Please review the QC test results indicated by icon below as well as clone information before placing your order.

EGFR-EGFP

Expression vector of human epidermal growth factor receptor (EGFR) fused with GFP.

Sequence (full)	RDB15996zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hiroshima, M. et al., J. Mol. Biol. 430 (9), 1386-1401, 2018)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hiroshima, M. et al., J. Mol. Biol. 430 (9), 1386-1401, 2018)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15996_A8Gkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,SV40 intron Sequence file: RDB15996_A8Gka.seq
>15996_15996_B1EpF_CMV_Forward_D01_04_ABI08.ab1     1 NNNNNNNNNN NCCNNTTGNC GCNAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGG   121 AACTGAAAAA CCAGAAAGTT AACTGGTAAG TTTAGTCTTT TTGTCTTTTA TTTCAGGTCC   181 CGGATCCGGT GGTGGTGCAA ATCAAAGAAC TGCTCCTCAG TGGATGTTGC CTTTACTTCT   241 AGGCCTGTAC GGAAGTGTTA CTTCTGCTCT AAAAGCTGCT GCAGGGGGGG GGGGGGGGGG 301 N //
Primer: EGFR-N_rev1 (Pr0576) Region: EGFR 5' Sequence file: RDB15996_A8Gkb.seq
>D05656A1_A8GK_2_EGFR-N_rev1_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCTCCT GGNNGGNCTT TAAGAAGGAA AGANCATAAT    61 TCCTCTGCAC ATAGGTAATT TCCNAATTCC CAAGGACCAC CTCACAGTTA TTGAACATCC   121 TCTGGAGGCT GAGAAAATGA TCTTCAAAAG TGCCCAACTG CGTGAGCTTG TTACTCGTGC   181 CTTGGCAAAC TTTCTTTTCC TCCAGAGCCC GACTCGCCGG GCAGAGCGCA GCCAGCAGCG   241 CCAGGAGCGC TGCCCCGGCC GTCCCGGAGG GTCGCATCGC TGCTCCCCGA AGAGCTCGCT   301 CCGGCTCTCC CGATCAATAC TGGACGGAGT CAGGGGGCCG TGCGCGGTGG TTGTGGCGTT   361 GGCGGCGAGG CGGGGACTCG GGCGGACGCC GACGAGGTGG CCTGTCGTCC GGTCTGGGCG   421 GCGGCGGCCG CCGGGGCTAG CTCGGGACTC CGGCCGCCTC GGCCGCGGCG GGCGCTCACA   481 CCGTGCGGGG GGCGGAGGCT GCTGCGGCCG CGCTGCGCCG GGGGCTGCCC GGACGTCACC   541 CCCCCCCCCN NNCCCCCTGC AGCAGCTTTT AGAGCANAAG TAACACTTCC GTACAGGCCT   601 ANAAGTAAAG GCAACATCCA CTGAGGAGCA GTTCTTTGAT TTGCACCACC ACCGGATCCG   661 GGACCTGAAA TAAAANACAA AAAGACTAAA CTTACCNGTT AACTTTCNGG TTTTTCNGTT   721 CCTCNAGATC TGAGTCCGGT AGCGCTAGCG GATCTGACGG TTCNCTAAAC CAGCTCTGCT   781 TATATAGACC TCCCANNGTA CACGCCTANC GNNNNNTTGC GNCAATGGGG GCGGANNTGT   841 TACGACNNTT NGGAAAGNNC CGNNGNNTTT NGNTGNCAAA ANNAAACTCC CATTGACGNC   901 NANGGGGTGG NANNNNTNNG NAANNNCCCN NGAGNNNNAA NCCNNCTNTN CNNNNNCCNA   961 NTNGNNNGTN NNGNCNNANN CCGNNNNNCC NNNNGNNNAN TNNNGNNNGA NNNNNNNNAN 1021 NNNNNNNNN //
Primer: Venus-N_R (Pr0341) Region: EGFP 5',EGFR 3' Sequence file: RDB15996_A8Gkc.seq
>15996_15996_B1EpF_Venus-N_R_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTNN NGCCGTTTNC GTCGCCGTCC NGCTCGACCA GGATGGGCAC    61 CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTG GATCCCGGGC   121 CCGCGGTACC GTCGACTGCA GAATTCGAAG CTTTGCTCCA ATAAATTCAC TGCTTTGTGG   181 CGCGACCCTT AGGTATTCTG CATTTTCAGC TGTGGAGCCC TTAAAGATGC CATTTGGCTT   241 GGCTTCCTTG GGAAAGAAGT CCTGCTGGTA GTCAGGGTTG TCCAGGCTAA TTTGGTGGCT   301 GCCTTTCTGG GCCCAGTGGG CAGGGCTGTC GAATGTGCTG TTGACACAGG TGGGCTGGAC   361 AGTGTTGAGA TACTCGGGGT TGCCCACTGC AGTGCTGTGG GGGTCCTGGT AGTGTGGGTC   421 TCTGCTGGGC GCGGGGTTCA GAGGCTGATT GTGATAGACA GGATTCTGCA CAGAGCCAGC   481 GGGCCTTTTG GGAACGGACT GGTTTATGTA TTCAGGCACT GGGAGGAAGG TGTCGTCTAT   541 GCTGTCCTCA GTCAAGGCGC CTGTGGGGTC TGAGCTGTAT CGCTGCAAGA AGCTGTCTTC   601 CTTGATGGGA CAGCTTTGCA GCCCATTTCT ATCNATGCAA GCCACGGNGG AANTGTTGCT   661 GNNTGCNCTC NGANANCTCN NNNGGGNNTC CGTGNNGTNN NNGGNCTGNN GNANNNNCNN 721 GNNNNNNNNN N //
