Expression vector of mouse aryl hydrocarbon receptor (AhR).
Clone info. | Expression vector of mouse aryl hydrocarbon receptor (AhR), CAG promoter, see Fig. 1A of Kimura, E. et al., PLoS One., 12 (8): e0183497, 2017. |
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Comment | A full-length AhR cDNA fragment was obtained from a mouse liver cDNA library. Expression was confirmed by the depositor with in utero electroporation and fluorescence microscopy. |
Vector backbone | pCAGGS (Plasmid) |
Size of vector backbone | 4.8 kb |
Selectable markers | Amp^r |
Gene/insert name | Mouse Ahr cDNA |
Depositor|Developer | Tohyama, Chiharu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kimura, E et al. PLoS One 12 (8): e0183497, 2017). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kimura, E et al. PLoS One 12 (8): e0183497, 2017)。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15878 | pCAGGS1-mmAhR | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCAGGS1-mmAhR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15878). |
Reference section:
Kimura, E., Kubo, K.I., Endo, T., Ling, W., Nakajima, K., Kakeyama, M., Tohyama, C., Impaired dendritic growth and positioning of cortical pyramidal neurons by activation of aryl hydrocarbon receptor signaling in the developing mouse. PLoS One 12 (8): e0183497 (2017). PMID 28820910. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15878_A8Cmp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAxCA_F1 Sequence file: RDB15878_A8Cma.seq |
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>D05162A1-1_A8Cm_pAxCA_F1_A01_01_ABI08.ab1 1 CGTCTGTCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT 61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGGGATC CCGGAGCTCC GCTCGAGGCG 121 GGCACCATGA GCAGCGGCGC CAACATCACC TATGCCAGCC GCAAGCGGCG CAAGCCGGTG 181 CAGAAAACAG TAAAGCCCAT CCCCGCTGAA GGAATTAAGT CAAATCCTTC TAAGCGACAC 241 AGAGACCGGC TGAACACAGA GTTAGACCGC CTGGCCAGCC TGCTGCCCTT CCCGCAAGAT 301 GTTATTAATA AGCTGGACAA ACTCTCTGTT CTTAGGCTCA GCGTCAGCTA CCTGAGGGCC 361 AAGAGCTTCT TTGATGTTGC ATTAAAGTCC ACCCCTGCTG ACAGAAATGG AGGCCAGGAC 421 CAGTGTAGAG CACAAATCAG AGACTGGCAG GATTTGCAAG AAGGAGAGTT CTTGTTACAG 481 GCGCTGAATG GCTTTGTGCT GGTTGTCACA GCAGATGCCT TGGTCTTCTA TGCTTCCTCC 541 ACTATCCAAG ATTACCTGGG CTTTCAGCAG TCTGATGTCA TCCATCAGAG CGTATATGAG 601 CTCATCCATA CAGAAGACCG GGCGGAATTC CAGCGCCAGC TTCACTGGGC TCTAAACCCA 661 GACTCTGCAC AAGGAGTGGA CGAAGCCCAT GGCCCTCCAC AGGCAGCAGT CTATTATACC 721 CCAGACCAGC TTCCTCCAGA GAACGCTTCT TTCATGGAGA GGTGCTTCAG GTGCCGGCTG 781 AGGTGCCTGC TGGATAATTC ATCTGGTTTT CTGGCAATGA ATTTCCAAGG GAGGTTAAAG 841 TATCTTCATG GACAGAACAA GAAAGGGAAG GACGGAGCGC TGCTTCCTCC ACAACTGGCT 901 TTGTTTGCAA TAGCTACTCC ACTTCAGCCA CCCTCCATCC TGAAAATTCG AAACCAAAAA 961 ACTTCATCTT CAGGACCAAA CACAAGCTAG ACTTCACACC TATTGGTGTG ATGCCAAGGG 1021 CAGCTTTATT CTGGGCTATA CAGAGGTAGA GCTGTGCACA GAAGAATCGG GGTACCAGGT 1081 CATTCATGCT GCAGAACTTA GTTCACTGTG CCAGAAATCC CAACAATCCC GGCCA // |
Primer: pCA-bgter Sequence file: RDB15878_A8Cmb.seq |
