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pCAGGS1-mmAhR (#RDB15878)

Expression vector of mouse aryl hydrocarbon receptor (AhR).

Clone info. Expression vector of mouse aryl hydrocarbon receptor (AhR), CAG promoter, see Fig. 1A of Kimura, E. et al., PLoS One., 12 (8): e0183497, 2017.
Comment A full-length AhR cDNA fragment was obtained from a mouse liver cDNA library. Expression was confirmed by the depositor with in utero electroporation and fluorescence microscopy.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Ahr cDNA
Depositor|Developer Tohyama, Chiharu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kimura, E et al. PLoS One 12 (8): e0183497, 2017).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kimura, E et al. PLoS One 12 (8): e0183497, 2017)。
MTAに書く付加的使用条件:
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15878 pCAGGS1-mmAhR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS1-mmAhR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15878).

Reference section:

Kimura, E., Kubo, K.I., Endo, T., Ling, W., Nakajima, K., Kakeyama, M., Tohyama, C., Impaired dendritic growth and positioning of cortical pyramidal neurons by activation of aryl hydrocarbon receptor signaling in the developing mouse. PLoS One 12 (8): e0183497 (2017). PMID 28820910. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15878_A8Cmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB15878_A8Cma.seq check
>D05162A1-1_A8Cm_pAxCA_F1_A01_01_ABI08.ab1
    1 CGTCTGTCTA CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT
   61 TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGGGATC CCGGAGCTCC GCTCGAGGCG
  121 GGCACCATGA GCAGCGGCGC CAACATCACC TATGCCAGCC GCAAGCGGCG CAAGCCGGTG
  181 CAGAAAACAG TAAAGCCCAT CCCCGCTGAA GGAATTAAGT CAAATCCTTC TAAGCGACAC
  241 AGAGACCGGC TGAACACAGA GTTAGACCGC CTGGCCAGCC TGCTGCCCTT CCCGCAAGAT
  301 GTTATTAATA AGCTGGACAA ACTCTCTGTT CTTAGGCTCA GCGTCAGCTA CCTGAGGGCC
  361 AAGAGCTTCT TTGATGTTGC ATTAAAGTCC ACCCCTGCTG ACAGAAATGG AGGCCAGGAC
  421 CAGTGTAGAG CACAAATCAG AGACTGGCAG GATTTGCAAG AAGGAGAGTT CTTGTTACAG
  481 GCGCTGAATG GCTTTGTGCT GGTTGTCACA GCAGATGCCT TGGTCTTCTA TGCTTCCTCC
  541 ACTATCCAAG ATTACCTGGG CTTTCAGCAG TCTGATGTCA TCCATCAGAG CGTATATGAG
  601 CTCATCCATA CAGAAGACCG GGCGGAATTC CAGCGCCAGC TTCACTGGGC TCTAAACCCA
  661 GACTCTGCAC AAGGAGTGGA CGAAGCCCAT GGCCCTCCAC AGGCAGCAGT CTATTATACC
  721 CCAGACCAGC TTCCTCCAGA GAACGCTTCT TTCATGGAGA GGTGCTTCAG GTGCCGGCTG
  781 AGGTGCCTGC TGGATAATTC ATCTGGTTTT CTGGCAATGA ATTTCCAAGG GAGGTTAAAG
  841 TATCTTCATG GACAGAACAA GAAAGGGAAG GACGGAGCGC TGCTTCCTCC ACAACTGGCT
  901 TTGTTTGCAA TAGCTACTCC ACTTCAGCCA CCCTCCATCC TGAAAATTCG AAACCAAAAA
  961 ACTTCATCTT CAGGACCAAA CACAAGCTAG ACTTCACACC TATTGGTGTG ATGCCAAGGG
 1021 CAGCTTTATT CTGGGCTATA CAGAGGTAGA GCTGTGCACA GAAGAATCGG GGTACCAGGT
1081 CATTCATGCT GCAGAACTTA GTTCACTGTG CCAGAAATCC CAACAATCCC GGCCA
//
Primer: pCA-bgter
Sequence file: RDB15878_A8Cmb.seq check
>D05162A1-1_A8Cm_pCA-bgter_B01_02_ABI08.ab1
    1 ACCCCAGAAT GTCCATAATT TTTGGCAGAG GGAAAAAGAT CTCAGTGGTA TTTGTGAGCC
