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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK389 (mCherry2-BSD)

A plasmid for construction of C-terminal mCherry2-BSD tagging donors.

Catalog number RDB16817
Resource name pMK389 (mCherry2-BSD)
Clone info. A plasmid for construction of C-terminal mCherry2-BSD tagging donors.
Vector backbone pBluescript (plasmid)
Size of vector backbone 2.9 kb
Selectable markers Am^r (E. coli)
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Discosoma sp. RFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yesbolatova, A. et al., Methods pii: S1046-2023 (18), 30331-1, 2019)。 学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。
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            RDB16817 pMK389 (mCherry2-BSD) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pMK389 (mCherry2-BSD) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16817).

            Reference section:

            Yesbolatova, A., Natsume, T., Hayashi, K.I., Kanemaki, M.T., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Auxin Inducible Degron (AID) System (English text)
            Featured content Auxin Inducible Degron (AID) System (Japanese text)
            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB16817_A8Lpp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-M13_-100 (Pr0310)
            Region: T7 pro,insert 5'(Linker,mCherry2 5')
            Sequence file: RDB16817_A8Lpe.seq check
            >D06157B4_A8LP_2_pUC-M13_-100_E06_15_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT GGNNNNNCCN NGGTTTTCCC NGTCNCGACG TTGTAAAACG
               61 ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG AATTGGAGCT CGGATCCGGT
              121 GCAGGCGCCG CTAGCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG
              181 TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG
              241 GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG
              301 GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG
              361 GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC
              421 TTCAATTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC
              481 TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC
              541 TCCGACGGCC CCGTAATGCA GTGTCGTACC ATGGGCTGGG AGGCCTCCAC TGAGCGGATG
              601 TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC
              661 GGCCACTACG ACGCTGANGT CAAGANCACC TACAAGGCCN AGAAGCCCGT GCAGCTGCCC
              721 GGCGCCTACA ACGTCGACAT CAAGTTGGAC ATCCTTTCNC ACAACGAGGA CTACACCATC
              781 GNGGAACAGT ACGAACGCGC CGANGGCCGC CACTCCACCG GCGGCATGGA CGANCTGTAC
              841 AAGTAATCTA GATANNTGAT CATAATCAGC NNTACCACAT TTGNAGANGN TTTACTNGCT
              901 TTAAAAAAAC CNNCCNCANC NNCNCCNNGN ANCCNNAAAC NTNAAANNGN ANNGNNNNTN
            961 NNNGNTNNNT NNNNNTNNNT NNNTNGNNNN NTNANNANNN NNNNNNN
            //
            Primer: lacIpro-B_F (Pr0724)
            Region: T3 pro,insert 3'(loxP,mPGK ter)
            Sequence file: RDB16817_A8Lpf.seq check
            >16817_16817_B3C9F_lacIpro-B_F_H01_22_ABI24.ab1
                1 NNNNNNNNNN NNNNGNNCGN NNCGNGTCAG TGAGCGAGGN NNNNNNANNA NNNGCCCAAT
               61 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
              121 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA
              181 GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG
              241 ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC
              301 TCACTAAAGG GAACAAAAGC TGGGTACCGG ATCCTAGTGA ACCTCTTCGA GGGACCTAAT
              361 AACTTCGTAT AGCATACATT ATACGAAGTT ATATTAAGGG TTCCGGATCT CGACCAGCTT
              421 CTGATGGAAT TAGAACTTGG CAAAACAATA CTGAGAATGA AGTGTATGTG GAACAGAGGC
              481 TGCTGATCTC GTTCTTCAGG CTATGAAACT GACACATTTG GAAACCACAG TACTTAGAAC
              541 CACAAAGTGG GAATCAAGAG AAAAACAATG ATCCCACGAG AGATCTATAG ATCTATAGAT
