Resource data sheet
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pMCs-FLAG-Suv39h1-IRES-GFP (#RDB15883)

Retroviral expression vector of mouse SUV39H1, wild type.

Clone info. Retroviral expression vector of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1), wild type, tagged with N-terminal FLAG, fused with C-terminal IRES-GFP, see Fig. 3A of Shirai, A. et al., Elife, 1; 6. pii: e25317, 2017.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pMCs-IRES-GFP (plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r
Gene/insert name mouse Suv39h1 cDNA
Depositor|Developer Shinkai, Yoichi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shirai, A et al. ELIFE. (6), e25317, 2017)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shirai, A et al. ELIFE. (6), e25317, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15883 pMCs-FLAG-Suv39h1-IRES-GFP Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMCs-FLAG-Suv39h1-IRES-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15883).

Reference section:

Shirai, A., Kawaguchi, T., Shimojo, H., Muramatsu, D., Ishida-Yonetani, M., Nishimura, Y., Kimura, H., Nakayama, J.I., Shinkai, Y., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Shirai, A., Impact of nucleic acid and methylated H3K9 binding activities of Suv39h1 on its heterochromatin assembly. Elife 6. pii: e25317 (2017). PMID 28760201. [link to RRC of NBRP]