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GFP-OPTN-WT

Expression vector of human optineurin (OPTN).

Catalog number RDB16707
Resource name GFP-OPTN-WT
Clone info. Expression vector of human optineurin (OPTN), wild type, fused with enhanced monomeric EGFP at N-terminal, CMV promoter.
Comment Expression was confirmed by the deposior with fluorescence image.
Vector backbone pmEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human OPTN cDNA
Depositor|Developer Kinjo, Masataka |
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External Database
human OPTN

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al., J. Biochem. 164 (3): 223-229, 2018)。
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          RDB16707 GFP-OPTN-WT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The GFP-OPTN-WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16707).

          Reference section:

          Kitamura, A., Shimizu, H., Kinjo, M., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16707_A8Ilp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Venus-C_F2 (Pr0454)
          Region: insert 5'
          Sequence file: RDB16707_A8Ild.seq check
          >D05635A1_A8Il__3_VenusC_F2_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCTC GNNNNGNNNG AGCTGTACAA GTCCGGACTC
             61 NNGATCTCGA GCTCAAGCTT CGAATTCTAT GTCCCATCAA CCTCTCAGCT GCCTCACTGA
            121 AAAGGAGGAC AGCCCCAGTG AAAGCACAGG AAATGGACCC CCCCACCTGG CCCACCCAAA
            181 CCTGGACACG TTTACCCCGG AGGAGCTGCT GCAGCAGATG AAAGAGCTCC TGACCGAGAA
            241 CCACCAGCTG AAAGAAGCCA TGAAGCTAAA TAATCAAGCC ATGAAAGGGA GATTTGAGGA
            301 GCTTTCGGCC TGGACAGAGA AACAGAAGGA AGAACGCCAG TTTTTTGAGA TACAGAGCAA
            361 AGAAGCAAAA GAGCGTCTAA TGGCCTTGAG TCATGAGAAT GAGAAATTGA AGGAAGAGCT
            421 TGGAAAACTA AAAGGGAAAT CAGAAAGGTC ATCTGAGGAC CCCACTGATG ACTCCAGGCT
            481 TCCCAGGGCC GAAGCGGAGC AGGAAAAGGA CCAGCTCAGG ACCCAGGTGG TGAGGCTACA
            541 AGCAGAGAAG GCAGACCTGT TGGGCATCGT GTCTGAACTG CAGCTCAAGC TGAACTCCAG
            601 CGGCTCCTCA GAAGATTCCT TTGTTGAAAT TAGGATGGCT GAAGGAGAAG CAGAAGGGTC
            661 AGTAAAAGAA ATCAAGCATA GTCCTGGGCC CACGAGAACA GTCTCCACTG GCACGGCATT
            721 GTCTAAATAT AGGAGCAGAT CTGCAGATGG GGCCAAGAAT TACTTCGAAC ATGANGAGTT
            781 AACTGTGAGC CAGCTCCTGC TGTGCCTAAG GGAANGGAAT CAGAAGGTGG AGAGACTTNA
            841 AGTTGCACTC ANGGNNGCCA AAGAAAGAGT TTCANATTTT GAAAAGAAAN NNAGTAATCG
            901 TTCTGNNATT GAAANCCNGA CAGAGGGGAN CACANAGAAA GANNNNGNTG AAGANAANNN
          961 CCNNNNNANN GNNNGNANNC NNNNNNNANN CNCTNNNNCN NCCNNGNGNN NNNNNNNN
          //
          Primer: SV40polyA-R (Pr0109)
          Region: insert 3'
          Sequence file: RDB16707_A8Ile.seq check
          >D05635A1-3_A8Il_SV40polyA-R_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGTAAA ACCTCNACNA ATGTGGTATG GCTGATTATG
             61 ATCAGTTATC TAGATCCGGT GGATCCCGAA TGATGCAATC CATCACGTGA ATCTGTAACG
            121 TGTCTATGTC AGGCAGAACC TCTCCACACT TGGGGCAGGA ATGAATCGGA ATATTCCGCT
            181 GTTGCCGCCA GTCCCTGTCC TCAGCTCCTC TTTGAACAAG GTAAGCCTGC TGGTCAGAGT
            241 CACTTGTTCT CGCCCCATGA CGACTCTGCA TCTCCATCAA GGACTGCCTG CCTCCGTCTT
            301 CGAAAGCATC ATTCTCTTTC AGCAGAACTG CCAGCTGCAA TGCCAGTTGC TCCTTTTCCT
            361 CATGAATTTT CTCTCTCGCT GCTCTTTCAG CATGAAAATC AGAACAGTAA ACTTCCATCT
