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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX6P-TDP43A315T-6xHis (#RDB16711)

Expression vector of human TAR DNA-binding protein 43 (TDP43), amyotrophic lateral sclerosis (ALS)-associated A315T mutant.

Clone info. Expression vector of human TAR DNA-binding protein 43 (TDP43), amyotrophic lateral sclerosis (ALS)-associated A315T mutant, fused with GST at N-terminal and 6×His at C-terminal. For the constitution, please refer Fig. 1A of Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018.
Comment Expression was confirmed by the deposior with SDS-PAGE and silver staining.
Vector backbone pGEX-6P-1 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human TARDBP cDNA
Depositor|Developer Kinjo, Masataka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kitamura, A. et al. Biochem. Biophys. Rep., 14: 58-63, 2018)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16711 pGEX6P-TDP43A315T-6xHis DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX6P-TDP43A315T-6xHis was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16711).

Reference section:

Kitamura, A., Shibasaki, A., Takeda, K., Suno, R., Kinjo, M., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16711_A8Ilp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 ()
Sequence file: RDB16711_A8Ila.seq check
> TMWTWTAAATTWRGTTTTCTTTTTWAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTA
    1 TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC ATCCTCCAAA
   61 ATCGGATCTG GAAGTTCTGT TCCAGGGGCC CCTGGGATCC ATGTCTGAAT ATATTCGGGT
  121 AACCGAAGAT GAGAACGATG AGCCCATTGA AATACCATCG GAAGACGATG GGACGGTGCT
  181 GCTCTCCACG GTTACAGCCC AGTTTCCAGG GGCGTGTGGG CTTCGCTACA GGAATCCAGT
  241 GTCTCAGTGT ATGAGAGGTG TCCGGCTGGT AGAAGGAATT CTGCATGCCC CAGATGCTGG
  301 CTGGGGAAAT CTGGTGTATG TTGTCAACTA TCCAAAAGAT AACAAAAGAA AAATGGATGA
  361 GACAGATGCT TCATCAGCAG TGAAAGTGAA AAGAGCAGTC CAGAAAACAT CCGATTTAAT
  421 AGTGTTGGGT CTCCCATGGA AAACAACCGA ACAGGACCTG AAAGAGTATT TTAGTACCTT
  481 TGGGAGAAAG TTCTTATGGT GCAGGTCAAG AAAAGATCTT AAGACTGGGT CAATTCAAAG
  541 GGGTTTGGCT TTGTTCGTTT ACGGAAWAWG GAAACACAAG TGAAARTATG TCMCAGCGAC
  601 ATAATGATAR ATGGACGATG GTGTGACTGC AAACTTCCTA ATCTAACAAA GCAAGATGAR
  661 CCTTTGARAR CAAAAAARKG TTTKKGGGGC GCKKTACAGA RGACATGAMT GAGGAWKGAC
  721 YTGCGGGAGT TCTTYYYYCA GTAMCGGGRR WKKGGATGAW GTGYTYMWTC CAGCCATTCA
781 GGGCTTTGCC TTTKTAMATT GCRATRKWAC AATTGCMRMT YYTTTTKGAA GAGMA
//
Primer: pJB8-Forward ()
Sequence file: RDB16711_A8Ilb.seq check
> ACGGGCAWATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTG
    1 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
   61 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGATC GTCAGTCAGT CACGATGCGG
  121 CCGCTCGAGT CGACTTAATG GTGGTGATGG TGGTGATTCA TTCCCCAGCC AGAAGACTTA
