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pGEX6P-TDP43WT-6xHis (#RDB16710)

Expression vector of human TAR DNA-binding protein 43 (TDP43).

Clone info. Expression vector of human TAR DNA-binding protein 43 (TDP43), wild type, fused with GST at N-terminal and 6×His at C-terminal. For the constitution, please refer Fig. 1A of Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018.
Comment Expression was confirmed by the deposior with SDS-PAGE and silver staining.
Vector backbone pGEX-6P-1 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human TARDBP cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16710 pGEX6P-TDP43WT-6xHis DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX6P-TDP43WT-6xHis was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16710).

Reference section:

Kitamura, A., Shibasaki, A., Takeda, K., Suno, R., Kinjo, M., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16710_A8Ilp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 ()
Sequence file: RDB16710_A8Ila.seq check
>D05635A4-3_A8Il_pGEX-F2_B02_05_ABI24.ab1
    1 GACTAAAATT TAGTTTTCTT TTAAAAACGT ATTGGAAGCT ATCCCACAAA TTGATAAGTA
   61 CTTGAAATCC AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG
  121 TGGCGACCAT CCTCCAAAAT CGGATCTGGA AGTTCTGTTC CAGGGGCCCC TGGGATCCAT
  181 GTCTGAATAT ATTCGGGTAA CCGAAGATGA GAACGATGAG CCCATTGAAA TACCATCGGA
  241 AGACGATGGG ACGGTGCTGC TCTCCACGGT TACAGCCCAG TTTCCAGGGG CGTGTGGGCT
  301 TCGCTACAGG AATCCAGTGT CTCAGTGTAT GAGAGGTGTC CGGCTGGTAG AAGGAATTCT
  361 GCATGCCCCA GATGCTGGCT GGGGAAATCT GGTGTATGTT GTCAACTATC CAAAAGATAA
  421 CAAAAGAAAA ATGGATGAGA CAGATGCTTC ATCAGCAGTG AAAGTGAAAA GAGCAGTCCA
  481 GAAAACATCC GATTTAATAG TGTTGGGTCT CCCATGGAAA ACAACCGAAC AGGACCTGAA
  541 AGAGTATTTT AGTACCTTTG GAGAAGTTCT TATGGTGCAG GTCAAGAAAG ATCTTAAGAC
  601 TGGTCATTCA AAGGGGTTTG GCTTTGTTCG TTTTACGGAA TATGAAACAC AAGTGAAAGT
  661 AATGTCACAG CGACATATGA TAGATGGACG ATGGTGTGAC TGCAAACTTC CTAATTCTAA
  721 GCAAAGCCAA GATGAGCCTT TGAGAAGCAG AAAAGTGTTT GTGGGGCGCT GTACAGAGGA
  781 CATGACTGAG GATGAGCTGC GGGAGTTCTT CTCTCAGTAC GGGGATGTGA TGGATGTCTT
  841 CATCCCCAAG CCATTCAGGG CCTTTGCCTT TGTTACATTT GCAGATGATC AGATTGCGCA
  901 GTCTCTTTGT GGAGAGGACT TGATCATTAA AGGAATCAGC GTTCATATAT CCAATGCCGA
  961 ACCTAAGCAC AATAGCAATA GACAGTTAGA AAGAAGTGGA AAGATTTGGG TGGTAATCCA
 1021 GGTGGCTTTG GGAATCAAGG GTGGAATTTG GGTAATAAGC AGAGGGGGTG GAGCTGGTTG
 1081 GGAAACAATC AAGTAGTAAT ATGGGTGGTG GGAATGAACC TTTGGTTGCG TCAGGCATTA
 1141 ATCCCAGCAT GATGGCCTGC CGGCCCAAGC AGCACTACGG ACCGATGGGG TACTAGCTGC
1201 ATG
//
Primer: pJB8-Forward ()
Sequence file: RDB16710_A8Ilb.seq check
>D05635A4-3_A8Il_pJB8-Forward_C02_08_ABI24.ab1
    1 ACCGCCAAAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT
   61 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC
