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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-PLCD1-PH

Expression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1).

Catalog number RDB16764
Resource name pDEST/mCherry-PLCD1-PH
Clone info. Expression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1) fused with mCherry at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence. Insert gene, from HeLa cell cDNA by PCR amplification. Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human PLCD1 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
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External Database
human PLCD1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012)の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16764 pDEST/mCherry-PLCD1-PH DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16764_A8Kmp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB16764_A8Kma.seq check
          >D05982A1_A8Km_1_CMV_Forward_A08_02_ABI24.ab1
              1 TCGTACAACT CCCGCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGTTAAGCT ATCAGGTACC ATGGTGAGCA
            181 AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT GCGCTTCAAG GTGCACATGG
            241 AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG
            301 AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG
            361 ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA CGTGAAGCAC CCCGCCGACA
            421 TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT
            481 TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCGAGTTCA
            541 TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA
            601 AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG
            661 GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCT GAGGTCAAGA
            721 CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT
            781 TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG
            841 GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGAA GCTTATGGAC TCGGGCCGGG
            901 ACTTCCTGAC CCTGCACGGC CTACAGGATG ATGAGGATCT ACAGGCGCTG CTGAAGGGCA
            961 GCCAGCTCCT GAAAGGTGAA GTCCAGCTCA TGGAGGAGAG AGCGCTTCTA CAAGTTTGCA
           1021 GGAGGACTGC AAGACCAATC TGGCAGAGTC CCGCAAGGTC ATGCGGACCC CGGGAGTCCC
           1081 AGCTGTTCTC ATCGAGGACA TCCAGGAAGT TGCGATTGGG CACGGCACGG AGGGATCTTG
          1141 GGAGAAAGGT TCGC
          //
          Primer: F1ori_F
          Sequence file: RDB16764_A8Kmb.seq check
          >D05982A1_A8Km_1_f1ori_F_A11_01_ABI08.ab1
              1 GGGGGCGCCT GGGGCCGCTG GGCAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG
             61 CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT GGGGATACCC CCTAGAGCCC CAGCTGGTTC
            121 TTTCCGCCTC AGAAGCCATA GAGCCCACCG CATCCCCAGC ATGCCTGCTA TTGTCTTCCC
            181 AATCCTCCCC CTTGCTGTCC TGCCCCACCC CACCCCCCAG AATAGAATGA CACCTACTCA
            241 GACAATGCGA TGCAATTTCC TCATTTTATT AGGAAAGGAC AGTGGGAGTG GCACCTTCCA
            301 GGGTCAAGGA AGGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG
            361 TCGAGGCTGA TCAGCGGGTT TAAACCTCGA GTCACTTCTG ACGCTGGTCC ATGGAGCCTG
            421 AGTGGTGGAT GATCTTGTGC AGCCCCAGCA CCCAGTGCTG GGCATCAGCT GGCGATGGGG
            481 CGATGAGGTC TAGTGTATTG CGCTGGTCCT TGAAGACAAT GGAGAAGCAG CGGTCCTCGG
            541 GCACATCACG GGCGAACTTC TCCAGACCCT CCGTGCGGTG CCCCATTCGC ACCTCCTGAA
            601 TGTCCTCGAT GGAGAACAGC TGGGACTCCG GGGTCCGCAT GACCTTGCGG GACTCCTGCC
            661 AGATGGTCTT GCAGTCCTCC TGCAACTTGT AGAAGCGCTC TCTCCTCCAT GAGCTGGACT
            721 TCACCTTCAG GAGCTGGCTG CCCTTCAGCA GCGCCTGTAG ATCCTCATCA TCCTGTAGGC
            781 CGTGCAGGGT CAGGAAGTCC CGGCCCGAGT CCATAAGCTT CTTGTACAGC TCGTCCATGC
            841 CGCCGGTGGA GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT
            901 TGTGGAGGTG ATGTCCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT
            961 CTTGGCCCTT GTAGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG
           1021 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGAGGCC
           1081 TCCCAGCCCA TGATCTTCTT CTGCATTTAC GGGGTCGTCG AAGGGAGGTT GATTGCCGCG
          1141 CAAGCCTTCC ACCTTGCTAG AATTGAACTT CGCCCGTTCT G
          //
          Primer: SV40pro_F
          Sequence file: RDB16764_A8Kmc.seq check
          >D05982A1_A8Km_1_SV40pro_F_D08_11_ABI24.ab1
              1 ACTCGTCCCG GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
             61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
            121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
            661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA
            961 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
           1021 TTCGGAATCG TTTTCCCGGG ACGCCGGGCT GGATGATCCT CCAGCGCGGG GATCTTCATG
           1081 CTGCAGGTCT TCGCCCACCC CAACTGGTTA ATGGCAGCCT TAATAATTGT TTACAAAATA
          1141 AAGCAATTAG GCCCATTCCA CAGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

          2022.07.12

          GNP_filter3_RDBDEP_html_220520.pl