  181 GAATCCATGC TTGAGCCAAA GCCTCCATTA AAACCACTGC CCGACCCTGC ATTGGATGCT
  241 GATCCCCAAC CAATTGCTGC ACCAGAATTA GAGCCACTAT AAGAGTTATT TCCAGAACCG
  301 AAGGCCTGGT TTGGCTCCCT CTGCATGTTG CCTTGGTTTT GGTTATTACC CGATGGGCCT
  361 GACTGGTTCT GCTGGCTGGC TAACATGCCC ATCATACCCC AACTGCTCTG TAGTGCTGCC
  421 TGGGCGGCAG CCATCATGGC TGGATTAATG CTGAACGTAC CAAAGTTCAT CCCACCACCC
  481 ATATTACTAC CTTGATTGTT TCCCAAACCA GCTCCACCCC CTCTGCTATT ACCAAATCCA
  541 CCCTGATTCC CAAAGCCACC TGGATTACCA CCAAATCTTC CACTTCTTTC TAACTGTCTA
  601 TTGCTATTGT GCTTAGGTTC GGCATTGGAT ATATGAACGC TGATTCCTTT AATGATCAAG
  661 TCCTCTCCAC AAAGAGACTG CGCAATCTGA TCATCTGCAA ATGTAACAAA GGCAAAGGCC
  721 CTGAATGGCT TGGGGATGAA GACATCCATC ACATCCCCGT ACTGAGAGAA GAACTCCCGC
  781 AGCTCATCCT CAGTCATGTC CTCTGTACAG CGCCCCACAA ACACTTTTCT GCTTCTCAAA
  841 GGCTCATCTT GGCTTTGCTT AGAATTAGGA AGTTTGCAGT CACACCATCG TCCATCTATC
  901 ATATGTCGCT GTGACATTAC TTTCACTTGT GTTTCATATT CCGTAAAACG AACAAGCCAA
  961 ACCCCTTTGA ATGACCAGTC TTAAGATYTT TCTTGACCTG CACCATAAGA CTTCTTCAAG
 1021 TACTAAATAC TTCTTTTCAG TCTGTTCGTT KGTTTTTCCA TGGGRACCGA CACTATTAAT
1081 CGAGTTTCTA GAACKGCTTC CTTTYAG
//
Primer: M13_-40 ()
Sequence file: RDB16711_A8Ilc.seq check
> ARGGCCMRCKAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATAC
    1 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTTACATTAA
   61 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
  121 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTAGCGT GGTTTTTCTT
  181 TTCACCAGTG AGACGGGCAA CAGCTGATTG CCCTTCACCG CCTGGCCCTG AGAGAGTTGC
  241 AGCAAGCGGT CCACGCTGGT TTGCCCCAGC AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC
  301 GGCGGGATAT AACATGAGCT GTCTTCGGTA TCGTCGTATC CCACTACCGA GATATCCGCA
  361 CCAACGCGCA GCCCGGACTC GGTAATGGCG CGCATTGCGC CCAGCGCCAT CTGATCGTTG
  421 GCAACCAGCA TCGCAGTGGG AACGATGCCC TCATTCAGCA TTTGCATGGT TTGTTGAAAA
  481 CCGGACATGG CACTCCAGTC GCCTTCCCGT TCCGCTATCG GCTGAATTTG ATTGCGAGTG
  541 AGATATTTAT GCCAGCCAGC CAGACGCAGA CGCGCCGAGA CAGAACTTAA TGGGCCCGCT
  601 AACAGCGCGA TTTGCTGGTG ACCCAATGCG ACCAGATGCT CCACGCCCAG TCGCGTACCG
  661 TCTTCATGGG AGAAAATAAT ACTGTTGATG GGTGTCTGGT CAGAGACATC AAGAAATAAC
  721 GCCGGAACAT TAGTGCAGGC AGCTTCCACA GCAATGGCAT CCTGGTCATC CAGCGGATAG
  781 TTAATGATCA GCCCACTGAC GCGTTGCGCG AGAAGATTGT GCACCGCCGC TTTACAGGCT
  841 TCGACGCCGC TTCGTTCTAC CATCGACACC ACCACGCTGG CACCCAGTTG ATCGGCGCGA
  901 GATTTAATCG CCGCGACAAT TTGCGACGGC GCGTGCAGGG CCAGACTGGA GTGGCAACGC
  961 CAATCAGCAA CGACTGTTGC CCGCCAGTGT GTGCACGCGG TTGGGAATGT AATCAGCTCG
 1021 CATCGCGCTT CCACTTTTCC GCGTTTCGCA GAACGTGCTG CTGATCACCM CGCCGGGAAA
1081 CGGCTCTGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kitamura, A., Analysis of the substrate recognition state of TDP-43 to single-stranded DNA using fluorescence correlation spectroscopy. Biochem. Biophys. Rep. 14: 58-63 (2018). PMID 29872735. [link to RRC of NBRP]