCT AAAGCTTCCT TGGTGTCTTT   241 TATCTCTATC CTTTGATGCA CACAATAGAG GGTTGCTACT GTATTATATA ATGATCTAAG   301 TTCTTCTGAT CCTGTCTGAA GGGATGGTTG TAGCTGTCCC AGTATTTGTC TACAGCCTTC   361 TGATGTTTCT AACAGGCCAG GATTAACTGC GAATCGTTCT AGCTCCCTGC TTGCCCATAC   421 TATATGTTTT AATTTATATT TTTTCTTTCC CCCTGGCCTT AACCGAATTT TTTCCCATCG   481 CGATCTAATT CTCCCCCGCT TAATACTGAC GCTCTCGCAC CCATCTCTCT CCTTCTAGCC   541 TCCGCTAGTC AAAATTTTTG GCGTACTCAC CAGTCGCCGC CCCTCGCCTC TTGCCGTGCG   601 CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT TCGCTTTCAA   661 GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGANGGAN   721 CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG   781 CTTTATTGAG GCTTAAGCAG NGGGTTCCNT ANTTANCCAG AGAGCTCCCA GGCTCAGATC   841 TGGTCTAANC NNANAGACCC NNNACAGGNA AAACGCGCNG CTTATATAGA NCNNCCNCCG   901 NANANNCCNA NCGCCCATTT GCNNNANTGG GGNNGNNNNN NNNACNANNT TTTTNGNAAA 961 GTCCNNNTNN NNTTNNGNNN NCNNANNNAN NNNCNNNNNN NNNN //
Primer: RRE_check (Pr0837) Region: RRE Sequence file: RDB15872_A8Cn.seq
>D05101A4_A8Cd_1_RRE_check_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTTCTTT TCTTGCTGGT TTTGCGANTC TTCANTTAAG    61 GAGTGTATTA AGCTTGTGTA ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA   121 TTCCAAATCT GTTCCAGAGA TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG   181 CAAATGAGTT TTCCAGAGCA ACCCCAAATC CCCAGGAGCT GTTGATCCTT TAGGTATCTT   241 TCCACAGCCA GGATTCTTGC CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA   301 TGCTGTTGCG CCTCAATAGC CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ACCAGACAAT   361 AATTGTCTGG CCTGTACCGT CAGCGTCATT GACGCTGCGC CCATAGTGCT TCCTGCTGCT   421 CCCAAGAACC CAAGGAACAA AGCTCCTATT CCCACTGCTC TTTTTTCTCT CTGCACCACT   481 CTTCTCTTTG CCTTGGTGGG TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA   541 TATAATTCAC TTCTCCAATT GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC   601 CGGCCGCTTG CTGTGCGGTG GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG   661 CTTCCTTGGT GTCTTTTATC TCTATCCTTT GATGCACACA ATAGANGGTT GCTACTGTAT   721 TATATAATGA TCTAAGTTCT TCTGATCCTG TCTGAANGGA NGGTTGTAGC TGTCCCAGTA   781 TTTGTCTACA GCCTTCTGAT GTTTCTAACA NGNCAGGATT ANCTGCGAAT CGTTCTAGCT   841 CCCTGCTTGN CCATACTATA TGTTTTAATT TANATTTTTT TCTTTNCCCC NNNNCNTTNN   901 CCNNNNNTTT TTCCNNTCGC GNTCNNANTT NNCCCCCNGC TTAANACTGN NNNTCTCNCN 961 CNNNNCNNNN NNNNNN //
Primer: cPPT+CTS_V2_F (Pr0303) Region: CMV en.2 Sequence file: RDB15872_A8Co.seq
>D05101A4_1_A8Cd_cPPT+CTS_V2_F_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNCANNNN NNNNTTACAA AAATTCAAAA TTTTCGGGTT    61 TATTACAGGG NCAGCAGAAN TTCACTTTGA ATTAATTCGA TTAATAGTAA TCAATTACGG   121 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC   181 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA   241 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG   301 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG   361 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT   421 GGCAGTACAT CTACGTATTA GTCATCNNTA TTACCATGGT GATGCGGTTT TGGCAGTACA   481 TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG   541 TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT   601 CCGCCCCATT GNCGCAAATG GGCGGTAGGC GTGTNNNGTG GGAGGTCTAT ATAAGCAGAG   661 CTGGTTTAGT GAACCGTCNN ATNNGCTAGA GCCACCATGG TANAGATGCT GNCNACTGTC   721 GCTGTGCTGG TCTTGGCAGT GTCCGTGGTT GCCAAGGATA ACACCACGCT GCANGCTANA   781 AAGAACGGCN TCNANGCGAN NTTNACGGNN NNCCACAACN NNNANGNNGG CANCGTGCAG   841 CNCNNCNANN NCTANNNNCA GNNNNNNNNN ANNNNNGANG GNNNNNNNCN GNTGNNNNNN 901 NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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