Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX6P-TDP43A315T-6xHis

Expression vector of human TAR DNA-binding protein 43 (TDP43), amyotrophic lateral sclerosis (ALS)-associated A315T mutant.

Catalog number RDB16711
Resource name pGEX6P-TDP43A315T-6xHis
Clone info. Expression vector of human TAR DNA-binding protein 43 (TDP43), amyotrophic lateral sclerosis (ALS)-associated A315T mutant, fused with GST at N-terminal and 6×His at C-terminal. For the constitution, please refer Fig. 1A of Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018.
Comment Expression was confirmed by the deposior with SDS-PAGE and silver staining.
Vector backbone pGEX-6P-1 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human TARDBP cDNA
Depositor|Developer Kinjo, Masataka |
Other clones in our bank

External Database
human TARDBP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16711 pGEX6P-TDP43A315T-6xHis DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kitamura, A., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16711_A8Ilp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB16711_A8Ila.seq check
          > TMWTWTAAATTWRGTTTTCTTTTTWAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTA
              1 TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC ATCCTCCAAA
             61 ATCGGATCTG GAAGTTCTGT TCCAGGGGCC CCTGGGATCC ATGTCTGAAT ATATTCGGGT
            121 AACCGAAGAT GAGAACGATG AGCCCATTGA AATACCATCG GAAGACGATG GGACGGTGCT
            181 GCTCTCCACG GTTACAGCCC AGTTTCCAGG GGCGTGTGGG CTTCGCTACA GGAATCCAGT
            241 GTCTCAGTGT ATGAGAGGTG TCCGGCTGGT AGAAGGAATT CTGCATGCCC CAGATGCTGG
            301 CTGGGGAAAT CTGGTGTATG TTGTCAACTA TCCAAAAGAT AACAAAAGAA AAATGGATGA
            361 GACAGATGCT TCATCAGCAG TGAAAGTGAA AAGAGCAGTC CAGAAAACAT CCGATTTAAT
            421 AGTGTTGGGT CTCCCATGGA AAACAACCGA ACAGGACCTG AAAGAGTATT TTAGTACCTT
            481 TGGGAGAAAG TTCTTATGGT GCAGGTCAAG AAAAGATCTT AAGACTGGGT CAATTCAAAG
            541 GGGTTTGGCT TTGTTCGTTT ACGGAAWAWG GAAACACAAG TGAAARTATG TCMCAGCGAC
            601 ATAATGATAR ATGGACGATG GTGTGACTGC AAACTTCCTA ATCTAACAAA GCAAGATGAR
            661 CCTTTGARAR CAAAAAARKG TTTKKGGGGC GCKKTACAGA RGACATGAMT GAGGAWKGAC
            721 YTGCGGGAGT TCTTYYYYCA GTAMCGGGRR WKKGGATGAW GTGYTYMWTC CAGCCATTCA
          781 GGGCTTTGCC TTTKTAMATT GCRATRKWAC AATTGCMRMT YYTTTTKGAA GAGMA
          //
          Primer: pJB8-Forward
          Sequence file: RDB16711_A8Ilb.seq check
          > ACGGGCAWATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTG
              1 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
             61 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGATC GTCAGTCAGT CACGATGCGG
            121 CCGCTCGAGT CGACTTAATG GTGGTGATGG TGGTGATTCA TTCCCCAGCC AGAAGACTTA
            181 GAATCCATGC TTGAGCCAAA GCCTCCATTA AAACCACTGC CCGACCCTGC ATTGGATGCT
            241 GATCCCCAAC CAATTGCTGC ACCAGAATTA GAGCCACTAT AAGAGTTATT TCCAGAACCG
