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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pT7-3xFLAG-NLS-Flpe-polyA plasmid

FLAG-NLS-Flpe-myc expressing plasmid.

Catalog number RDB16011
Resource name pT7-3xFLAG-NLS-Flpe-polyA plasmid
Clone info. FLAG-NLS-Flpe-myc expressing plasmid. Applicable for direct transfection into mammalian cells (CMV promoter) and in-vitro transcriotion (T7 promoter).
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Saccharomyces cerevisiae Flpe cDNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
Other clones in our bank

External Database
Saccharomyces cerevisiae Flpe

          Reference sequence
            
           
          Sequence (full) RDB16011zzt02.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Osawa, Y. et al., Elife 10: e59759 (2021).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osawa, Y. et al., Elife 10: e59759 (2021).)。
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          RDB16011 pT7-3xFLAG-NLS-Flpe-polyA plasmid DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pT7-3xFLAG-NLS-Flpe-polyA plasmid was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16011).

          Reference section:

          Osawa, Y., Murata, K., Usui, M., Kuba, Y., Le, H.T., Mikami, N., Nakagawa, T., Daitoku, Y., Kato, K., Shawki, H.H., Ikeda, Y., Kuno, A., Morimoto, K., Tanimoto, Y., Dinh, T.T.H., Yagami, K.I., Ema, M., Yoshida, S., Takahashi, S., Mizuno, S., Sugiyama, F., EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10: e59759 (2021). PMID 33973520. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Expression regulation (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16011_B2Cpp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'(3XFLAG,SV40 NLS,Fipe 5')
          Sequence file: RDB16011_B2Cpa.seq check
          >16011_16011_B2Cp_3_CMV_Forward_E12_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGCGGTAG GCGTGNTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT
            121 TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC GGACTCTAGT AATACGACTC
            181 ACTATAGGGA GAGCCGCCAC CATGGACTAT AAGGACCACG ACGGAGACTA CAAGGATCAT
            241 GATATTGATT ACAAAGACGA TGACGATAAG GCCCCAAAGA AGAAGCGGAA GGTCGGTATC
            301 CACGGAGTCC CAGCAGCCAT GAGCCAATTT GATATATTAT GTAAAACACC ACCTAAGGTC
            361 CTGGTTCGTC AGTTTGTGGA AAGGTTTGAA AGACCTTCAG GGGAAAAAAT AGCATCATGT
            421 GCTGCTGAAC TAACCTATTT ATGTTGGATG ATTACTCATA ACGGAACAGC AATCAAGAGA
            481 GCCACATTCA TGAGCTATAA TACTATCATA AGCAATTCGC TGAGTTTCGA TATTGTCAAC
            541 AAATCACTCC NGTTTAAATA CAAGACGCAA AAAGCAACAA TTCTGGAAGC CTCATTAAAG
            601 AAATTAATTC CTGCTTGGGA ATTTACNATT ATTCCTTACA ATGGACAAAA NCATCAATCT
            661 GATATCACTG ATATTGNAAG TAGTTTGCNA NTACNGTTCG NATCATCNGN NGANNCANNN
          721 NNNNNNAANN AGCCNCANNN NAAAAANANN NNNNNNNN
          //
          Primer: HSV-pA_R (Pr0949)
          Region: insert 3'(Myc,Flpe 3')
          Sequence file: RDB16011_B2Cpb.seq check
          >16011_16011_B2Cp_3_HSV-pA_R_F12_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN NNTAGCCTCC CCCGTTTAAA CTCATTACTA
             61 ACCGGTAGGG ATCGAACCCT TTCACAGATC TTCTTCAGAA ATAAGTTTTT GTTCCACCGG
            121 TCCTATGCGT CTATTTATGT AGGATGAAAG GTAGTCTAGT ACCTCCTGTG ATATTATCCC
            181 ATTCCATGCG GGGTATCGTA TGCTTCCTTC AGCACTACCC TTTAGCTGTT CTATATGCTG
            241 CCACTCCTCA ATTGGATTAG TCTCATCCTT CAATGCTATC ATTTCCTTTG ATATTGGATC
            301 ATATGCATAG TACCGAGAAA CTAGTGCGAA GTAGTGATCA GGTATTGCTG TTATCTGATG
            361 AGTATACGTT GTCCTGGCCA CGGCAGAAGC ACGCTTATCG CTCCAATTTC CCACAACATT
            421 AGTCAACTCC GTTAGGCCCT TCATTGACAG AAATGAGGTC ATCAAATGTC TTCCAATGTG
            481 AGATTTTGGG CCATTCTTTA TAGCAAAGAT TGGATAAGGC GCATTTTTCT TCAAAGCCTT
            541 GTTGTACGAT CTGACTAAGT TATCTTTTAA TAATTGGTAT TCCTGTTTGT TGCTTGAAGA
            601 ATTGCCGGTC CTATTTACTC GTTTTANGAN TGGTTCANAG TTCCTCNAAA ATTCNTCCAA
            661 ATATACAAGT GGANCGNTCC TACCCCTTGC GCTNANNAAN TATATGTGCN NACNNACGCT
          721 NNNCTTNNNC TCTGNNNNNN NANNNNNNNN NN
          //
          Primer: f1ori_F (Pr0280)
          Region: f1 ori
          Sequence file: RDB16011_B2Cpc.seq check
          >16011_16011_B2Cp_3_f1ori_F_G12_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGTCNCGCTG CGCNNNNNNA CCACACCCGC
             61 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTG GGGATACCCC CTAGAGCCCC AGCTGCGCAG
            121 ATCTGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA
            181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT
            241 CGGTGGGGTA TCGATGTACA GCTAGCTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
          301 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTNN NNNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB16011_B2Cpd.seq check
          >16011_16011_B2Cp_3_SV40pro_ori_F_H12_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNGNNGC CTCTGCCTCT GNNCTATTCC NGAAGTAGTG AGGAGGCTTT
             61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
            121 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
            181 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
            241 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
            301 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
            361 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
            421 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
            481 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
            541 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
            601 GGTCTTGTCG ATCAGGANGA TCTGGACGAA NAGCATCANG GGCTCGCGCC AGCCGAACTG
            661 TTCGCCNGGC TCNAGGCGCG CNTGCCCNAN GGCGANGNNC TCGTCNNGNN CCNTNGNNNN
          721 GNCNNGCNNN NCNANNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Osawa, Y., EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10: e59759 (2021). PMID 33973520. [link to RRC of NBRP]

          2024.01.19

          GNP_filter3_RDBDEP_html_240118.pl