   61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT
  121 TAAGGAAAAA AGCGGCCGCA AAAGGAAAAC CGCTCGAGTC AACTCTGCAC CTTGCTTAGG
  181 AATGCCTGAG AACCTGGAAT TTCAGAGCTG TACTGCGTCT GGTCCACAGG GGTGCGCTGT
  241 GGCCCTGGCT GGCACTGATA CATGGACATG GCCCCAGCAT AGTATGCCTG AGGACTGACC
  301 ATGGCGGACT GTGAGTTTAT CCCATGACTT TGGTTTTCAG GAACTTGTAA ACAACTGACA
  361 AAATCTAAAT TAGAAGTAGT GGGATGCAGG GACGGTTCAA AATCCCTTCC AGGGAAGTCC
  421 AACTGCACAC TCTTGGAATG TTCTGGAAGC AGAGGCTGAT TACAGGGAGC AAAGTTCTGT
  481 GTGTAAGGCA CACTGTCCAC CTCTGGTTTG TAGGGAAATT CCTGAGCAGT CCCCTGTAAG
  541 CTGGAAAAGA GCTGATAGTG CTTTAGTTCC TGCTGGGGAC AGTTGAAAGA CACGGGAGGG
  601 GTGGGGTTCC AACTTGCAAA CGTGCCGTTG ATTTGCGTGT GCTTCGGACC CAGATCTTGC
  661 TGGGGGCACA CCATCTGACA CAGCTGCTGC TGGGGCTCCA GAGCCTGCGG CGGGGGCTGC
  721 TGAAGCTGTT GCTGTTGCTC TAGTTGCAGG CGCTCCTGCA GCATACAGCT TAGGTGCTGA
  781 GTCACAGGCT GCTGCTGGCA AGCCGAGTTC AGCAAAGTTG AATTGTTCAG GGAATCCTGC
  841 ACGTAGGTCA GGATTTCGTC CGTTATGTCG ATGTCTTTGA AGTCAACCTC ACCAGCAGCG
  901 GTGGAGTCAG TTCTGAAGAA CTCCTCGTTC TGCATGCTTC TGATATCTTC AAAATCAATC
  961 CCAAGGTTCC TCATGATGCT GTACAAGTCA TTATTTTTAT TATCCCGGGA AAAGAGCTCT
 1021 GAGGGGCCGG CCCAGAGAGT GCAAGGATCA CGTCCCTGAG CATGGGCCAA TTGCTCATGT
1081 TTCAGCGCAG CTTCACTCCT GCGCGGCAAG CTGTTCTTTG CCGAACATTC CCGGCAT
//
Primer: RDB15879#1
Sequence file: RDB15878_A8Cmc.seq check
>D05162A1_1_A8Cm_RDB15879#1_F01_06_ABI08.ab1
    1 AAGGGCGGGA AGTCAATTAA TACCCCAGGA ACCAGGCTTC CTCCAGAAGA ACGCTTCTTT
   61 CATGGAGAGG TGCTTCAGGT GCCGGCTGAG GTGCCTGCTG GATAATTCAT CTGGTTTTCT
  121 GGCAATGAAT TTCCAAGGGA GGTTAAAGTA TCTTCATGGA CAGAACAAGA AAGGGAAGGA
  181 CGGAGCGCTG CTTCCTCCAC AACTGGCTTT GTTTGCAATA GCTACTCCAC TTCAGCCACC
  241 CTCCATCCTG GAAATTCGAA CCAAAAACTT CATCTTCAGG ACCAAACACA AGCTAGACTT
  301 CACACCTATT GGTTGTGATG CCAAAGGGCA GCTTATTCTG GGCTATACAG AAGTAGAGCT
  361 GTGCACAAGA GGATCGGGGT ACCAGTTCAT CCATGCTGCA GACATACTTC ACTGTGCAGA
  421 ATCCCACATC CGCATGATTA AGACTGGAGA AAGTGGCATG ACAGTTTTCC GGCTTCTTGC
  481 AAAACACAGT CGCTGGAGGT GGGTCCAGTC CAATGCACGC TTGATTTACA GAAATGGAAG
  541 ACCAGATTAC ATCATCGCCA CTCAGAGACC ACTGACGGAT GAAGAAGGAC GAGAGCATTT
  601 ACAGAAGCGA AGTACGTCGC TGCCCTTCAT GTTTGCTACC GGAGAGGCTG TGTTGTACGA
  661 GATCTCCAGC CCTTTCTCTC CCATAATGGA TCCCCTACCA ATACGCACCA AAAGCAACAC
  721 TAGCAGGAAA GACTGGGCTC CCCAGTCAAC CCCAAGTAAG GATTCTTTCC ACCCCAGTTC
  781 TCTTATGAGT GCCCTCATCC AGCAGGATGA GTCCATCTAT CTGTGTCCTC CTTCAAGCCC
  841 TGCGCTGTTA GACAGCCATT TTTCTCATGG GCTCCGTGAG CAAGTGCGGG AGTTGGCAAG
  901 ACAGCTTTGC GGCCGCAGGA AAGTGAGGCT GCGCTGAAAC ATGAAGCAAA TTGGCCCATG
  961 CTTCAGGACG TGAACCTTGC ACTTCTCTGG GCGGCCCCCT TCAGAGCTCT TTTCCGGGAT
 1021 AAATAAAAAT AAATGACTTT GTAACAGCAT CATTGAAGAA TCTTGGGGAT TGATTTTTGG
1081 AGGATATCAG GAAGCCATGC AGACGAAGGA AGTTTCTTTC AGAAATCCT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kimura, E., Impaired dendritic growth and positioning of cortical pyramidal neurons by activation of aryl hydrocarbon receptor signaling in the developing mouse. PLoS One 12 (8): e0183497 (2017). PMID 28820910. [link to RRC of NBRP]