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pCaMKIIa-PsChR-Venus

Mammalian expression vector encoding a channelrhodopsin from Platymonas (Tetraselmis) subcordiformis (PsChR).

Catalog number RDB16796
Resource name pCaMKIIa-PsChR-Venus
Clone info. Mammalian expression vector encoding a channelrhodopsin from Platymonas (Tetraselmis) subcordiformis (PsChR) tagged with Venus at the C-terminus under CaMKIIa promoter.
Comment Expression was confirmed by the depositor with whole-cell patch-clamp recording.
Vector backbone pCaMKIIa-Venus (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Platymonas subcordiformis PsChR (codon optimized for human) cDNA
Depositor|Developer Ishizuka, Toru |
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External Database
Platymonas subcordiformis PsChR (codon optimized for human)
            Reference sequence
              JX983143
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hososhima, S. et al., Sci. Rep. 5: 16533, 2015) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hososhima, S. et al., Sci. Rep. 5: 16533, 2015)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB16796 pCaMKIIa-PsChR-Venus Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pCaMKIIa-PsChR-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16796).

            Reference section:

            Hososhima, S., Yuasa, H., Ishizuka, T., Hoque, M.R., Yamashita, T., Yamanaka, A., Sugano, E., Tomita, H., Yawo, H., Near-infrared (NIR) up-conversion optogenetics. Sci. Rep. 5: 16533 (2015). PMID 26552717. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            Featured content

            Featured content Genetic Resources for Optgenetics Research (English text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Hososhima, S., Near-infrared (NIR) up-conversion optogenetics. Sci. Rep. 5: 16533 (2015). PMID 26552717. [link to RRC of NBRP]

            2023.11.23

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