Resource data sheet
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pCX-mSema3e (#RDB16026)

Expression vector of mouse semaphorin 3E (Sema3e) with a signal sequence from the human CD8A gene, the FLAG peptide and the 6xHis tag.

Clone info. Expression vector of mouse semaphorin 3E (Sema3e) in pCX vector containing a kozak sequence, a signal sequence from the human CD8A gene, the FLAG peptide, and the 6xHis tag.
Vector backbone pCX (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name mouse Sema3e cDNA
Depositor|Developer Masu, Masayuki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 7(1), 13847, 2017). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Tsukuba University. (chizai@ilc.tsukuba.ac.jp) RECIPIENT must contact the DEPOSITOR (chizai@ilc.tsukuba.ac.jp) in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 7(1), 13847, 2017)。 非営利機関が非営利目的の教育・研究用に用いる場合以外は、筑波大学と別途MTAを締結すること(chizai@ilc.tsukuba.ac.jp)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者(chizai@ilc.tsukuba.ac.jp) と別途協議を行うこと。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB16026 pCX-mSema3e Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCX-mSema3e was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16026).

Reference section:

Okada, T., Keino-Masu, K., Nagamine, S., Kametani, F., Ohto, T., Hasegawa, M., van Kuppevelt, T.H., Kunita, S., Takahashi, S., Masu, M., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Okada, T., Desulfation of Heparan Sulfate by Sulf1 and Sulf2 Is Required for Corticospinal Tract Formation. Sci. Rep. 7 (1): 13847 (2017). PMID 29062064. [link to RRC of NBRP]