  121 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGATC
  181 GTCAGTCAGT CACGATGCGG CCGCTCGAGT CGACTTAATG GTGGTGATGG TGGTGATTCA
  241 TTCCCCAGCC AGAAGACTTA GAATCCATGC TTGAGCCAAA GCCTCCATTA AAACCACTGC
  301 CCGACCCTGC ATTGGATGCT GATCCCCAAC CAATTGCTGC ACCAGAATTA GAGCCACTAT
  361 AAGAGTTATT TCCAGAACCG AAGGCCTGGT TTGGCTCCCT CTGCATGTTG CCTTGGTTTT
  421 GGTTATTACC CGATGGGCCT GACTGGTTCT GCTGGCTGGC TAACATGCCC ATCATACCCC
  481 AACTGCTCTG TAGTGCTGCC TGGGCGGCAG CCATCATGGC TGGATTAATG CTGAACGCAC
  541 CAAAGTTCAT CCCACCACCC ATATTACTAC CTTGATTGTT TCCCAAACCA GCTCCACCCC
  601 CTCTGCTATT ACCAAATCCA CCCTGATTCC CAAAGCCACC TGGATTACCA CCAAATCTTC
  661 CACTTCTTTC TAACTGTCTA TTGCTATTGT GCTTAGGTTC GGCATTGGAT ATATGAACGC
  721 TGATTCCTTT AATGATCAAG TCCTCTCCAC AAAGAGACTG CGCAATCTGA TCATCTGCAA
  781 ATGTAACAAA GGCAAAGGCC CTGAATGGCT TGGGGATGAA GACATCCATC ACATCCCCGT
  841 ACTGAGAGAA GAACTCCCGC AGCTCATCCT CAGTCATGTC CTCTGTACAG CGCCCCACAA
  901 ACACTTTTCT GCTTCTCAAA GGCTCATCTT GGCTTTGCTT AGAATTAGGA AGTTTGCAGT
  961 CACACCATCG TCCATCTATC ATATGTCGCT GTGACATTAC TTTCACTTGT GTTTCATATT
 1021 CCGTAAAACG ACAAAGCCAA ACCCCTTTGA TGACCAGTCT TAGATCTTTT CTTGACCTGC
 1081 ACCATAGAAC TTCTCAACGT ACTAAAATAC TCTTCAAGTC TGGTCCGGTT GTTTTTCATG
1141 GGAGAACCAA CACTAATTAA AATTCGGGAG AGTTAAT
//
Primer: M13_-40 ()
Sequence file: RDB16710_A8Ilc.seq check
>D05635A4-3_A8Il_M13_-40_D02_11_ABI24.ab1
    1 TAAGGCAAGG TGAAATCCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC
   61 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA
  121 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
  181 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
  241 TGGGCGCTAG CGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA
  301 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA
  361 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT
  421 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG
  481 CGCCCAGCGC CATCTGATCG TTGGCAAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC
  541 AGCAATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC
  601 TATCGGCTGA AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG
  661 CCGAGACAGG ACTTAATGGG CCCGGCTAAC AGCGCGATTT GCTGGTGGAC CCAATGCGAC
  721 CAGATGCTCA GGCCAGTCGC GTACCGTCTT CTGGGAAGAA AATAATACTG TTGATGGGTG
  781 TCTGGTCAGA GACATCAGAA AATAACGCCG GACATTATTG CAGCAGCTTC CACACATGGC
  841 ATTCTGTCTA TCCAACCGGA TAAGTTAATG ATCACCCACT GACCGTTGCC CGAAAAATTT
  901 GGATACGCCC TTTCAGGTTT GAAGCGCCTT CGTTTTTACT CTGAACCACC AGCTGGGACC
  961 CAGATTTATA TGGGCAGAAT TTATACTCCA CAATTGCCAG CCGTTCGAGG GCCCGACTGA
 1021 GTGGAGACCG ATCCACACGC ATGATCGCCC ACGTTGTTGT CGCGCGTGAA GATATAAATT
1081 CGACGACCGG TTACTATTCG TTCTAAACAG GATGCGTCGT AC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitamura, A., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735. [link to RRC of NBRP]