Primer: pAxEF R2 (Pr0059) Region: SV40 pA,EGFP 3' Sequence file: RDB15996_A8Gkd.seq
>D05656A1_A8GK_2_pAxEF R2_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN NNNNGNNNNN NTNNTTGCTN NATTTGTAAC    61 CANNTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG   121 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   181 CTGATTATGA TCTAGAGTCG CGGCCGCTTT ACTTGTACAG CTCGTCCATG CCGAGAGTGA   241 TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT   301 TGCTCAGGGC GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC   361 CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT   421 GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT   481 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT   541 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC   601 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG   661 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT   721 GCACGCCGTA NGTCAGGGTG GTCACGANGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG   781 TGCAGATGAA CTTCANGGTC AGCTTGCCGT ANGTGGCNTC GCCCTCGCCC TCGCCNGANA   841 CGCTGAACTN GTGGCCGTTT ACGTCGCCNT CCANCTNGAN CANGNATGGG CNNCACCCNN   901 GTGAACAGCT NCTCGNCCTT GNNCACCANN GNNGGCNANC NGNGGNNCCC GGGNNNNNNG 961 NNANNGTCNN NTGNNNAANN NNAANCTTNG NTNNNNNNNN NN //
Primer: SV40pro_F (Pr0110) Region: NeoR_KanR Sequence file: RDB15996_A8Gke.seq
>D05656A1_A8GK_2_SV40pro_F_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNGGANGCTT TTTTGGAGGN CTAGGCTTTT    61 GCNNNNANCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG   121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG   181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT   241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA   301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG   361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT   421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT   481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG   541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA   601 GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCNACG GCGAGGATCT CGTCGTGACC   661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGANAATG GCCGCTTTTC TGGATTCATC   721 GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA TAGCGTTNGC TACCNNNNNN   781 ATTGCTGAAN NAGCTTGGCG GCNNAATGNG CTGACCGCTT CCNCNNGCTT NACGGTATCG   841 CCGCTCCCGA NTCGCNNNGN NTCNNCTTCT ATCGCCTTCT TGACNANTTC TTCTGANCNN   901 NNNTCTGGGG NNNGAAATGN NNNNANNNAG CGACNCCCNA CCTGNCNTCN NNNGATTNCN   961 ANTNNNNNNN NNCNNNNNCN ATGAAAAGGN TGGGNNTNNN NNANNNNNTT TNNNNGGNNN 1021 NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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