              601 CATGAGTGGG AGGAATGAGC TGGNCCTTAA TTTGNTTTTG CTTGTTTAAA TTATGATATC
              661 CAACTATGAA ACATTATCAT AAAGCAATAG TAAAGAGCCT TCNGTAAAGA GCANGNATTT
              721 ATCTAATCNN NNNNNACCCC CACCCCCGTA NCTCCNATCC NTCCNTTNNA AATGNNNNAC
              781 TCTGNTCTCA CCCTTCTTAA CNAAGTATGA NNNGAAAAAC TTNCNTTTTA NNGGNNNTCT
              841 TTATNGNTTA ANANATCATC NNTTTNNGCA TCCCGGGGNN NTGANNTCNN NNANNANNCN
            901 TCCNNNNCNT NANNNNANGG NANNNNNTCN NNANNNCCNN NNNNNNNN
            //
            Primer: PGK_N_rev (Pr0243)
            Region: insert mid(loxP,SV40 pA,mCherry2 3')
            Sequence file: RDB16817_A8Lpg.seq check
            >D06157B4_A8LP_2_PGK_N_rev_G06_21_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGGNANANTT CGAGGTCGAN NTCCTAAGCT TGGCTGGACG
               61 TAAACTCCTC TTCAGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATATTAAG
              121 GGTTATTGAA TATGATCGAC GCGTTAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA
              181 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA
              241 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
              301 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
              361 CTGATTATGA TCAGTTATCT AGATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG
              421 CGGCCCTCGG CGCGTTCGTA CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAAAGGATG
              481 TCCAACTTGA TGTCGACGTT GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG
              541 GTGGTCTTGA CCTCAGCGTC GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC
              601 TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCAG TGGANGCCTC CCAGCCCATG
              661 GTACGACACT GCATTACGGG GCCGTCGGAN GGGAAGTTGG TGCCGCGCAN CTTCACCTTG
              721 TAGATGAACT CGCCGTCCTG CANGGAGGAN TCCTGNGTCA CGGTCACCAC GCCGCCGTCC
              781 TCNAAGTTCA TCACGCGCTC CCAATTGAAG CCNTCGGGGA ANGACAGCTT CNAGTANTCN
              841 GGGNNGTCNG CGGGGNGCTT CACGTNNNCT NGGANCCNNA CATGANCTGN NGGGGANAGG
              901 ANGTCCNANG CGAAGGGCNN GGGGNCNACC CNNNNNNNNC NTTCANCTTN NNNGNNNNGN
            961 NNNCNNNNNN NNGNNNNNNN NNN
            //
            Primer: PGKpro_F (Pr0249)
            Region: insert mid(BSD,mousePGK ter)
            Sequence file: RDB16817_A8Lph.seq check
            >D06157B4_A8LP_2_PGKpro_F_H06_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CGGGCCTTTC GNCCTGCATC CNTCTAGATC
               61 TCGAGCCACC ATGGCCAAGC CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC
              121 GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC
              181 TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC
              241 AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT
              301 CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT
              361 TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC
              421 AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG GAGGGCTAAT CGATGATATC
              481 AGATCCCCGG GATGCAGAAA TTGATGATCT ATTAAACAAT AAAGATGTCC ACTAAAATGG
              541 AAGTTTTTCC TGTCATACTT TGTTAAGAAG GGTGAGAACA GAGTACCTAC ATTTTGAATG
              601 GAAGGATTGG AGCTACGGGG GTGGGGGTGG GGTGGGATTA GATAAATGCC TGCTCTTTAC
              661 TGAAGGCTCT TTACTATTGC TTTATGATAA TGTTTCATAG TTGGATATCA TAATTTAAAC
              721 AAGCAAAACC AAATTAAGGG CCAGCTCATT CCTCNNANTC ATGATCTATA GATCTATAGA
              781 TCTCTCGTGN GATCATTGTT TTTCTCTTGA TTCCCACTTT GTGGTTCTAA NTACTGNGGT
              841 TTCCAAATGT GTCNGTTNCA TAGCNTGAAN NNNNNAGATC AGCANCCTCN GNTNCACATA
            901 CNNNTTCNTN NNCNGTATTG NTTTGNCAAN TNNNNANTNC NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yesbolatova, A., Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods pii: S1046-2023 (18) 30331-1 (2019). PMID 31026591. [link to RRC of NBRP]

            2024.03.26

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