            421 GAGCCCTGAG GATGGTCATG GTTTCCAGGT CCTCTTCCTG CTTGGCAATG GTTTGCTTCA
            481 TTTCATCCAT TTGCAGCTGT TTGGAAGCCA GAGCCTTCTC TGCCAGTTCC AGTTTTTCAC
            541 TCAGTTCCTT CAGCACTGCC CTGTCCACTT TTTCTGACTC TTTTCTTGTT AGTTCCTCAA
            601 TTGTTTTCAA TGCATTATTA TGTTCTTGAA GAAGCTTGTT GTGTGTCATC TGTAGCACAG
            661 TTAATTTGGA CTTTTCATCC TCTGTTTTAG CCTGTTCCAT TTTGATTTCT GATAGCATGC
            721 TTTCCACTTG TAGCTCTAAC TTTTTGTTAG TATAAACAAG CTCTTGCTTT TCATTCAACT
            781 CTGATGGAAT TGCAGANTTT TTCCTTTCNA GGGCCTGACA CTTTTCTTGN AAGTCTCTTC
            841 TTCATTAGCT CANCTTCGCT GANTTTTGTA TGANCCTCTT GAAGCTCCTT AAACANAGAT
            901 GTCACCTGGA NGNNCANTGC TNNCACTTCG CTTNCNACAG TCTCCNGGCC TTTNNCTTNN
            961 NCATTCNCNT TNNNNGNGCT NCCCNNCNGN CNGGNTTTNN ANCTNNNANN NNNNTNNNTN
          1021 NTTTTNNTNN NNNANTCNGN AANNNNNTNN NTNNGNCNNN NNNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,mEGFP 5'
          Sequence file: RDB16707_A8Ilf.seq check
          >16707_16707_B3B6F_CMV-Forward_E03_15_ABI24.ab1
              1 NNNNNNNNNN NNNTTGNNGC NNNGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
            121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
            181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
            241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
            301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
            361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
            421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
            481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
            541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
            601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
            661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
            721 CCCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
            781 TCGTGACCGC CGCCNGGNTC ACTCTCGGCA TGGACGANCT GTACNAGTCC GGACTCAGAT
            841 CTCNAGCTCN AGCTTCNAAN TCTATGTCCN NTCAACCTCT CAGCTGCCTC ACTNNAAAGG
            901 ANGANNGCCC NNNNNAANGC ACNNNAAATG NNNCCCCCCN ANNNNNNCNN CCCAAANCNN
          961 NNNNNNNN
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB16707_A8Ilg.seq check
          >D05635A1-3_A8Il_SV40pro_F_V2_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNNC NNGGCTTTTG CNAAGANCGA TCNAGAGACA GGATGAGGAT
             61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
            601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            781 ANCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            841 GCCTTCTTGA CGAGTTCTTC TGANCGGGAN TCTGGGGTTC GAAATGANCG ANCAAGCGAN
            901 GCCCNACCTG CCNTCACGAN ATTTCGATTC CACCGNCGCC TTCNATGAAN NNNNGGGCTN
          961 NNNNNNCGTT TTNNNGGNNN NNNGNNNNNN GANNNNNNNN CGNNGGGNTN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitamura, A., Determination of cytoplasmic optineurin foci sizes using image correlation spectroscopy. J. Biochem. 164 (3): 223-229 (2018). PMID 29684153.
          protocol Kitamura, A., Spatial Image Correlation Spectroscopy (ICS): A Technique for Average Size Determination of Subcellular Accumulated Structures from Fluorescence Microscopic Images. Bio-protocol 10(10): e3624 (2020). PMID 33659297.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl