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pGEX6P-TDP43WT-6xHis (#RDB16710)

Expression vector of human TAR DNA-binding protein 43 (TDP43).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kitamura, A., Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human TAR DNA-binding protein 43 (TDP43), wild type, fused with GST at N-terminal and 6×His at C-terminal. For the constitution, please refer Fig. 1A of Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018.
Comment Expression was confirmed by the deposior with SDS-PAGE and silver staining.
Vector backbone pGEX-6P-1 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Growth conditions LB+Amp, 37oC
Gene/insert name human TARDBP cDNA
Depositor|Developer Kinjo, Masataka |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)。

Catalog # Resource name Shipping form Fee
RDB16710 pGEX6P-TDP43WT-6xHis DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pGEX6P-TDP43WT-6xHis was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16710).

Reference section:

Kitamura, A., Shibasaki, A., Takeda, K., Suno, R., Kinjo, M., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kitamura, A., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735. [PubMed] [Article] [RRC of NBRP]