>D05162A1-1_A8Cm_pCA-bgter_B01_02_ABI08.ab1 1 ACCCCAGAAT GTCCATAATT TTTGGCAGAG GGAAAAAGAT CTCAGTGGTA TTTGTGAGCC 61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT 121 TAAGGAAAAA AGCGGCCGCA AAAGGAAAAC CGCTCGAGTC AACTCTGCAC CTTGCTTAGG 181 AATGCCTGAG AACCTGGAAT TTCAGAGCTG TACTGCGTCT GGTCCACAGG GGTGCGCTGT 241 GGCCCTGGCT GGCACTGATA CATGGACATG GCCCCAGCAT AGTATGCCTG AGGACTGACC 301 ATGGCGGACT GTGAGTTTAT CCCATGACTT TGGTTTTCAG GAACTTGTAA ACAACTGACA 361 AAATCTAAAT TAGAAGTAGT GGGATGCAGG GACGGTTCAA AATCCCTTCC AGGGAAGTCC 421 AACTGCACAC TCTTGGAATG TTCTGGAAGC AGAGGCTGAT TACAGGGAGC AAAGTTCTGT 481 GTGTAAGGCA CACTGTCCAC CTCTGGTTTG TAGGGAAATT CCTGAGCAGT CCCCTGTAAG 541 CTGGAAAAGA GCTGATAGTG CTTTAGTTCC TGCTGGGGAC AGTTGAAAGA CACGGGAGGG 601 GTGGGGTTCC AACTTGCAAA CGTGCCGTTG ATTTGCGTGT GCTTCGGACC CAGATCTTGC 661 TGGGGGCACA CCATCTGACA CAGCTGCTGC TGGGGCTCCA GAGCCTGCGG CGGGGGCTGC 721 TGAAGCTGTT GCTGTTGCTC TAGTTGCAGG CGCTCCTGCA GCATACAGCT TAGGTGCTGA 781 GTCACAGGCT GCTGCTGGCA AGCCGAGTTC AGCAAAGTTG AATTGTTCAG GGAATCCTGC 841 ACGTAGGTCA GGATTTCGTC CGTTATGTCG ATGTCTTTGA AGTCAACCTC ACCAGCAGCG 901 GTGGAGTCAG TTCTGAAGAA CTCCTCGTTC TGCATGCTTC TGATATCTTC AAAATCAATC 961 CCAAGGTTCC TCATGATGCT GTACAAGTCA TTATTTTTAT TATCCCGGGA AAAGAGCTCT 1021 GAGGGGCCGG CCCAGAGAGT GCAAGGATCA CGTCCCTGAG CATGGGCCAA TTGCTCATGT 1081 TTCAGCGCAG CTTCACTCCT GCGCGGCAAG CTGTTCTTTG CCGAACATTC CCGGCAT // |
Primer: RDB15879#1 Sequence file: RDB15878_A8Cmc.seq |
>D05162A1_1_A8Cm_RDB15879#1_F01_06_ABI08.ab1 1 AAGGGCGGGA AGTCAATTAA TACCCCAGGA ACCAGGCTTC CTCCAGAAGA ACGCTTCTTT 61 CATGGAGAGG TGCTTCAGGT GCCGGCTGAG GTGCCTGCTG GATAATTCAT CTGGTTTTCT 121 GGCAATGAAT TTCCAAGGGA GGTTAAAGTA TCTTCATGGA CAGAACAAGA AAGGGAAGGA 181 CGGAGCGCTG CTTCCTCCAC AACTGGCTTT GTTTGCAATA GCTACTCCAC TTCAGCCACC 241 CTCCATCCTG GAAATTCGAA CCAAAAACTT CATCTTCAGG ACCAAACACA AGCTAGACTT 301 CACACCTATT GGTTGTGATG CCAAAGGGCA GCTTATTCTG GGCTATACAG AAGTAGAGCT 361 GTGCACAAGA GGATCGGGGT ACCAGTTCAT CCATGCTGCA GACATACTTC ACTGTGCAGA 421 ATCCCACATC CGCATGATTA AGACTGGAGA AAGTGGCATG ACAGTTTTCC GGCTTCTTGC 481 AAAACACAGT CGCTGGAGGT GGGTCCAGTC CAATGCACGC TTGATTTACA GAAATGGAAG 541 ACCAGATTAC ATCATCGCCA CTCAGAGACC ACTGACGGAT GAAGAAGGAC GAGAGCATTT 601 ACAGAAGCGA AGTACGTCGC TGCCCTTCAT GTTTGCTACC GGAGAGGCTG TGTTGTACGA 661 GATCTCCAGC CCTTTCTCTC CCATAATGGA TCCCCTACCA ATACGCACCA AAAGCAACAC 721 TAGCAGGAAA GACTGGGCTC CCCAGTCAAC CCCAAGTAAG GATTCTTTCC ACCCCAGTTC 781 TCTTATGAGT GCCCTCATCC AGCAGGATGA GTCCATCTAT CTGTGTCCTC CTTCAAGCCC 841 TGCGCTGTTA GACAGCCATT TTTCTCATGG GCTCCGTGAG CAAGTGCGGG AGTTGGCAAG 901 ACAGCTTTGC GGCCGCAGGA AAGTGAGGCT GCGCTGAAAC ATGAAGCAAA TTGGCCCATG 961 CTTCAGGACG TGAACCTTGC ACTTCTCTGG GCGGCCCCCT TCAGAGCTCT TTTCCGGGAT 1021 AAATAAAAAT AAATGACTTT GTAACAGCAT CATTGAAGAA TCTTGGGGAT TGATTTTTGG 1081 AGGATATCAG GAAGCCATGC AGACGAAGGA AGTTTCTTTC AGAAATCCT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kimura, E., Impaired dendritic growth and positioning of cortical pyramidal neurons by activation of aryl hydrocarbon receptor signaling in the developing mouse. PLoS One 12 (8): e0183497 (2017). PMID 28820910. [link to RRC of NBRP] |
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