            301 AAGGCCTGGT TTGGCTCCCT CTGCATGTTG CCTTGGTTTT GGTTATTACC CGATGGGCCT
            361 GACTGGTTCT GCTGGCTGGC TAACATGCCC ATCATACCCC AACTGCTCTG TAGTGCTGCC
            421 TGGGCGGCAG CCATCATGGC TGGATTAATG CTGAACGTAC CAAAGTTCAT CCCACCACCC
            481 ATATTACTAC CTTGATTGTT TCCCAAACCA GCTCCACCCC CTCTGCTATT ACCAAATCCA
            541 CCCTGATTCC CAAAGCCACC TGGATTACCA CCAAATCTTC CACTTCTTTC TAACTGTCTA
            601 TTGCTATTGT GCTTAGGTTC GGCATTGGAT ATATGAACGC TGATTCCTTT AATGATCAAG
            661 TCCTCTCCAC AAAGAGACTG CGCAATCTGA TCATCTGCAA ATGTAACAAA GGCAAAGGCC
            721 CTGAATGGCT TGGGGATGAA GACATCCATC ACATCCCCGT ACTGAGAGAA GAACTCCCGC
            781 AGCTCATCCT CAGTCATGTC CTCTGTACAG CGCCCCACAA ACACTTTTCT GCTTCTCAAA
            841 GGCTCATCTT GGCTTTGCTT AGAATTAGGA AGTTTGCAGT CACACCATCG TCCATCTATC
            901 ATATGTCGCT GTGACATTAC TTTCACTTGT GTTTCATATT CCGTAAAACG AACAAGCCAA
            961 ACCCCTTTGA ATGACCAGTC TTAAGATYTT TCTTGACCTG CACCATAAGA CTTCTTCAAG
           1021 TACTAAATAC TTCTTTTCAG TCTGTTCGTT KGTTTTTCCA TGGGRACCGA CACTATTAAT
          1081 CGAGTTTCTA GAACKGCTTC CTTTYAG
          //
          Primer: M13_-40
          Sequence file: RDB16711_A8Ilc.seq check
          > ARGGCCMRCKAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC
              1 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTTACATTAA
             61 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
            121 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTAGCGT GGTTTTTCTT
            181 TTCACCAGTG AGACGGGCAA CAGCTGATTG CCCTTCACCG CCTGGCCCTG AGAGAGTTGC
            241 AGCAAGCGGT CCACGCTGGT TTGCCCCAGC AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC
            301 GGCGGGATAT AACATGAGCT GTCTTCGGTA TCGTCGTATC CCACTACCGA GATATCCGCA
            361 CCAACGCGCA GCCCGGACTC GGTAATGGCG CGCATTGCGC CCAGCGCCAT CTGATCGTTG
            421 GCAACCAGCA TCGCAGTGGG AACGATGCCC TCATTCAGCA TTTGCATGGT TTGTTGAAAA
            481 CCGGACATGG CACTCCAGTC GCCTTCCCGT TCCGCTATCG GCTGAATTTG ATTGCGAGTG
            541 AGATATTTAT GCCAGCCAGC CAGACGCAGA CGCGCCGAGA CAGAACTTAA TGGGCCCGCT
            601 AACAGCGCGA TTTGCTGGTG ACCCAATGCG ACCAGATGCT CCACGCCCAG TCGCGTACCG
            661 TCTTCATGGG AGAAAATAAT ACTGTTGATG GGTGTCTGGT CAGAGACATC AAGAAATAAC
            721 GCCGGAACAT TAGTGCAGGC AGCTTCCACA GCAATGGCAT CCTGGTCATC CAGCGGATAG
            781 TTAATGATCA GCCCACTGAC GCGTTGCGCG AGAAGATTGT GCACCGCCGC TTTACAGGCT
            841 TCGACGCCGC TTCGTTCTAC CATCGACACC ACCACGCTGG CACCCAGTTG ATCGGCGCGA
            901 GATTTAATCG CCGCGACAAT TTGCGACGGC GCGTGCAGGG CCAGACTGGA GTGGCAACGC
            961 CAATCAGCAA CGACTGTTGC CCGCCAGTGT GTGCACGCGG TTGGGAATGT AATCAGCTCG
           1021 CATCGCGCTT CCACTTTTCC GCGTTTCGCA GAACGTGCTG CTGATCACCM CGCCGGGAAA
          1081 CGGCTCTGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kitamura, A., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl